BBa_K836000 1 LipA lipA from B. cepacia (codon usage optimized for E. coli) 2012-09-17T11:00:00Z 2015-05-08T01:13:32Z The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner. This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit). false false _1095_ 0 11819 9 Not in stock false The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used. false Ricardo Alvarado annotation2185259 1 lipA range2185259 1 1 1098 BBa_K836003 1 lipA lipA from B. cepacia (codon usage optimized for E. coli) 2012-09-17T11:00:00Z 2015-05-08T01:13:32Z The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization for E. coli (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner. This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit). The native signal peptide was not modified. false false _1095_ 0 11819 9 Not in stock false The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (not in the sequence presented here). false Ricardo Alvarado component2185335 1 BBa_B1006 component2185330 1 BBa_K836000 annotation2185335 1 BBa_B1006 range2185335 1 1099 1137 annotation2185330 1 BBa_K836000 range2185330 1 1 1098 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K836003_sequence 1 atggctcgtacgatgagatcccgtgtagtggctggcgccgttgcatgcgcgatgtccatagccccttttgcgggcactaccgcagttatgacgctggcgacaacccatgcggcaatggccgccactgctccggctgccggatacgctgccactagatatcctattatattggtccacggtctgtcggggacggacaaatacgctggagttctggagtattggtatgggatacaggaagatcttcagcagaatggtgcgactgtttacgtcgctaatttatctggctttcaaagcgacgatggccccaatggtcgaggagaacagttactggcttatgttaaaacggtgctcgccgctacaggtgccaccaaagtaaatttagtaggtcactcacaggggggactgtcttcgcgctacgtggccgctgtagctccagatttagtcgccagcgtgacgactattggtacaccacatcgggggtcagagttcgcggactttgtgcaagatgtcctcgcatatgatccgacaggactatcttctagcgtaatcgctgcctttgtgaatgtcttcggcatactaacttctagttctcacaacacaaatcaggacgcactggcagcgcttcagaccctgaccacagcacgagcggcgacgtacaatcaaaattatccgagtgctgggctaggtgcaccgggaagttgtcagactggcgcaccaaccgaaactgtcggaggcaacacccatctgctgtactcttgggccggcaccgctatacaaccaaccttgtcagtgttcggggttactggcgctactgacacatctaccttaccgttagtagatcctgccaatgtccttgatctctcaactcttgcgttgtttggaacaggtactgtgatgattaatcggggttcgggccagaatgatggtcttgtgtctaagtgttcagctttatatggcaaagttttaagtacttcatataaatggaatcacttagatgaaatcaatcagctcctgggcgtacgtggggcctatgctgaggatccagtagccgtgattaggacacacgccaatcgcctgaaactggcaggtgtgtaataaaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K836000_sequence 1 atggctcgtacgatgagatcccgtgtagtggctggcgccgttgcatgcgcgatgtccatagccccttttgcgggcactaccgcagttatgacgctggcgacaacccatgcggcaatggccgccactgctccggctgccggatacgctgccactagatatcctattatattggtccacggtctgtcggggacggacaaatacgctggagttctggagtattggtatgggatacaggaagatcttcagcagaatggtgcgactgtttacgtcgctaatttatctggctttcaaagcgacgatggccccaatggtcgaggagaacagttactggcttatgttaaaacggtgctcgccgctacaggtgccaccaaagtaaatttagtaggtcactcacaggggggactgtcttcgcgctacgtggccgctgtagctccagatttagtcgccagcgtgacgactattggtacaccacatcgggggtcagagttcgcggactttgtgcaagatgtcctcgcatatgatccgacaggactatcttctagcgtaatcgctgcctttgtgaatgtcttcggcatactaacttctagttctcacaacacaaatcaggacgcactggcagcgcttcagaccctgaccacagcacgagcggcgacgtacaatcaaaattatccgagtgctgggctaggtgcaccgggaagttgtcagactggcgcaccaaccgaaactgtcggaggcaacacccatctgctgtactcttgggccggcaccgctatacaaccaaccttgtcagtgttcggggttactggcgctactgacacatctaccttaccgttagtagatcctgccaatgtccttgatctctcaactcttgcgttgtttggaacaggtactgtgatgattaatcggggttcgggccagaatgatggtcttgtgtctaagtgttcagctttatatggcaaagttttaagtacttcatataaatggaatcacttagatgaaatcaatcagctcctgggcgtacgtggggcctatgctgaggatccagtagccgtgattaggacacacgccaatcgcctgaaactggcaggtgtgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z