BBa_K842015 1 BBa_K842015 T7 RBS cheY 2012-10-02T11:00:00Z 2015-05-08T01:13:33Z Chemotaxis protein CheY ??? Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0A2D5 T7 serves at as a promoter that is stimulated by IPTG. RBS is a sequence in DNA located upstream of the start codon. It affects the rate at which the open reading frame is translated. CheY is regulatory protein that revereses the direction of flagella rotation from conter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY is then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella are not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a ???forward??? direction. Flagella that are bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling. Used for researcher control of signaling mechanisms that influence flagella function. http://partsregistry.org/wiki/index.php?title=Part:BBa_K842015 Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella. false false _1101_ 0 7936 9 It's complicated true None false Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto annotation2210106 1 T7 range2210106 1 1 21 annotation2210108 1 CheY range2210108 1 39 428 annotation2210107 1 RBS range2210107 1 22 38 BBa_K842015_sequence 1 aatacgactcactatagggagaataaaggaggtaaataatggcggataaagaacttaaatttttggttgtggatgacttttccaccatgcgacgcatagtgcgtaacctgctgaaagagctgggattcaataatgttgaggaagcggaagatggcgtcgacgctctcaataagttgcaggcaggcggttatggatttgttatctccgactggaacatgcccaatatggatggcctggaattgctgaaaacaattcgtgcggatggcgcgatgtcggcattgccagtgttaatggtgactgcggaagcgaagaaagagaacatcattgctgcggcgcaagcgggggccagtggctatgtggtgaagccatttaccgccgcgacgctggaggaaaaactcaacaaaatctttgagaaactgggcatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z