BBa_K831015 1 BBa_K831015 Inducible HipA7 toxin under the control of prm promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:32Z This part was extracted of the genome of Escherichia coli K12 TH1269 using high fidelity PCR HipA7 is a variant of HipA toxin of the HipAB toxin-antitoxin (TA) module. Two point mutations make this toxin responsible of the high persistence phenotype of the Escherichia coli K2 TH1269 strain. false false _1090_ 0 9809 9 It's complicated true None false Silvia J Ca??as component2200581 1 BBa_I12007 component2200583 1 BBa_K831000 annotation2200581 1 BBa_I12007 range2200581 1 1 82 annotation2200583 1 BBa_K831000 range2200583 1 89 1411 BBa_K831000 1 BBa_K831000 HipA7 toxin 2012-09-25T11:00:00Z 2015-05-08T01:13:32Z This part was extracted of the genome of Escherichia coli K12 TH1269 using high fidelity PCR HipA7 is a variant of HipA toxin of the HipAB toxin-antitoxin (TA) module. Two point mutations make this toxin responsible of the high persistence phenotype of the Escherichia coli K2 TH1269 strain. false false _1090_ 0 9809 9 It's complicated true Due to its toxicity of HipA7, constructs for expression of this toxin in a E. coli chassis must be made using a inducible promoter with low leakage. false Silvia J Ca??as annotation2199135 1 HipA7 range2199135 1 1 1323 BBa_I12007 1 Prm + Modified lambda Prm promoter (OR-3 obliterated) 2004-07-14T11:00:00Z 2015-08-31T04:07:31Z Shih and Gussin (1983) Released HQ 2013 Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI) false true _3_ 0 103 7 In stock false In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions. true Hans annotation937703 1 -35 range937703 1 48 53 annotation937702 1 OR2 range937702 1 33 49 annotation937701 1 OR1 range937701 1 9 25 annotation937704 1 mutate to obliterate OR3 range937704 1 59 65 annotation937705 1 -10 range937705 1 71 76 BBa_I12007_sequence 1 gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt BBa_K831000_sequence 1 atgcctaaacttgtcacttggatgaacaaccagcgggtaggcgagttaacgaagttagccaacggcgcgcacacctttaagtatgcaccggagtggttagcaagccgttatgccagaccgttgtcactttcgctgccattgcagagggggaatatcacctctgatgccgtatttaacttcttcgataacctgttacccgatagcccgattgtacgtgaccggatcgttaaacgttatcatgccaaatccagacaaccgtttgatttattgtcagaaatagggcgagacagcgttggtgccgtgacgttaatacccgaagacgaaaccgtaacgcatccgataatggcatgggaaaagcttactgaagccagacttgaagaagtattaacggcttataaagcagatatcccgctaggcatgattagagaagaaaatgactttcgcatctcggttgctggcgcacaggagaagacagcactgctcagaataggcaatgactggtgcattccgaaaggaataacgccgacgacgcacatcattaaattaccgattggcgaaatcaggcagcccaatgcgacgctcgatctcagccaaagcgttgataatgagtattactgtctgctgctggcgaaagaacttgggttgaatgttccggacgcagaaatcattaaagcgggaaatgtgcgcgcgttagcggtcgaacgttttgacaggcgttggaatgctgagcgaacggttttacttcgcttgccacaggaggatatgtgtcagacattcggtttaccttcatcggtgaaatatgaatcagatggaggcccaggcatcgcgcggatcatggcttttttgatggggtccagcgaggcgctgaaagatcgctatgattttatgaaattccaggtcttccagtggttgattggcgcaacggacggtcatgcaaaaaacttctccgtatttattcaggctggcggcagttatcgactcacgccattttacgacatcatttcagcatttccggtccttggcggtacgggaatacacatcagcgatctcaaactggcaatggggcttaacgcatccaaaggcaaaaaaacggcaatcgataaaatttatccgcgacattttttggcgacagcaaaggtgctgagattcccggaagtgcagatgcatgaaatcctgagtgactttgccagaatgattccagcagcactggataacgtgaagacttcattaccgacagattttccggagaacgtggtgacggcagttgaaagcaatgtgttgaggttgcatggacggttaagccgagaatacggtagtaagtga BBa_K831015_sequence 1 gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagatgcctaaacttgtcacttggatgaacaaccagcgggtaggcgagttaacgaagttagccaacggcgcgcacacctttaagtatgcaccggagtggttagcaagccgttatgccagaccgttgtcactttcgctgccattgcagagggggaatatcacctctgatgccgtatttaacttcttcgataacctgttacccgatagcccgattgtacgtgaccggatcgttaaacgttatcatgccaaatccagacaaccgtttgatttattgtcagaaatagggcgagacagcgttggtgccgtgacgttaatacccgaagacgaaaccgtaacgcatccgataatggcatgggaaaagcttactgaagccagacttgaagaagtattaacggcttataaagcagatatcccgctaggcatgattagagaagaaaatgactttcgcatctcggttgctggcgcacaggagaagacagcactgctcagaataggcaatgactggtgcattccgaaaggaataacgccgacgacgcacatcattaaattaccgattggcgaaatcaggcagcccaatgcgacgctcgatctcagccaaagcgttgataatgagtattactgtctgctgctggcgaaagaacttgggttgaatgttccggacgcagaaatcattaaagcgggaaatgtgcgcgcgttagcggtcgaacgttttgacaggcgttggaatgctgagcgaacggttttacttcgcttgccacaggaggatatgtgtcagacattcggtttaccttcatcggtgaaatatgaatcagatggaggcccaggcatcgcgcggatcatggcttttttgatggggtccagcgaggcgctgaaagatcgctatgattttatgaaattccaggtcttccagtggttgattggcgcaacggacggtcatgcaaaaaacttctccgtatttattcaggctggcggcagttatcgactcacgccattttacgacatcatttcagcatttccggtccttggcggtacgggaatacacatcagcgatctcaaactggcaatggggcttaacgcatccaaaggcaaaaaaacggcaatcgataaaatttatccgcgacattttttggcgacagcaaaggtgctgagattcccggaagtgcagatgcatgaaatcctgagtgactttgccagaatgattccagcagcactggataacgtgaagacttcattaccgacagattttccggagaacgtggtgacggcagttgaaagcaatgtgttgaggttgcatggacggttaagccgagaatacggtagtaagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z