BBa_K842001
1
BBa_K842001
flhD
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Flagellar transcriptional regulator FlhD ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0A8S9
flhD along with flhC forms the master regulation operon for the synthesis of flagella in E. coli. They are responsible for the downstream transcription of the class 2 flagella genes. flhD is directly induced by several separate systems, among these is the two-component quorum sensing response system, qseB and qseC.
Used for reconstitution of flagella apparatus in E. coli B strains.
Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. FlhD is to be used in conjunction with flhC in order to form the transcriptional operon for the production of flagella. This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.
false
false
_1101_
0
7936
9
It's complicated
true
none
false
Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
annotation2210322
1
flhD
range2210322
1
1
360
BBa_K842001_sequence
1
gtgggaataatgcatacctccgagttgctgaaacacatttatgacatcaacttgtcatatttactacttgcacagcgtttgattgttcaggacaaagcgtccgctatgtttcgtctcggcataaatgaagaaatggcgacaacgttagcggcactgactcttccgcaaatggttaagctggcagaaaccaatcaactggtttgtcacttccgttttgacagccaccagacgattactcagttgacgcaagattcccgcgttgacgatctccagcaaattcataccggcatcatgctctcaacacgcttgctgaatgatgttaatcagcctgaagaagcgctgcgcaagaaaagggcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z