##gff-version 3
##sequence-region BBa_K843007 1 2729
BBa_K843007	.	engineered_region	850	2729	.	+	0	ID=BBa_K843006;Name=BBa_K843006
BBa_K843007	.	ribosome_entry_site	2016	2027	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K843006
BBa_K843007	.	conserved_region	2020	2023	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K843007	.	CDS	2034	2729	.	+	0	ID=BBa_K299806;Name=BBa_K299806;Parent=BBa_K843006
BBa_K843007	.	CDS	2034	2729	.	+	0	ID=annotation2087487;Name=minC;Parent=BBa_K299806
BBa_K843007	.	engineered_region	850	2007	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K843006
BBa_K843007	.	terminator	1904	1944	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K843007	.	stem_loop	1911	1930	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K843007	.	stop_codon	1937	1937	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K843007	.	polyA_site	1931	1944	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K843007	.	engineered_region	1904	1944	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K843007	.	promoter	850	1000	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K843007	.	promoter	938	943	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K843007	.	non_covalent_binding_site	979	998	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K843007	.	non_covalent_binding_site	850	869	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K843007	.	engineered_region	850	1000	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K843007	.	start_codon	977	977	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K843007	.	promoter	964	971	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K843007	.	CDS	1027	1782	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K843007	.	engineered_region	1027	1028	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K843007	.	engineered_tag	1783	1807	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K843007	.	point_mutation	1518	1518	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K843007	.	engineered_region	1027	1782	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K843007	.	point_mutation	1200	1200	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K843007	.	CDS	1027	1776	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K843007	.	terminator	1816	1895	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K843007	.	stem_loop	1827	1870	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K843007	.	engineered_region	1816	1895	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K843007	.	ribosome_entry_site	1009	1020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K843007	.	conserved_region	1013	1016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K843007	.	promoter	1953	2007	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K843007	.	engineered_region	1953	2007	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K843007	.	start_codon	2005	2005	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K843007	.	promoter	1994	1999	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K843007	.	non_covalent_binding_site	1953	1972	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K843007	.	promoter	1972	1977	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K843007	.	engineered_region	1	841	.	+	0	ID=BBa_K843005;Name=BBa_K843005
BBa_K843007	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K843005
BBa_K843007	.	engineered_region	44	841	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_K843005
BBa_K843007	.	CDS	62	679	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_K843007	.	engineered_region	62	679	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K843007	.	tag	641	672	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K843007	.	CDS	62	640	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K843007	.	engineered_tag	680	704	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K843007	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_K843007	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K843007	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K843007	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K843007	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K843007	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_K843007	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K843007	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K843007	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_K843007	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K843007
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgactataatgataaaa
aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac
ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg
tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct
gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct
caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat
tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga
gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag
tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggt
gcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatcca
ttaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgtcaaacacgccaatcgag
cttaaaggcagtagcttcactttatctgtggttcatctgcatgaggcagaacctaaggttatccatcaggcgctggaag
acaaaatcgctcaggcccccgcatttttaaaacatgcccccgttgtactcaacgtcagtgcactggaagacccggtaaa
ctggtcagcgatgcataaggcggtttcggcaaccggtttgcgggttattggcgtaagtggctgcaaagatgcgcaactt
aaagccgaaattgaaaagatggggctgcctatcctgacggaaggaaaggaaaaagcgccacgtccagctcccacaccgc
aggctccagcgcaaaatacaacgccggtcacaaaaacgcgtttaatagataccccggtgcgttccggtcagcgtattta
tgctccacaatgtgatctgattgttacaagccacgttagcgctggggccgaattgattgccgatgggaacattcatgtc
tatggcatgatgcgcggtcgtgcgctggcaggggcaagtggtgaccgggaaacgcaaatattttgtacgaacctgatgg
cggaactggtgtccatcgcaggtgaatactggctgagtgatcaaatcccagcagaattttatggcaaagcggcgcgact
gcagttagtcgaaaacgctttgaccgttcaaccgttaaattga