LOCUS BBa_K833012 999 bp DNA linear 25-Nov-2024 DEFINITION Lactate sensor derived from the MglB protein from E. coli ACCESSION BBa_K833012 VERSION BBa_K833012.1 FEATURES Location/Qualifiers misc_feature 97..99 /label=Y33K (Y10K in truncated version from Looger et al.) misc_feature 109..111 /label=Y37K (D14K in truncated version from Looger et al.) misc_feature 342..345 /label= N114K, K115L (N91K, K92L in truncated version from Looger et al.) misc_feature 541..543 /label=H175M, D177H (H152M, D154H in truncated version from Looger et al.) misc_feature 616..618 /label= W206K (W183K in trucnated version from Looger et al.) misc_feature 776..777 /label=D259A ( D236A in truncated version from Looger et al.) misc_feature 835..837 /label=N279D (N256D in truncated version from Looger et al.) misc_feature 523..525 /label= H175M (H152M in truncated version from Looger et al.) misc_feature 529..529 /label=D178H (D154H in truncated version from Looger et al.) ORIGIN 1 atgaataaga aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct 61 gcacacgctg ctgatactcg cattggtgta acaatcaaaa agtacgacaa aaactttatg 121 tctgtagtgc gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg 181 atgaatgatt ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg 241 aaaggggtga aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt 301 gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca aactggaacc gtctcgtaag 361 gcgctggata gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt 421 attcaaggcg atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa 481 gacggtcaga ttcagttcgt actgctgaaa ggtgaaccgg gcatgccgca tgcagaagca 541 aaaaccactt acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag 601 ttagataccg caatgaaaga caccgctcag gcgaaagata agatggacgc ctggctgtct 661 ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc 721 gcggttgaag cgctgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgcggcg 781 ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg cgggcaccgt actggatgat 841 gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggtaaaggt 901 gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc 961 gtagataaag acaacctggc tgaattcagc aagaaataa //