BBa_K836007 1 nitR Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus) 2012-09-19T11:00:00Z 2015-05-08T01:13:32Z The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P72312) and then the condon usage was optimized for expression in R. opacus using the free software GeneDesigner from DNA 2.0. Protein in charge to regulate the expression of the genes put under control of nitA promoter. A Gram positive RBS (SpoVG http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was inserted at the beginning. false false _1095_ 0 11819 9 Not in stock false A spacer which consist of the scar left by the assembly of a normal suffix and a CDS prefix was inserted between RBS and CDS. false Ricardo Alvarado & Veronica Delgado annotation2187536 1 Nitrilase regulator range2187536 1 19 981 annotation2187534 1 SpoVG range2187534 1 1 12 annotation2187535 1 Spacer range2187535 1 13 18 BBa_K836007_sequence 1 aaaggtggtgaatactagatgaacaccttcttctcctccgaccaggtctcggcgcccgaccgcgtcgcgctctggcacgacgtcatctgccgcagctacgtcccgctgaacatcaccctgaccagcgagcagcccttcatcggcaccgtctccaccggcaacctgggcaccgtccgcatcgccacgtcctcgtccctgccccagcagatcacccgcacccgccgcctgatccgccaggacgagcgcgagtacctcatggtcggcgtgcagtccgccggccacgccctcgtgcagcagcacggccgcaccgcccgcgtcggccgcggcggcctggtcttctgggacacccgccacccgtacgacatcctcttcccgaccgactggcgcatgagcgtcttccagttcccgcgctactccttcggcttcaccgaggacttcatcggccgcatgaccgccgtcaacgtcggcggcgaccgcggcatcggccgcgtggtctcctccttcatgaccagcatcaacgacgcgaccgacgccggcgacctggcggaggtcgcctccctccacaacagcgccgtcgacctgctgtccgcggcgatccggaccgagctggccgaccaggccgccgcctccgacggcctgctcgagtgcgtgctcgcctacatccgccagaacctggccgacccgaacctgtgcgcctcccagatcgccgccgagcacaacgtctccgtgcggaccctccaccgcctgttctcggccaccggccagggcgtggccgagcacatccgcaacctccgcctcgagcgcatcaagaccgagctggccgacccgaccagccggcgctacaccatcagcgccctggcgcgcaagtggggcttcctcgacccgtccaccttctcccgcgcgttcaaggacgcctacggcatcaccgcccgcgagtgggcggcctccgcctccgcctccccgaccgaggtctcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z