BBa_K837000 1 BBa_K837000 RNAT.1-eGFP-pSB1C3 2012-09-25T11:00:00Z 2015-05-08T01:13:33Z The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891. The part include a RNAT, which is a temperature-sensing sequence, in front of the reporter eGFP. As the environmental temperature changes below 30℃, the RNAT folds into a series of different secondary structures and block ribosomes??? access to the mRNA thus hinder translation of the eGFP. false false _1096_ 0 12280 9 It's complicated false The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence. false Sun Xiaochen annotation2200762 1 Preffix range2200762 1 790 810 annotation2200737 1 RNAT.1 range2200737 1 23 69 annotation2200736 1 EGFP range2200736 1 70 789 annotation2200735 1 SD range2200735 1 55 61 annotation2200749 1 Suffix range2200749 1 1 22 BBa_K837000_sequence 1 gaattcgcggccgcttctagagacacggatctactagcgtgaatttatcacgggaagaagtcgccgtaaatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z