BBa_K842000
1
BBa_K842000
flhB
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Flagellar biosynthetic protein flhB ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P76299
flhB:Quickview ??? EcoliWiki. (n.d.). EcoliWiki. Retrieved October 1, 2012, from http://ecoliwiki.net/colipedia/index.php/flhB:Quickview
Class 2 flagella operon. When transcribed, flhB is one of three flagella genes responsible for the generation of proteins that export flagellin to the flagella apparatus. The flagellin is then directly used in the synthesis of the structures??? basal body. Additionally, flhB directly transcribes the operon flhBAE.
Cloning and Uses
flhB was use generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is directly downstream of the class 1 flagella operons flhD and flhC. It also works in conjunction with fliL and fliH to make up the flagellin export system. This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.
Used for reconstitution of flagella apparatus in E. coli B strains.
false
false
_1101_
0
7936
9
It's complicated
true
None
false
Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
annotation2207279
1
flhB
range2207279
1
1
1149
BBa_K842000_sequence
1
gtgtctgacgagagcgacgacaaaacagaagcccccacacctcaccgactagaaaaagcgcgggaagaggggcaaatcccgcgttcccgtgaactgacctcactgctgattttgctggtgggcgttagtgttatctggtttggcggtgtgtcgctggcccgtcgattgtcgggcatgctctccgctgggctgcattttgatcacagtattatcaatgacccgaatctgatcctcgggcagattattctgctgatcagagaagccatgctggcgctgctgccgctgattagcggcgtggtgctggtggcgctcatttctccggtcatgctgggagggctggtatttagcggcaaatccttgcagccgaagttttccaaactcaacccgctaccgggcattaaacggatgttctcggctcagactggcgcggagttgcttaaagcaattttgaaaaccatcctggttggcagcgtgacggggttttttctctggcatcactggccgcagatgatgcgcttgatggccgagtctccgattaccgccatgggtaatgcgatggatttggtagggctatgcgcactgctggtggtgcttggtgtcattccaatggtgggatttgacgtctttttccaaatattcagccacctgaaaaagctgcgtatgtcacggcaggatattcgtgatgagttcaaacaaagcgaaggtgaccctcatgttaaagggcggatccgtcagatgcagcgagctgctgcacggcgtcggatgatggccgatgtgccgaaagcggatgtcattgtcaataacccgacccactattcggtagcgttgcagtatgacgaaaacaaaatgagcgcaccgaaagtggtcgctaaaggtgcagggctggtcgcgctgcgcattcgtgaaattggcgctgaaaataacgtcccgacgcttgaagcgccgccgctggcgcgagcgctgtatcgacatgcggagattggtcaacaaatcccgggtcaactgtacgccgcggtggcggaagtgctggcctgggtctggcaactgaaacgctggcgtctggctggtggacagcgccctgtacaacctactcatcttccggtgccggaagccctggattttattaacgagaaaccgacccatgagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z