BBa_K842006
1
BBa_K842006
cheA
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Chemotaxis protein CheA ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P07363
CheA is a regulatory protein that becomes activated when the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant. The activated cheA self-phosphorylates itself by hydrolyzing ATP in order to gain a phosphate group. CheA then donates its phosphate group to activate cheB or cheA.
Used for researcher control of signaling mechanisms that influence flagella function.
Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
false
false
_1101_
0
7936
9
It's complicated
true
none
false
Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
annotation2210321
1
CheA
range2210321
1
1
1965
BBa_K842006_sequence
1
gtgagcatggatataagcgatttttatcagacattttttgatgaagcggacgaactgttggctgacatggagcagcatttgctggttttgcagccggaagcgccagatgccgaacaattgaatgccatctttcgggctgcccactcgatcaaaggaggggcaggaacttttggcttcagcgttttgcaggaaaccacgcatctgatggaaaacctgctcgatgaagccagacgaggtgagatgcaactcaacaccgacattatcaatctgtttttggaaacgaaggacatcatgcaagaacagctcgacgcttataaacagtcgcaagagccggatgccgccagcttcgattatatctgccaggccttgcgtcaactggcattagaagcgaaaggcgaaacgccatccgcagtgacccgattaagtgtggttgccaaaagtgaaccgcaagatgagcagagtcgcagtcagtcgccgcgacgaattatcctttcgcgcctgaaggccggggaagtcgacctgctggaagaagaactgggacatctgacaacgttaactgacgtggtgaaaggggcggattcgctctcggcaatattaccgggcgacatcgccgaagatgacatcacagcggtactctgttttgtgattgaagccgatcagattacctttgaaacagtagaagtctcgccaaaaatatccaccccaccagtgcttaaactggcagccgaacaagcgccaaccggccgcgtggagcgggaaaaaacgacgcgcagcaatgaatccaccagcatccgtgtagcggtagaaaaggttgatcaattaattaacctcgtcggcgagctggttatcacccagtccatgcttgcccagcgttccagcgaactggacccggttaatcatggtgatttgataaccagcatggggcagttacaacgtaacgcccgtgatttgcaggaatcagtgatgtcgattcgcatgatgccgatggaatatgtttttagtcgctatccccggctggtgcgtgatctggcgggaaaactcggcaagcaggtagaactgacgctggtgggcagttctactgaactcgacaaaagcctgatagaacgcattatcgacccgctgacccacctggtacgcaatagcctcgatcacggtattgaactgccagaaaaacggctcgccgcaggtaaaaacagcgtcggaaatttaattctgtctgccgaacatcagggcggcaacatttgcattgaagtgaccgacgatggggcggggctaaaccgtgagcgaattctggcaaaagcggcctcgcaaggtttgactgtcagcgaaaacatgagcgacgacgaagtcgcgatgctgatatttgcacctggcttctccacggcagagcaggtcaccgacgtctccgggcgcggcgtcggcatggacgtcgttaaacgtaatatccagaagatgggcggtcatgtcgaaatccagtcgaagcagggtactggcactacgatccgcattttactgccgctgacgctggccatcctcgacggcatgtccgtacgcgttgcggatgaagttttcattctgccgctgaatgctgttatggaatcactgcaaccccgtgaagccgatctccatccactggccggcggcgagcgggtgctggaagtgcggggtgaatatctgcccatcgtcgaactgtggaaagtgttcaacgtcgcgggcgcgaaaaccgaagccacccagggaattgtggtgatcttacaaagtggcggtcgccgctacgccttgctggtggatcaattaattggtcaacaccaggttgtggttaaaaaccttgaaagtaactatcgcaaagtccccggcatttctgctgcgaccattcttggcgacggcagcgtggcactgattgttgatgtctccgccttgcaggcgataaaccgcgaacaacgtatggcgaacaccgccgcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z