Lysis
type: ComponentDefinition
persistent identity: BBa K836009
display id: BBa_K836009
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis device for R. opacus
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: The majority of the parts where made by backtranslating a protein. Others are existing biobricks while some are promoters obtained from databases as NCBI. Check each individual part to know its origin.
owned by: james
owned by: myers
mutable description: Lysis construct based on the one presented by Berkeley's 2008 team BBa_K112022 (http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022. It is based on the inducible expression of a lysozyme and lambda phage's holin in order to liberate internal products of the bacterial cell in an efficient way. The induction is achieved by the addition of nitrile or a similar agent since both genes are regulated by nitA promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constitutive expression of nitR and a low constitutive expression of an antiholin are required to avoid undesired lysis.
star: false
creator: Ricardo Alvarado
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K836006
component: BBa_K836005
component: BBa_K836008
component: BBa_B1006
component: BBa_K836007
component: BBa_K143012
component: BBa_K836004
sequence annotation: BBa_K836008
sequence annotation: BBa_K836005
sequence annotation: BBa_K836006
sequence annotation: BBa_B1006
sequence annotation: BBa_K143012
sequence annotation: BBa_K836004
sequence annotation: BBa_K836007
sequence: https://synbiohub.org/public/igem/BBa_K836009_sequence/1

BBa_K836006
type: Component
persistent identity: component2187606
display id: component2187606
version: 1
top level: Lysis
definition: gpS
access: public

BBa_K836005
type: Component
persistent identity: component2187602
display id: component2187602
version: 1
top level: Lysis
definition: BBa_K836005
access: public

BBa_K836008
type: Component
persistent identity: component2187598
display id: component2187598
version: 1
top level: Lysis
definition: nitA promo
access: public

BBa_B1006
type: Component
persistent identity: component2187640
display id: component2187640
version: 1
top level: Lysis
definition: BBa_B1006
access: public

BBa_K836007
type: Component
persistent identity: component2187635
display id: component2187635
version: 1
top level: Lysis
definition: nitR
access: public

BBa_K143012
type: Component
persistent identity: component2187631
display id: component2187631
version: 1
top level: Lysis
definition: Pveg
access: public

BBa_B1006
type: Component
persistent identity: component2187628
display id: component2187628
version: 1
top level: Lysis
definition: BBa_B1006
access: public

BBa_K836004
type: Component
persistent identity: component2187623
display id: component2187623
version: 1
top level: Lysis
definition: gpS
access: public

BBa_K143012
type: Component
persistent identity: component2187619
display id: component2187619
version: 1
top level: Lysis
definition: Pveg
access: public

BBa_B1006
type: Component
persistent identity: component2187616
display id: component2187616
version: 1
top level: Lysis
definition: BBa_B1006
access: public

BBa_B1006
type: Component
persistent identity: component2187611
display id: component2187611
version: 1
top level: Lysis
definition: BBa_B1006
access: public

BBa_K836008
type: SequenceAnnotation
persistent identity: annotation2187598
display id: annotation2187598
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187598/range2187598/1
component: BBa_K836008

https://synbiohub.org/public/igem/BBa_K836009/annotation2187598/range2187598/1
type: Range
persistent identity: range2187598
display id: range2187598
version: 1
top level: Lysis
direction: direction/reverse
start: 1
end: 115
orientation: inline

BBa_K836005
type: SequenceAnnotation
persistent identity: annotation2187602
display id: annotation2187602
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187602/range2187602/1
component: BBa_K836005

https://synbiohub.org/public/igem/BBa_K836009/annotation2187602/range2187602/1
type: Range
persistent identity: range2187602
display id: range2187602
version: 1
top level: Lysis
direction: direction/reverse
start: 124
end: 1470
orientation: inline

BBa_K836006
type: SequenceAnnotation
persistent identity: annotation2187606
display id: annotation2187606
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187606/range2187606/1
component: BBa_K836006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187606/range2187606/1
type: Range
persistent identity: range2187606
display id: range2187606
version: 1
top level: Lysis
direction: direction/reverse
start: 1479
end: 1811
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187611
display id: annotation2187611
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187611/range2187611/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187611/range2187611/1
type: Range
persistent identity: range2187611
display id: range2187611
version: 1
top level: Lysis
direction: direction/reverse
start: 1820
end: 1858
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187616
display id: annotation2187616
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187616/range2187616/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187616/range2187616/1
type: Range
persistent identity: range2187616
display id: range2187616
version: 1
top level: Lysis
direction: direction/reverse
start: 1867
end: 1905
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2187619
display id: annotation2187619
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187619/range2187619/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K836009/annotation2187619/range2187619/1
type: Range
persistent identity: range2187619
display id: range2187619
version: 1
top level: Lysis
direction: direction/reverse
start: 1914
end: 2010
orientation: inline

BBa_K836004
type: SequenceAnnotation
persistent identity: annotation2187623
display id: annotation2187623
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187623/range2187623/1
component: BBa_K836004

https://synbiohub.org/public/igem/BBa_K836009/annotation2187623/range2187623/1
type: Range
persistent identity: range2187623
display id: range2187623
version: 1
top level: Lysis
direction: direction/reverse
start: 2019
end: 2356
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187628
display id: annotation2187628
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187628/range2187628/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187628/range2187628/1
type: Range
persistent identity: range2187628
display id: range2187628
version: 1
top level: Lysis
direction: direction/reverse
start: 2365
end: 2403
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2187631
display id: annotation2187631
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187631/range2187631/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K836009/annotation2187631/range2187631/1
type: Range
persistent identity: range2187631
display id: range2187631
version: 1
top level: Lysis
direction: direction/reverse
start: 2412
end: 2508
orientation: inline

BBa_K836007
type: SequenceAnnotation
persistent identity: annotation2187635
display id: annotation2187635
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187635/range2187635/1
component: BBa_K836007

https://synbiohub.org/public/igem/BBa_K836009/annotation2187635/range2187635/1
type: Range
persistent identity: range2187635
display id: range2187635
version: 1
top level: Lysis
direction: direction/reverse
start: 2517
end: 3497
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187640
display id: annotation2187640
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187640/range2187640/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187640/range2187640/1
type: Range
persistent identity: range2187640
display id: range2187640
version: 1
top level: Lysis
direction: direction/reverse
start: 3506
end: 3544
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

nitA promo
type: ComponentDefinition
persistent identity: BBa K836008
display id: BBa_K836008
version: 1
was generated by: iGEM to SBOL conversion
description: nitA promoter
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Promoter taken from NCBI (http://www.ncbi.nlm.nih.gov/nucleotide/346420896?report=genbank&log$=nuclalign&blast_rank=1&RID=W0SH1PA7016).
owned by: james
owned by: myers
mutable description: Promoter inducible by nitrile and similar agents.
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence was used as reported.
star: false
creator: Ricardo Alvarado & Veronica Delgado
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: nitA promoter
sequence: https://synbiohub.org/public/igem/BBa_K836008_sequence/1

nitA promoter
type: SequenceAnnotation
persistent identity: annotation2187537
display id: annotation2187537
version: 1
top level: nitA promo
location: https://synbiohub.org/public/igem/BBa_K836008/annotation2187537/range2187537/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K836008/annotation2187537/range2187537/1
type: Range
persistent identity: range2187537
display id: range2187537
version: 1
top level: nitA promo
direction: direction/forward
start: 1
end: 115
orientation: inline

gpS
type: ComponentDefinition
persistent identity: BBa K836004
display id: BBa_K836004
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis inhibitor isoform). The codon optimization for R. opacus (avoiding standard restriction sites EcoRI, PstI, XbaI and SpeI) was made using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
mutable description: This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (http://partsregistry.org/Part:BBa_K090506:Design)in order to obtain low concentration of the protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We wanted a low concentration of this protein since medium to large concentrations could affect or completely inhibit the lysis device. Basal but constitutive expression of this protein is needed to avoid the formation of pores in the design lysis device.
star: false
creator: Ricardo Alvarado
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_K090506
sequence annotation: Scar
sequence annotation: Lambda antiholin
sequence: https://synbiohub.org/public/igem/BBa_K836004_sequence/1

BBa_K090506
type: SequenceAnnotation
persistent identity: annotation2187515
display id: annotation2187515
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1
type: Range
persistent identity: range2187515
display id: range2187515
version: 1
top level: gpS
direction: direction/forward
start: 1
end: 11
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2187516
display id: annotation2187516
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1
type: Range
persistent identity: range2187516
display id: range2187516
version: 1
top level: gpS
direction: direction/forward
start: 12
end: 17
orientation: inline

Lambda antiholin
type: SequenceAnnotation
persistent identity: annotation2187517
display id: annotation2187517
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1
type: Range
persistent identity: range2187517
display id: range2187517
version: 1
top level: gpS
direction: direction/forward
start: 18
end: 338
orientation: inline

BBa_K836005
type: ComponentDefinition
persistent identity: BBa K836005
display id: BBa_K836005
version: 1
was generated by: iGEM to SBOL conversion
description: Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/G9FHW6) and then it was optimized for R. opacus (avoiding standard restriction sites).
owned by: james
owned by: myers
mutable description: Protein involved in the peptidoglycan degradation of the bacterial cell wall. A RBS for Gram positive bacteria (SpoVG) was added at the beginning.
discontinued: false
dominant: false
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A spacer was put between the RBS and the start codon which corresponds to the scar between a normal suffix and the prefix used for CDS to avoid RBS-CDS problems
star: false
creator: Ricardo Alvarado
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SpoVG
sequence annotation: Scar
sequence annotation: Lysozyme
sequence: https://synbiohub.org/public/igem/BBa_K836005_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187518
display id: annotation2187518
version: 1
top level: BBa_K836005
location: https://synbiohub.org/public/igem/BBa_K836005/annotation2187518/range2187518/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K836005/annotation2187518/range2187518/1
type: Range
persistent identity: range2187518
display id: range2187518
version: 1
top level: BBa_K836005
direction: direction/forward
start: 1
end: 12
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2187519
display id: annotation2187519
version: 1
top level: BBa_K836005
location: https://synbiohub.org/public/igem/BBa_K836005/annotation2187519/range2187519/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K836005/annotation2187519/range2187519/1
type: Range
persistent identity: range2187519
display id: range2187519
version: 1
top level: BBa_K836005
direction: direction/forward
start: 13
end: 18
orientation: inline

Lysozyme
type: SequenceAnnotation
persistent identity: annotation2187520
display id: annotation2187520
version: 1
top level: BBa_K836005
location: https://synbiohub.org/public/igem/BBa_K836005/annotation2187520/range2187520/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K836005/annotation2187520/range2187520/1
type: Range
persistent identity: range2187520
display id: range2187520
version: 1
top level: BBa_K836005
direction: direction/forward
start: 19
end: 1347
orientation: inline

gpS
type: ComponentDefinition
persistent identity: BBa K836006
display id: BBa_K836006
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis protein S) and optimized for R. opacus (avoiding standard restriction sites) using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
mutable description: This gene codifies for a protein that makes holes in the inner membrane of the bacterial cell. A Gram positive RBS (SpoVG, http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was added at the beginning.
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Between the RBS and the CDS a spacer consisting on a scar from a standard suffix and a preffix for CDS was inserted.
star: false
creator: Ricardo Alvarado
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: SpoVG
sequence annotation: Spacer
sequence annotation: Holin
sequence: https://synbiohub.org/public/igem/BBa_K836006_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187525
display id: annotation2187525
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1
type: Range
persistent identity: range2187525
display id: range2187525
version: 1
top level: gpS
direction: direction/forward
start: 1
end: 12
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2187527
display id: annotation2187527
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1
type: Range
persistent identity: range2187527
display id: range2187527
version: 1
top level: gpS
direction: direction/forward
start: 13
end: 18
orientation: inline

Holin
type: SequenceAnnotation
persistent identity: annotation2187528
display id: annotation2187528
version: 1
top level: gpS
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1
type: Range
persistent identity: range2187528
display id: range2187528
version: 1
top level: gpS
direction: direction/forward
start: 19
end: 333
orientation: inline

nitR
type: ComponentDefinition
persistent identity: BBa K836007
display id: BBa_K836007
version: 1
was generated by: iGEM to SBOL conversion
description: Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P72312) and then the condon usage was optimized for expression in R. opacus using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
mutable description: Protein in charge to regulate the expression of the genes put under control of nitA promoter. A Gram positive RBS (SpoVG http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was inserted at the beginning.
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A spacer which consist of the scar left by the assembly of a normal suffix and a CDS prefix was inserted between RBS and CDS.
star: false
creator: Ricardo Alvarado & Veronica Delgado
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: SpoVG
sequence annotation: Spacer
sequence annotation: Nitrilase regulator
sequence: https://synbiohub.org/public/igem/BBa_K836007_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187534
display id: annotation2187534
version: 1
top level: nitR
location: https://synbiohub.org/public/igem/BBa_K836007/annotation2187534/range2187534/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K836007/annotation2187534/range2187534/1
type: Range
persistent identity: range2187534
display id: range2187534
version: 1
top level: nitR
direction: direction/forward
start: 1
end: 12
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2187535
display id: annotation2187535
version: 1
top level: nitR
location: https://synbiohub.org/public/igem/BBa_K836007/annotation2187535/range2187535/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K836007/annotation2187535/range2187535/1
type: Range
persistent identity: range2187535
display id: range2187535
version: 1
top level: nitR
direction: direction/forward
start: 13
end: 18
orientation: inline

Nitrilase regulator
type: SequenceAnnotation
persistent identity: annotation2187536
display id: annotation2187536
version: 1
top level: nitR
location: https://synbiohub.org/public/igem/BBa_K836007/annotation2187536/range2187536/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K836007/annotation2187536/range2187536/1
type: Range
persistent identity: range2187536
display id: range2187536
version: 1
top level: nitR
direction: direction/forward
start: 19
end: 981
orientation: inline

https://synbiohub.org/public/igem/BBa_K836009_sequence/1
type: Sequence
persistent identity: BBa K836009 sequence
display id: BBa_K836009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaactcccttatgcgggtggcgcagaatgccaggacccttgtcattccacgtcaattcatgcgccttttcacctcgtactgtcctgccaaacacaagcaacggaggtacggactactagagaaaggtggtgaatactagatggcacagcggctctggccactcccgcgcgacggctacgagctcacctccggtttcggaccccgctggggcgcacagcattccgggctagacctcgcggccccggacgggactccgttctacgcctgccaggcaggtaccgttcagttcatcggttccgcgacgggatacggccaatggctggtgatcgactcgtctgacgacgaggggtcgggttgtgtcgagtacggtcacatgtggaatgcccgagcgaccggcctctccgtgggagaccacgtagaggccggacagctcatcggctacattgggtcgaacggacagtcgaccggcccccacttgcacatcacggtttgggaacgtggctataactcggtccggatcgacccggaagtctggctggcaagcgcgggctttcccggggaggcggtcgcggccccgaagcccagtaccggcgagccgatctggggaatcgacgtgtctaaccaccaggggaacttcgacttcgcggcggcaaaggcggaggggttcgtgttcgcgacccataaggtgaccgaggcgaccgggtaccgagacccctactgggcgagggcccgccaggaaatggaaaagcatttccccggccggtggggaggatacgtgttctgccgaacgaatacggacccggtgaccgaagcgcgatatttctacgacgccgtgggaggtgacacctccgtgccggtccaaatcgattacgaagacaccaccaacggaggcagcggtgatgacctctgcaaccgcgtccgcgcgtatcaagacctgggcttccgcctgctacccatctatatcccgcgctggtactggcgagaccacatgggatcccccgatctctctggcctgcccgtaccgatctggaactcgcactacgcccggggcgtggactacgcctcaaatctgtacacgcagcatggcgagaaagccgaagcatggtccgacatgggcgggaagccggtcagtattctccagttttccgagaccgccatggtagcaggcaaactcatcgacgtgaacgccttccgcgggaccgaagccgagctcgatgagctcttctcggggcaagaactcagcatgagcgccgtgcaggagattaaggactacattgacgtgcgtgtcaccaacccgatcggctcggacgtcaaggacttgagggagcaggcctgcggccagggggcccgcgatgcaggccagtataacggctggccgcagttggggaaccgcacaattgccgacgccctcgcggtcatcggagttgctctgaagatagagggcttcttcgatccgaggagcataggctactagagaaaggtggtgaatactagatgccagaaaagcacgacctactcgcagccatcctcgcggcaaaggagcagggcattggggcgatcctagctttcgcgatggcctacctccggggccgctacaacggcggtgcgttcaccaagacagtcatcgacgccactatgtgcgccatcattgcctggttcatccgagacctcctggacttcgctggactctcgtcgaacctggcgtacatcacctcagtcttcataggatacatcggcaccgactcaatcgggtctctcatcaagcggttcgcggccaagaaggccggtgtcgaggatgggcgcaaccaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K836008_sequence/1
type: Sequence
persistent identity: BBa K836008 sequence
display id: BBa_K836008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaactcccttatgcgggtggcgcagaatgccaggacccttgtcattccacgtcaattcatgcgccttttcacctcgtactgtcctgccaaacacaagcaacggaggtacggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K836004_sequence/1
type: Sequence
persistent identity: BBa K836004 sequence
display id: BBa_K836004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaggtggtgttactagatgaaaatgcctgagaaacatgacctgctcgccgcaatcctggcagccaaggaacagggcattggcgccatcctggccttcgcgatggcgtatctccgcggtcggtacaacggcggagccttcaccaagaccgtcatcgacgcaaccatgtgcgcgatcatcgcatggttcatcagagatctcctggacttcgccggactgtcgtccaatctcgcctacatcacaagtgtgttcatcgggtacatcggcacggactccatcggctcgctgatcaagcggttcgccgccaagaaggcgggggttgaagacggtcgcaaccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K836005_sequence/1
type: Sequence
persistent identity: BBa K836005 sequence
display id: BBa_K836005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaatactagatggcacagcggctctggccactcccgcgcgacggctacgagctcacctccggtttcggaccccgctggggcgcacagcattccgggctagacctcgcggccccggacgggactccgttctacgcctgccaggcaggtaccgttcagttcatcggttccgcgacgggatacggccaatggctggtgatcgactcgtctgacgacgaggggtcgggttgtgtcgagtacggtcacatgtggaatgcccgagcgaccggcctctccgtgggagaccacgtagaggccggacagctcatcggctacattgggtcgaacggacagtcgaccggcccccacttgcacatcacggtttgggaacgtggctataactcggtccggatcgacccggaagtctggctggcaagcgcgggctttcccggggaggcggtcgcggccccgaagcccagtaccggcgagccgatctggggaatcgacgtgtctaaccaccaggggaacttcgacttcgcggcggcaaaggcggaggggttcgtgttcgcgacccataaggtgaccgaggcgaccgggtaccgagacccctactgggcgagggcccgccaggaaatggaaaagcatttccccggccggtggggaggatacgtgttctgccgaacgaatacggacccggtgaccgaagcgcgatatttctacgacgccgtgggaggtgacacctccgtgccggtccaaatcgattacgaagacaccaccaacggaggcagcggtgatgacctctgcaaccgcgtccgcgcgtatcaagacctgggcttccgcctgctacccatctatatcccgcgctggtactggcgagaccacatgggatcccccgatctctctggcctgcccgtaccgatctggaactcgcactacgcccggggcgtggactacgcctcaaatctgtacacgcagcatggcgagaaagccgaagcatggtccgacatgggcgggaagccggtcagtattctccagttttccgagaccgccatggtagcaggcaaactcatcgacgtgaacgccttccgcgggaccgaagccgagctcgatgagctcttctcggggcaagaactcagcatgagcgccgtgcaggagattaaggactacattgacgtgcgtgtcaccaacccgatcggctcggacgtcaaggacttgagggagcaggcctgcggccagggggcccgcgatgcaggccagtataacggctggccgcagttggggaaccgcacaattgccgacgccctcgcggtcatcggagttgctctgaagatagagggcttcttcgatccgaggagcataggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K836006_sequence/1
type: Sequence
persistent identity: BBa K836006 sequence
display id: BBa_K836006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaatactagatgccagaaaagcacgacctactcgcagccatcctcgcggcaaaggagcagggcattggggcgatcctagctttcgcgatggcctacctccggggccgctacaacggcggtgcgttcaccaagacagtcatcgacgccactatgtgcgccatcattgcctggttcatccgagacctcctggacttcgctggactctcgtcgaacctggcgtacatcacctcagtcttcataggatacatcggcaccgactcaatcgggtctctcatcaagcggttcgcggccaagaaggccggtgtcgaggatgggcgcaaccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K836007_sequence/1
type: Sequence
persistent identity: BBa K836007 sequence
display id: BBa_K836007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaatactagatgaacaccttcttctcctccgaccaggtctcggcgcccgaccgcgtcgcgctctggcacgacgtcatctgccgcagctacgtcccgctgaacatcaccctgaccagcgagcagcccttcatcggcaccgtctccaccggcaacctgggcaccgtccgcatcgccacgtcctcgtccctgccccagcagatcacccgcacccgccgcctgatccgccaggacgagcgcgagtacctcatggtcggcgtgcagtccgccggccacgccctcgtgcagcagcacggccgcaccgcccgcgtcggccgcggcggcctggtcttctgggacacccgccacccgtacgacatcctcttcccgaccgactggcgcatgagcgtcttccagttcccgcgctactccttcggcttcaccgaggacttcatcggccgcatgaccgccgtcaacgtcggcggcgaccgcggcatcggccgcgtggtctcctccttcatgaccagcatcaacgacgcgaccgacgccggcgacctggcggaggtcgcctccctccacaacagcgccgtcgacctgctgtccgcggcgatccggaccgagctggccgaccaggccgccgcctccgacggcctgctcgagtgcgtgctcgcctacatccgccagaacctggccgacccgaacctgtgcgcctcccagatcgccgccgagcacaacgtctccgtgcggaccctccaccgcctgttctcggccaccggccagggcgtggccgagcacatccgcaacctccgcctcgagcgcatcaagaccgagctggccgacccgaccagccggcgctacaccatcagcgccctggcgcgcaagtggggcttcctcgacccgtccaccttctcccgcgcgttcaaggacgcctacggcatcaccgcccgcgagtgggcggcctccgcctccgcctccccgaccgaggtctcgtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z