LOCUS       BBa_K843008             2601 bp    DNA     linear       21-May-2024
DEFINITION  T4 Lysis Device under control of IPTG
ACCESSION   BBa_K843008
VERSION     BBa_K843008.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             94..750
                     /label=T4 holin
     CDS             78..753
                     /label=BBa_K112805
     CDS             778..1272
                     /label=T4 Endolysin
     CDS             762..1275
                     /label=BBa_K112806
     stem_loop       1295..1338
                     /label=stem_loop
     misc_feature    1284..1363
                     /label=BBa_B0010
     terminator      1284..1363
                     /label=BBa_B0010
     stem_loop       1379..1398
                     /label=T7 TE
     misc_feature    1405..1405
                     /label=stop
     polyA_site      1399..1412
                     /label=polya
     misc_feature    1372..1412
                     /label=BBa_B0012
     terminator      1372..1412
                     /label=BBa_B0012
     promoter        1421..1455
                     /label=BBa_J23116
     promoter        1464..1504
                     /label=BBa_K112907
     stem_loop       1524..1567
                     /label=stem_loop
     misc_feature    1513..1592
                     /label=BBa_B0010
     terminator      1513..1592
                     /label=BBa_B0010
     CDS             1601..1688
                     /label=end of LacI coding region (inactive)
     misc_feature    1601..1800
                     /label=BBa_R0010
     misc_feature    1689..1726
                     /label=CAP binding site
     promoter        1737..1742
                     /label=-35
     promoter        1761..1766
                     /label=-10
     misc_feature    1766..1800
                     /label=LacI binding site
     misc_feature    1773..1773
                     /label=start
     promoter        1601..1800
                     /label=BBa_R0010
     misc_feature    1813..1816
                     /label=conserved
     RBS             1809..1820
                     /label=BBa_B0034
     CDS             1830..2537
                     /label=cI lambda
     misc_feature    2538..2570
                     /label=LVA
     misc_feature    2577..2601
                     /label=Help:Barcodes
     CDS             1827..2601
                     /label=BBa_C0051
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagagctt aaaaggaggg tctatggcag cacctagaat atcattttcg
      121 ccctctgata ttctatttgg tgttctcgat cgcttgttca aagataacgc taccgggaag
      181 gttcttgctt cccgggtagc tgtcgtaatt cttttgttta taatggcgat tgtttggtat
      241 aggggagata gtttctttga gtactataag caatcaaagt atgaaacata cagtgaaatt
      301 attgaaaagg aaagaactgc acgctttgaa tctgtcgccc tggaacaact ccagatagtt
      361 catatatcat ctgaggcaga ctttagtgcg gtgtattctt tccgccctaa aaacttaaac
      421 tattttgttg atattatagc atacgaagga aaattacctt caacaataag tgaaaaatca
      481 cttggaggat atcctgttga taaaactatg gatgaatata cagttcattt aaatggacgt
      541 cattattatt ccaactcaaa atttgctttt ttaccaacta aaaagcctac tcccgaaata
      601 aactacatgt acagttgtcc atattttaat ttggataata tctatgctgg aacgataacc
      661 atgtactggt atagaaatga tcatataagt aatgaccgcc ttgaatcaat atgtgctcag
      721 gcggccagaa tattaggaag ggctaaataa ttatactaga gatacttagg aggtattatg
      781 aatatatttg aaatgttacg tatagatgaa ggtcttagac ttaaaatcta taaagacaca
      841 gaaggctatt acactattgg catcggtcat ttgcttacaa aaagtccatc acttaatgct
      901 gctaaatctg aattagataa agctattggg cgtaattgca atggtgtaat tacaaaagat
      961 gaggctgaaa aactctttaa tcaggatgtt gatgctgctg ttcgcggaat cctgagaaat
     1021 gctaaattaa aaccggttta tgattctctt gatgcggttc gtcgctgtgc attgattaat
     1081 atggttttcc aaatgggaga aaccggtgtg gcaggattta ctaactcttt acgtatgctt
     1141 caacaaaaac gctgggatga agcagcagtt aacttagcta aaagtagatg gtataatcaa
     1201 acacctaatc gcgcaaaacg agtcattaca acgtttagaa ctggcacttg ggacgcgtat
     1261 aaaaatctat aaagctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1321 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1381 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacagcta gctcagtcct
     1441 agggactatg ctagctacta gaggatcttt gacagctagc tcagtcctag gtataatgct
     1501 agcgtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1561 gttttatctg ttgtttgtcg gtgaacgctc tctactagag caatacgcaa accgcctctc
     1621 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1681 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     1741 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     1801 tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta acacaagagc
     1861 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct
     1921 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat
     1981 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag
     2041 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg
     2101 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg
     2161 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa
     2221 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga
     2281 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg
     2341 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct
     2401 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc
     2461 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc
     2521 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct
     2581 gatagtgcta gtgtagatcg c
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