BBa_K831015
1
BBa_K831015
Inducible HipA7 toxin under the control of prm promoter
2012-09-25T11:00:00Z
2015-05-08T01:13:32Z
This part was extracted of the genome of Escherichia coli K12 TH1269 using high fidelity PCR
HipA7 is a variant of HipA toxin of the HipAB toxin-antitoxin (TA) module. Two point mutations make this toxin responsible of the high persistence phenotype of the Escherichia coli K2 TH1269 strain.
false
false
_1090_
0
9809
9
It's complicated
true
None
false
Silvia J Ca??as
component2200581
1
BBa_I12007
component2200583
1
BBa_K831000
annotation2200581
1
BBa_I12007
range2200581
1
1
82
annotation2200583
1
BBa_K831000
range2200583
1
89
1411
BBa_K831000
1
BBa_K831000
HipA7 toxin
2012-09-25T11:00:00Z
2015-05-08T01:13:32Z
This part was extracted of the genome of Escherichia coli K12 TH1269 using high fidelity PCR
HipA7 is a variant of HipA toxin of the HipAB toxin-antitoxin (TA) module. Two point mutations make this toxin responsible of the high persistence phenotype of the Escherichia coli K2 TH1269 strain.
false
false
_1090_
0
9809
9
It's complicated
true
Due to its toxicity of HipA7, constructs for expression of this toxin in a E. coli chassis must be made using a inducible promoter with low leakage.
false
Silvia J Ca??as
annotation2199135
1
HipA7
range2199135
1
1
1323
BBa_I12007
1
Prm +
Modified lambda Prm promoter (OR-3 obliterated)
2004-07-14T11:00:00Z
2015-08-31T04:07:31Z
Shih and Gussin (1983)
Released HQ 2013
Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
false
true
_3_
0
103
7
In stock
false
In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
true
Hans
annotation937703
1
-35
range937703
1
48
53
annotation937702
1
OR2
range937702
1
33
49
annotation937701
1
OR1
range937701
1
9
25
annotation937704
1
mutate to obliterate OR3
range937704
1
59
65
annotation937705
1
-10
range937705
1
71
76
BBa_I12007_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
BBa_K831000_sequence
1
atgcctaaacttgtcacttggatgaacaaccagcgggtaggcgagttaacgaagttagccaacggcgcgcacacctttaagtatgcaccggagtggttagcaagccgttatgccagaccgttgtcactttcgctgccattgcagagggggaatatcacctctgatgccgtatttaacttcttcgataacctgttacccgatagcccgattgtacgtgaccggatcgttaaacgttatcatgccaaatccagacaaccgtttgatttattgtcagaaatagggcgagacagcgttggtgccgtgacgttaatacccgaagacgaaaccgtaacgcatccgataatggcatgggaaaagcttactgaagccagacttgaagaagtattaacggcttataaagcagatatcccgctaggcatgattagagaagaaaatgactttcgcatctcggttgctggcgcacaggagaagacagcactgctcagaataggcaatgactggtgcattccgaaaggaataacgccgacgacgcacatcattaaattaccgattggcgaaatcaggcagcccaatgcgacgctcgatctcagccaaagcgttgataatgagtattactgtctgctgctggcgaaagaacttgggttgaatgttccggacgcagaaatcattaaagcgggaaatgtgcgcgcgttagcggtcgaacgttttgacaggcgttggaatgctgagcgaacggttttacttcgcttgccacaggaggatatgtgtcagacattcggtttaccttcatcggtgaaatatgaatcagatggaggcccaggcatcgcgcggatcatggcttttttgatggggtccagcgaggcgctgaaagatcgctatgattttatgaaattccaggtcttccagtggttgattggcgcaacggacggtcatgcaaaaaacttctccgtatttattcaggctggcggcagttatcgactcacgccattttacgacatcatttcagcatttccggtccttggcggtacgggaatacacatcagcgatctcaaactggcaatggggcttaacgcatccaaaggcaaaaaaacggcaatcgataaaatttatccgcgacattttttggcgacagcaaaggtgctgagattcccggaagtgcagatgcatgaaatcctgagtgactttgccagaatgattccagcagcactggataacgtgaagacttcattaccgacagattttccggagaacgtggtgacggcagttgaaagcaatgtgttgaggttgcatggacggttaagccgagaatacggtagtaagtga
BBa_K831015_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagatgcctaaacttgtcacttggatgaacaaccagcgggtaggcgagttaacgaagttagccaacggcgcgcacacctttaagtatgcaccggagtggttagcaagccgttatgccagaccgttgtcactttcgctgccattgcagagggggaatatcacctctgatgccgtatttaacttcttcgataacctgttacccgatagcccgattgtacgtgaccggatcgttaaacgttatcatgccaaatccagacaaccgtttgatttattgtcagaaatagggcgagacagcgttggtgccgtgacgttaatacccgaagacgaaaccgtaacgcatccgataatggcatgggaaaagcttactgaagccagacttgaagaagtattaacggcttataaagcagatatcccgctaggcatgattagagaagaaaatgactttcgcatctcggttgctggcgcacaggagaagacagcactgctcagaataggcaatgactggtgcattccgaaaggaataacgccgacgacgcacatcattaaattaccgattggcgaaatcaggcagcccaatgcgacgctcgatctcagccaaagcgttgataatgagtattactgtctgctgctggcgaaagaacttgggttgaatgttccggacgcagaaatcattaaagcgggaaatgtgcgcgcgttagcggtcgaacgttttgacaggcgttggaatgctgagcgaacggttttacttcgcttgccacaggaggatatgtgtcagacattcggtttaccttcatcggtgaaatatgaatcagatggaggcccaggcatcgcgcggatcatggcttttttgatggggtccagcgaggcgctgaaagatcgctatgattttatgaaattccaggtcttccagtggttgattggcgcaacggacggtcatgcaaaaaacttctccgtatttattcaggctggcggcagttatcgactcacgccattttacgacatcatttcagcatttccggtccttggcggtacgggaatacacatcagcgatctcaaactggcaatggggcttaacgcatccaaaggcaaaaaaacggcaatcgataaaatttatccgcgacattttttggcgacagcaaaggtgctgagattcccggaagtgcagatgcatgaaatcctgagtgactttgccagaatgattccagcagcactggataacgtgaagacttcattaccgacagattttccggagaacgtggtgacggcagttgaaagcaatgtgttgaggttgcatggacggttaagccgagaatacggtagtaagtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z