LOCUS       BBa_K843004             2871 bp    DNA     linear       14-Jun-2024
DEFINITION  T4 lysis device regulated by IPTG
ACCESSION   BBa_K843004
VERSION     BBa_K843004.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             94..750
                     /label=T4 holin
     CDS             78..753
                     /label=BBa_K112805
     CDS             778..1272
                     /label=T4 Endolysin
     CDS             762..1275
                     /label=BBa_K112806
     stem_loop       1295..1338
                     /label=stem_loop
     misc_feature    1284..1363
                     /label=BBa_B0010
     terminator      1284..1363
                     /label=BBa_B0010
     stem_loop       1379..1398
                     /label=T7 TE
     misc_feature    1405..1405
                     /label=stop
     polyA_site      1399..1412
                     /label=polya
     misc_feature    1372..1412
                     /label=BBa_B0012
     terminator      1372..1412
                     /label=BBa_B0012
     promoter        1421..1455
                     /label=BBa_J23116
     CDS             1478..1771
                     /label=T4 antiholin
     CDS             1464..1774
                     /label=BBa_K112807
     stem_loop       1794..1837
                     /label=stem_loop
     misc_feature    1783..1862
                     /label=BBa_B0010
     terminator      1783..1862
                     /label=BBa_B0010
     misc_feature    78..1862
                     /label=BBa_K112808
     misc_feature    1..1862
                     /label=BBa_K843002
     CDS             1871..1958
                     /label=end of LacI coding region (inactive)
     misc_feature    1871..2070
                     /label=BBa_R0010
     misc_feature    1959..1996
                     /label=CAP binding site
     promoter        2007..2012
                     /label=-35
     promoter        2031..2036
                     /label=-10
     misc_feature    2036..2070
                     /label=LacI binding site
     misc_feature    2043..2043
                     /label=start
     promoter        1871..2070
                     /label=BBa_R0010
     misc_feature    2083..2086
                     /label=conserved
     RBS             2079..2090
                     /label=BBa_B0034
     CDS             2100..2807
                     /label=cI lambda
     misc_feature    2808..2840
                     /label=LVA
     misc_feature    2847..2871
                     /label=Help:Barcodes
     CDS             2097..2871
                     /label=BBa_C0051
     misc_feature    1871..2871
                     /label=BBa_K843003
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagagctt aaaaggaggg tctatggcag cacctagaat atcattttcg
      121 ccctctgata ttctatttgg tgttctcgat cgcttgttca aagataacgc taccgggaag
      181 gttcttgctt cccgggtagc tgtcgtaatt cttttgttta taatggcgat tgtttggtat
      241 aggggagata gtttctttga gtactataag caatcaaagt atgaaacata cagtgaaatt
      301 attgaaaagg aaagaactgc acgctttgaa tctgtcgccc tggaacaact ccagatagtt
      361 catatatcat ctgaggcaga ctttagtgcg gtgtattctt tccgccctaa aaacttaaac
      421 tattttgttg atattatagc atacgaagga aaattacctt caacaataag tgaaaaatca
      481 cttggaggat atcctgttga taaaactatg gatgaatata cagttcattt aaatggacgt
      541 cattattatt ccaactcaaa atttgctttt ttaccaacta aaaagcctac tcccgaaata
      601 aactacatgt acagttgtcc atattttaat ttggataata tctatgctgg aacgataacc
      661 atgtactggt atagaaatga tcatataagt aatgaccgcc ttgaatcaat atgtgctcag
      721 gcggccagaa tattaggaag ggctaaataa ttatactaga gatacttagg aggtattatg
      781 aatatatttg aaatgttacg tatagatgaa ggtcttagac ttaaaatcta taaagacaca
      841 gaaggctatt acactattgg catcggtcat ttgcttacaa aaagtccatc acttaatgct
      901 gctaaatctg aattagataa agctattggg cgtaattgca atggtgtaat tacaaaagat
      961 gaggctgaaa aactctttaa tcaggatgtt gatgctgctg ttcgcggaat cctgagaaat
     1021 gctaaattaa aaccggttta tgattctctt gatgcggttc gtcgctgtgc attgattaat
     1081 atggttttcc aaatgggaga aaccggtgtg gcaggattta ctaactcttt acgtatgctt
     1141 caacaaaaac gctgggatga agcagcagtt aacttagcta aaagtagatg gtataatcaa
     1201 acacctaatc gcgcaaaacg agtcattaca acgtttagaa ctggcacttg ggacgcgtat
     1261 aaaaatctat aaagctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1321 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1381 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacagcta gctcagtcct
     1441 agggactatg ctagctacta gaggatagga ggcctttatg gccttaaaag caacagcact
     1501 ttttgccatg ctaggattgt catttgtttt atctccatcg attgaagcga atgtcgatcc
     1561 tcattttgat aaatttatgg aatctggtat taggcacgtt tatatgcttt ttgaaaataa
     1621 aagcgtagaa tcgtctgaac aattctatag ttttatgaga acgacctata aaaatgaccc
     1681 gtgctcttct gattttgaat gtatagagcg aggcgcggag atggcacaat catacgctag
     1741 aattatgaac attaaattgg agactgaatg aaattactag agccaggcat caaataaaac
     1801 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1861 tctactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1921 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1981 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2041 ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga
     2101 gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa
     2161 tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg
     2221 ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata
     2281 acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg
     2341 ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt
     2401 atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct
     2461 ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat
     2521 tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc
     2581 ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca
     2641 tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg
     2701 tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg
     2761 ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg
     2821 aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg c
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