BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898429 1 modified thr terminator range1898429 1 10 31 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 BBa_K836002 1 DGAT O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used 2012-09-17T11:00:00Z 2015-05-08T01:13:32Z The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner. This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids. Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT. false false _1095_ 0 11819 9 Not in stock false Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used. false Ricardo Alvarado component2185269 1 BBa_K836001 component2185279 1 BBa_B1006 component2185274 1 BBa_B1006 annotation2185269 1 BBa_K836001 range2185269 1 1 1380 annotation2185274 1 BBa_B1006 range2185274 1 1381 1419 annotation2185279 1 BBa_B1006 range2185279 1 1420 1458 BBa_K836001 1 DGAT O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) 2012-09-17T11:00:00Z 2015-05-08T01:13:32Z The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner. This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids. Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT. false false _1095_ 0 11819 9 Not in stock false Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (as in the rest of biobricks, the prefix and suffix are not present in the sequence). false Ricardo Alvarado annotation2185260 1 DGAT range2185260 1 1 1380 BBa_K836001_sequence 1 atgcggccactgcacccgatcgatttcatattcctgtcgctcgagaaacgacagcagcccatgcacgttggcggcctcttcctgttccagatcccggacaacgcgcctgacaccttcatccaggatctggtcaacgacattcgcatctcgaagtccatccccgtccctccgttcaacaacaagctcaacgggctcttctgggacgaggacgaagagttcgacctggaccatcacttccgccacatcgccctgccccatccgggccgtatccgggaactcctgatctacatctcgcaagagcacagcacgcttttggaccgcgcaaagccgctctggacctgcaatatcatcgagggtatcgaaggcaaccgcttcgcgatgtacttcaagatccaccacgccatggtcgacggtgtggccggcatgcgcctgatcgagaagtccctctcacatgacgttaccgaaaagtcgatcgtgcccccctggtgcgtcgagggtaagcgagcgaaacgattgcgcgagccaaagacgggcaagattaaaaaaatcatgtcgggaatcaagtcgcagctccaggccaccccgacggttatccaggagctgtcacagacggttttcaaggacatcggccggaacccggaccacgtctcctccttccaggcgccctgttctatcctgaaccagcgcgtgtcaagtagtcgccggttcgccgcccagtcgttcgatctggatcgcttccggaatattgcaaagagcctcaacgtcaccataaacgacgtggttctcgccgtgtgctccggcgcgctgcgcgcatacctgatgtcccataactccctgccatcaaaacccctgattgccatggtgcccgcgagcatcaggaatgacgacagcgatgtctccaaccgcatcaccatgatcctcgcgaatctggctacccacaaggacgacccgctccagcgcctggaaatcatccggcgatccgtgcagaacagcaagcaacgcttcaagcgcatgacgagcgatcagatcctgaattacagtgccgtcgtttacggtccggcgggtcttaacatcatctcgggtatgatgcccaagcgccaagccttcaacctcgtgatttccaacgtgcccggcccgcgcgagccgctgtactggaacggggcaaagctcgacgccctctacccggcatcgatcgtcctcgacggccaggctcttaacattaccatgacctcgtacctcgacaagctggaagtcggactcatcgcatgtcgcaacgccctaccccgtatgcaaaatctcctcacccatttagaggaggaaattcagctgtttgagggcgtgatcgcgaagcaggaggacatcaagacggccaattaataa BBa_K836002_sequence 1 atgcggccactgcacccgatcgatttcatattcctgtcgctcgagaaacgacagcagcccatgcacgttggcggcctcttcctgttccagatcccggacaacgcgcctgacaccttcatccaggatctggtcaacgacattcgcatctcgaagtccatccccgtccctccgttcaacaacaagctcaacgggctcttctgggacgaggacgaagagttcgacctggaccatcacttccgccacatcgccctgccccatccgggccgtatccgggaactcctgatctacatctcgcaagagcacagcacgcttttggaccgcgcaaagccgctctggacctgcaatatcatcgagggtatcgaaggcaaccgcttcgcgatgtacttcaagatccaccacgccatggtcgacggtgtggccggcatgcgcctgatcgagaagtccctctcacatgacgttaccgaaaagtcgatcgtgcccccctggtgcgtcgagggtaagcgagcgaaacgattgcgcgagccaaagacgggcaagattaaaaaaatcatgtcgggaatcaagtcgcagctccaggccaccccgacggttatccaggagctgtcacagacggttttcaaggacatcggccggaacccggaccacgtctcctccttccaggcgccctgttctatcctgaaccagcgcgtgtcaagtagtcgccggttcgccgcccagtcgttcgatctggatcgcttccggaatattgcaaagagcctcaacgtcaccataaacgacgtggttctcgccgtgtgctccggcgcgctgcgcgcatacctgatgtcccataactccctgccatcaaaacccctgattgccatggtgcccgcgagcatcaggaatgacgacagcgatgtctccaaccgcatcaccatgatcctcgcgaatctggctacccacaaggacgacccgctccagcgcctggaaatcatccggcgatccgtgcagaacagcaagcaacgcttcaagcgcatgacgagcgatcagatcctgaattacagtgccgtcgtttacggtccggcgggtcttaacatcatctcgggtatgatgcccaagcgccaagccttcaacctcgtgatttccaacgtgcccggcccgcgcgagccgctgtactggaacggggcaaagctcgacgccctctacccggcatcgatcgtcctcgacggccaggctcttaacattaccatgacctcgtacctcgacaagctggaagtcggactcatcgcatgtcgcaacgccctaccccgtatgcaaaatctcctcacccatttagaggaggaaattcagctgtttgagggcgtgatcgcgaagcaggaggacatcaagacggccaattaataaaaaaaaaaaccccgcccctgacagggcggggttttttttaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z