BBa_K837001 1 BBa_K837001 RNAT.2-eGFP-pSB1C3 2012-09-25T11:00:00Z 2015-05-08T01:13:33Z The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891. The part includes an RNAT, which is a temperature-sensing sequence, in front of the reporter eGFP. As the environmental temperature changes below 30℃, the RNAT folds into a series of different secondary tructures and block ribosomes??? access to the mRNA thus hinder translation of eGFP. false false _1096_ 0 12280 9 It's complicated false The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence. false Sun Xiaochen annotation2201057 1 SD range2201057 1 57 63 annotation2201059 1 Preffix range2201059 1 788 808 annotation2201058 1 EGFP range2201058 1 68 787 annotation2201055 1 Suffix range2201055 1 1 22 annotation2201056 1 RNAT.2 range2201056 1 23 66 BBa_K837001_sequence 1 gaattcgcggccgcttctagaggaatacatgttaattatgccatccaggcatacagaagaagttaatatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z