LOCUS BBa_K843007 2729 bp DNA linear 15-Nov-2024 DEFINITION Cell Limiter using quorum sensing ACCESSION BBa_K843007 VERSION BBa_K843007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 misc_feature 44..841 /label=BBa_F1610 misc_feature 1..841 /label=BBa_K843005 misc_feature 850..869 /label=LuxR/HSL promoter 964..971 /label=-10 promoter 938..943 /label=-35 misc_feature 977..977 /label=start misc_feature 979..998 /label=Putative LuxR/HSL misc_feature 850..1000 /label=BBa_R0063 promoter 850..1000 /label=BBa_R0063 misc_feature 1013..1016 /label=conserved RBS 1009..1020 /label=BBa_B0034 misc_feature 1027..1028 /label=prefix misc_feature 1200..1200 /label=T misc_feature 1518..1518 /label=A CDS 1027..1776 /label=luxR misc_feature 1027..1782 /label=BBa_C0062 misc_feature 1783..1807 /label=Help:Barcodes CDS 1027..1782 /label=BBa_C0062 stem_loop 1827..1870 /label=stem_loop misc_feature 1816..1895 /label=BBa_B0010 terminator 1816..1895 /label=BBa_B0010 stem_loop 1911..1930 /label=T7 TE misc_feature 1937..1937 /label=stop polyA_site 1931..1944 /label=polya misc_feature 1904..1944 /label=BBa_B0012 terminator 1904..1944 /label=BBa_B0012 misc_feature 1953..1972 /label=LuxR/HSL promoter 1972..1977 /label=-35 promoter 1994..1999 /label=-10 misc_feature 2005..2005 /label=start misc_feature 1953..2007 /label=BBa_R0062 promoter 1953..2007 /label=BBa_R0062 misc_feature 850..2007 /label=BBa_F2621 misc_feature 2020..2023 /label=conserved RBS 2016..2027 /label=BBa_B0034 CDS 2034..2729 /label=minC CDS 2034..2729 /label=BBa_K299806 misc_feature 850..2729 /label=BBa_K843006 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagaga cctgtacgat cctacaggtg cttatgttaa gtaattgtat tcccagcgat 901 acaatagtgt gacaaaaatc caatttatta gaatcaaatg tcaatccatt accgttttaa 961 tgatatataa cacgcaaaac ttgcgacaaa caataggtaa tactagagaa agaggagaaa 1021 tactagatga aaaacataaa tgccgacgac acatacagaa taattaataa aattaaagct 1081 tgtagaagca ataatgatat taatcaatgc ttatctgata tgactaaaat ggtacattgt 1141 gaatattatt tactcgcgat catttatcct cattctatgg ttaaatctga tatttcaatc 1201 ctagataatt accctaaaaa atggaggcaa tattatgatg acgctaattt aataaaatat 1261 gatcctatag tagattattc taactccaat cattcaccaa ttaattggaa tatatttgaa 1321 aacaatgctg taaataaaaa atctccaaat gtaattaaag aagcgaaaac atcaggtctt 1381 atcactgggt ttagtttccc tattcatacg gctaacaatg gcttcggaat gcttagtttt 1441 gcacattcag aaaaagacaa ctatatagat agtttatttt tacatgcgtg tatgaacata 1501 ccattaattg ttccttctct agttgataat tatcgaaaaa taaatatagc aaataataaa 1561 tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa 1621 agctcttggg atatttcaaa aatattaggt tgcagtgagc gtactgtcac tttccattta 1681 accaatgcgc aaatgaaact caatacaaca aaccgctgcc aaagtatttc taaagcaatt 1741 ttaacaggag caattgattg cccatacttt aaaaattaat aacactgata gtgctagtgt 1801 agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1861 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1921 cgggtgggcc tttctgcgtt tatatactag agacctgtag gatcgtacag gtttacgcaa 1981 gaaaatggtt tgttatagtc gaataaatac tagagaaaga ggagaaatac tagatgtcaa 2041 acacgccaat cgagcttaaa ggcagtagct tcactttatc tgtggttcat ctgcatgagg 2101 cagaacctaa ggttatccat caggcgctgg aagacaaaat cgctcaggcc cccgcatttt 2161 taaaacatgc ccccgttgta ctcaacgtca gtgcactgga agacccggta aactggtcag 2221 cgatgcataa ggcggtttcg gcaaccggtt tgcgggttat tggcgtaagt ggctgcaaag 2281 atgcgcaact taaagccgaa attgaaaaga tggggctgcc tatcctgacg gaaggaaagg 2341 aaaaagcgcc acgtccagct cccacaccgc aggctccagc gcaaaataca acgccggtca 2401 caaaaacgcg tttaatagat accccggtgc gttccggtca gcgtatttat gctccacaat 2461 gtgatctgat tgttacaagc cacgttagcg ctggggccga attgattgcc gatgggaaca 2521 ttcatgtcta tggcatgatg cgcggtcgtg cgctggcagg ggcaagtggt gaccgggaaa 2581 cgcaaatatt ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt gaatactggc 2641 tgagtgatca aatcccagca gaattttatg gcaaagcggc gcgactgcag ttagtcgaaa 2701 acgctttgac cgttcaaccg ttaaattga //