BBa_K843008
type: ComponentDefinition
persistent identity: BBa K843008
display id: BBa_K843008
version: 1
was generated by: iGEM to SBOL conversion
description: T4 Lysis Device under control of IPTG
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: All parts used for this device is present in parts registry.
owned by: james
owned by: myers
mutable description: By using this composite part, it is possible to keep living of bacteria under control of IPTG. This part contains a Kill Switch lysis machine designed by iGEM08 UC Berkeley team, with a cI regulated promoter. Also, there is a cI coding region with a LacI regulated promoter. By this way, in a medium containing IPTG, LacI will be inhibited by IPTG. Therefore, LacI regulated promoter will be active and cI protein will be produced. As a result, cI protein will inhibit cI regulated promoter activity. So, kill switch part (T4 Lysis Device) will not work. In contrast, in the absence of IPTG, LacI inhibits its promoter, and so cI will not be produced. Therefore, cI regulated promoter will be active and T4 Lysis Device take bacteria to the lysis. As a result, this composite part has a big potential to be used as a safety device.
discontinued: false
dominant: false
group u list: _1103_
m user id: 0
owner id: 12727
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Since our project has a potential to be used as a biofilter, safety is a big issue. To create a very safe part, we thought that bacteria should be alive only in our system. If any escape from system occurs, bacteria should kill themselves. Thus, we thought to make their lives depending on an easy-found medium compound, IPTG.
star: false
creator: Alişan Kayab??len
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_K112907
component: BBa_J23116
component: BBa_B0012
component: BBa_C0051
component: BBa_B0034
component: BBa_R0051
component: BBa_R0010
component: BBa_K112806
component: BBa_K112805
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_K112805
sequence annotation: BBa_K112806
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23116
sequence annotation: BBa_K112907
sequence annotation: BBa_R0010
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K843008_sequence/1

BBa_B0010
type: Component
persistent identity: component2298714
display id: component2298714
version: 1
top level: BBa_K843008
definition: BBa_B0010
access: public

BBa_K112907
type: Component
persistent identity: component2298713
display id: component2298713
version: 1
top level: BBa_K843008
definition: BBa_K112907
access: public

BBa_J23116
type: Component
persistent identity: component2298712
display id: component2298712
version: 1
top level: BBa_K843008
definition: BBa_J23116
access: public

BBa_B0012
type: Component
persistent identity: component2298708
display id: component2298708
version: 1
top level: BBa_K843008
definition: BBa_B0012
access: public

BBa_C0051
type: Component
persistent identity: component2298728
display id: component2298728
version: 1
top level: BBa_K843008
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2298724
display id: component2298724
version: 1
top level: BBa_K843008
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2298696
display id: component2298696
version: 1
top level: BBa_K843008
definition: cI lam
access: public

BBa_R0010
type: Component
persistent identity: component2298716
display id: component2298716
version: 1
top level: BBa_K843008
definition: LacI
access: public

BBa_B0010
type: Component
persistent identity: component2298706
display id: component2298706
version: 1
top level: BBa_K843008
definition: BBa_B0010
access: public

BBa_K112806
type: Component
persistent identity: component2298705
display id: component2298705
version: 1
top level: BBa_K843008
definition: BBa_K112806
access: public

BBa_K112805
type: Component
persistent identity: component2298703
display id: component2298703
version: 1
top level: BBa_K843008
definition: BBa_K112805
access: public

BBa_B0034
type: Component
persistent identity: component2298701
display id: component2298701
version: 1
top level: BBa_K843008
definition: BBa_B0034
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2298696
display id: annotation2298696
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298696/range2298696/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K843008/annotation2298696/range2298696/1
type: Range
persistent identity: range2298696
display id: range2298696
version: 1
top level: BBa_K843008
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298701
display id: annotation2298701
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298701/range2298701/1
component: BBa_B0034

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type: Range
persistent identity: range2298701
display id: range2298701
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2298703
display id: annotation2298703
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298703/range2298703/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K843008/annotation2298703/range2298703/1
type: Range
persistent identity: range2298703
display id: range2298703
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 78
end: 753
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2298705
display id: annotation2298705
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298705/range2298705/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K843008/annotation2298705/range2298705/1
type: Range
persistent identity: range2298705
display id: range2298705
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 762
end: 1275
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2298706
display id: annotation2298706
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298706/range2298706/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K843008/annotation2298706/range2298706/1
type: Range
persistent identity: range2298706
display id: range2298706
version: 1
top level: BBa_K843008
direction: direction/forward
start: 1284
end: 1363
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2298708
display id: annotation2298708
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298708/range2298708/1
component: BBa_B0012

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type: Range
persistent identity: range2298708
display id: range2298708
version: 1
top level: BBa_K843008
direction: direction/forward
start: 1372
end: 1412
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2298712
display id: annotation2298712
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298712/range2298712/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K843008/annotation2298712/range2298712/1
type: Range
persistent identity: range2298712
display id: range2298712
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 1421
end: 1455
orientation: inline

BBa_K112907
type: SequenceAnnotation
persistent identity: annotation2298713
display id: annotation2298713
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298713/range2298713/1
component: BBa_K112907

https://synbiohub.org/public/igem/BBa_K843008/annotation2298713/range2298713/1
type: Range
persistent identity: range2298713
display id: range2298713
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 1464
end: 1504
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2298714
display id: annotation2298714
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298714/range2298714/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K843008/annotation2298714/range2298714/1
type: Range
persistent identity: range2298714
display id: range2298714
version: 1
top level: BBa_K843008
direction: direction/forward
start: 1513
end: 1592
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2298716
display id: annotation2298716
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298716/range2298716/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K843008/annotation2298716/range2298716/1
type: Range
persistent identity: range2298716
display id: range2298716
version: 1
top level: BBa_K843008
direction: direction/forward
start: 1601
end: 1800
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298724
display id: annotation2298724
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298724/range2298724/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K843008/annotation2298724/range2298724/1
type: Range
persistent identity: range2298724
display id: range2298724
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 1809
end: 1820
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2298728
display id: annotation2298728
version: 1
top level: BBa_K843008
location: https://synbiohub.org/public/igem/BBa_K843008/annotation2298728/range2298728/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K843008/annotation2298728/range2298728/1
type: Range
persistent identity: range2298728
display id: range2298728
version: 1
top level: BBa_K843008
direction: direction/reverse
start: 1827
end: 2601
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_K112907
type: ComponentDefinition
persistent identity: BBa K112907
display id: BBa_K112907
version: 1
was generated by: iGEM to SBOL conversion
description: Pcon
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:20Z
owned by: james
owned by: myers
mutable description: A constituitive promoter
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bing Xia
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112907_sequence/1

https://synbiohub.org/public/igem/BBa_K843008_sequence/1
type: Sequence
persistent identity: BBa K843008 sequence
display id: BBa_K843008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggatctttgacagctagctcagtcctaggtataatgctagcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112907_sequence/1
type: Sequence
persistent identity: BBa K112907 sequence
display id: BBa_K112907_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatctttgacagctagctcagtcctaggtataatgctagcg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z