BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K831016
1
BBa_K831016
PxpsR
2012-09-25T11:00:00Z
2015-05-08T01:13:32Z
Ralstonia solanacearum AW
Promoter of xpsR (PxpsR) that responds to the Quorum Sensing signal 3 OH-PAME especific of Ralstonia solanacearum.
false
false
_1090_
0
9964
9
In stock
false
RFC10 compatible
false
Paola Reyes Caldas
annotation2200713
1
-35
range2200713
1
146
151
annotation2200712
1
-10
range2200712
1
169
175
annotation2200776
1
phcA box
range2200776
1
153
159
annotation2200744
1
phcA box
range2200744
1
163
176
annotation2200840
1
vsrD box
range2200840
1
18
39
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_E0030
1
eyfp
enhanced yellow fluorescent protein derived from A. victoria GFP
2004-03-02T12:00:00Z
2015-08-31T04:07:25Z
Modificaitons to Clontech EYFP by Reshma Shetty
Released HQ 2013
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy and Jennifer Braff
BBa_K831017
1
BBa_K831017
PxpsR-EYFP
2012-09-25T11:00:00Z
2015-05-08T01:13:32Z
PxpsR comes from Ralstonia solanascearum AW
Fluorescence reporter that responds to the presence of 3OH-PAME (Quorum Sensing signal)
false
false
_1090_
0
9964
9
In stock
false
Compatible with RFC 10
false
Paola Reyes Caldas
component2201037
1
BBa_K831016
component2201047
1
BBa_E0430
annotation2201037
1
BBa_K831016
range2201037
1
1
326
annotation2201047
1
BBa_E0430
range2201047
1
335
1212
BBa_E0430
1
BBa_E0430
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
2004-03-15T12:00:00Z
2015-08-31T04:07:26Z
Released HQ 2013
Standard YFP Output Device -LVA tag
false
true
_1_
0
24
7
In stock
false
true
Caitlin Conboy
component942902
1
BBa_B0012
component942887
1
BBa_E0030
component942885
1
BBa_B0034
component942892
1
BBa_B0010
annotation942885
1
BBa_B0034
range942885
1
1
12
annotation942902
1
BBa_B0012
range942902
1
838
878
annotation942887
1
BBa_E0030
range942887
1
19
741
annotation942892
1
BBa_B0010
range942892
1
750
829
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_E0430_sequence
1
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K831016_sequence
1
tagactgtttttgggtatcggatgggacttttgtgtgatatgggcgccgtccgggagggcaggggccggctgtgcgacatttacaaagctggcgtgatgttaaaaaatctttactctcctttagtggtatttcaggcaagcattggtgaaaggggtctggcaaacgggcatcatgtgccctatctgtgtagtgtcctttgttgttgcacggattcaataatgatatttcgggggattttgtggagattttagcgatgcttgttgggcgcatcgctggctgggctgtgaacaggcgcgcgcgcctggatgcggccggcattcaaaat
BBa_E0030_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
BBa_K831017_sequence
1
tagactgtttttgggtatcggatgggacttttgtgtgatatgggcgccgtccgggagggcaggggccggctgtgcgacatttacaaagctggcgtgatgttaaaaaatctttactctcctttagtggtatttcaggcaagcattggtgaaaggggtctggcaaacgggcatcatgtgccctatctgtgtagtgtcctttgttgttgcacggattcaataatgatatttcgggggattttgtggagattttagcgatgcttgttgggcgcatcgctggctgggctgtgaacaggcgcgcgcgcctggatgcggccggcattcaaaattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z