BBa_K844011
1
BBa_K844011
tRNA Terminator for ''E. coli''
2012-10-02T11:00:00Z
2015-05-08T01:13:33Z
''E. coli'' genomic DNA downstream of the Ala(GCC) tRNA gene.
This tRNA terminator is based around the downstream sequence of the ''E. coli'' Ala(GCC) tRNA gene. It contains the region of 3-6 T???s that is characteristic of tRNA terminators and an additional 10 bp of native sequence. This additional region of native sequence is to ensure RNA termination and to act as a small spacer region between tRNA genes.
It is important to use tRNA specific terminators when expressing tRNA genes, as normal rho-dependent and rho-independent terminators rely on additional RNA sequences after where the gene actually finishes to terminate, and when extra sequences are included on tRNA molecules, their function can be severely affected.
false
false
_1104_
0
9404
9
Not in stock
false
none
false
Kathleen Miller
annotation2210968
1
tRNA terminator
range2210968
1
1
5
annotation2210969
1
Spacer
range2210969
1
6
15
BBa_K844011_sequence
1
aatttccaaccctcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z