BBa_K833012 1 BBa_K833012 Lactate sensor derived from the MglB protein from E. coli 2012-10-01T11:00:00Z 2015-05-08T01:13:32Z This part was derived from site directed mutagenesis of the E. coli MglB protein. This part is a lactate sensor derived from site directed mutagenesis of the E. coli MglB protein. false false _1092_ 0 8978 9 It's complicated false Designing primers to incorporate a large number of site-directed mutations false Julianne Grose annotation2211276 1 H175M, D177H (H152M, D154H in truncated version from Looger et al.) range2211276 1 541 543 annotation2211278 1 D259A ( D236A in truncated version from Looger et al.) range2211278 1 776 777 annotation2211273 1 Y37K (D14K in truncated version from Looger et al.) range2211273 1 109 111 annotation2211280 1 H175M (H152M in truncated version from Looger et al.) range2211280 1 523 525 annotation2211281 1 D178H (D154H in truncated version from Looger et al.) range2211281 1 529 529 annotation2211279 1 N279D (N256D in truncated version from Looger et al.) range2211279 1 835 837 annotation2211272 1 Y33K (Y10K in truncated version from Looger et al.) range2211272 1 97 99 annotation2211275 1 N114K, K115L (N91K, K92L in truncated version from Looger et al.) range2211275 1 342 345 annotation2211277 1 W206K (W183K in trucnated version from Looger et al.) range2211277 1 616 618 BBa_K833012_sequence 1 atgaataagaaggtgttaaccctgtctgctgtgatggccagcatgttattcggtgccgctgcacacgctgctgatactcgcattggtgtaacaatcaaaaagtacgacaaaaactttatgtctgtagtgcgcaaggctattgagcaagatgcgaaagccgcgccagatgttcagctgctgatgaatgattctcagaatgaccagtccaagcagaacgatcagatcgacgtattgctggcgaaaggggtgaaggcactggcaatcaacctggttgacccggcagctgcgggtacggtgattgagaaagcgcgtgggcaaaacgtgccggtggttttcttcaaactggaaccgtctcgtaaggcgctggatagctacgacaaagcctactacgttggcactgactccaaagagtccggcattattcaaggcgatttgattgctaaacactgggcggcgaatcagggttgggatctgaacaaagacggtcagattcagttcgtactgctgaaaggtgaaccgggcatgccgcatgcagaagcaaaaaccacttacgtgattaaagaattgaacgataaaggcatcaaaactgaacagttacagttagataccgcaatgaaagacaccgctcaggcgaaagataagatggacgcctggctgtctggcccgaacgccaacaaaatcgaagtggttatcgccaacaacgatgcgatggcaatgggcgcggttgaagcgctgaaagcacacaacaagtccagcattccggtgtttggcgtcgcggcgctgccagaagcgctggcgctggtgaaatccggtgcactggcgggcaccgtactggatgatgctaacaaccaggcgaaagcgacctttgatctggcgaaaaacctggccgatggtaaaggtgcggctgatggcaccaactggaaaatcgacaacaaagtggtccgcgtaccttatgttggcgtagataaagacaacctggctgaattcagcaagaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z