LOCUS       BBa_K844015             1159 bp    DNA     linear       01-Jun-2024
DEFINITION  lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP
ACCESSION   BBa_K844015
VERSION     BBa_K844015.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=Start
     misc_feature    230..313
                     /label=Elasticity Domain
     misc_feature    230..238
                     /label=Beta-helix
     misc_feature    239..253
                     /label=Beta-spiral
     misc_feature    260..274
                     /label=Beta-spiral
     misc_feature    275..289
                     /label=Beta-spiral
     misc_feature    298..313
                     /label=Beta-spiral
     misc_feature    314..397
                     /label=Elasticity Domain
     misc_feature    314..323
                     /label=Beta-helix
     misc_feature    324..339
                     /label=Beta-spiral
     misc_feature    343..358
                     /label=Beta-spiral
     misc_feature    359..373
                     /label=Beta-spiral
     misc_feature    382..397
                     /label=Beta-spiral
     misc_feature    398..414
                     /label=Linker Domain
     misc_feature    415..433
                     /label=Strength Domain
     CDS             227..433
                     /label=BBa_K844007
     misc_feature    440..442
                     /label=Start
     CDS             440..1159
                     /label=GFP Coding Region
     CDS             440..1159
                     /label=BBa_K208000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gtggttatgg
      241 tcctggtgca ggtcaacaag gtcctggtag tcaaggtcct ggtagtggtg gtcaacaagg
      301 tcctggtggt caaggtggtt atggtcctgg tgcaggtcaa caaggtcctg gtagtcaagg
      361 tcctggtagt ggtggtcaac aaggtcctgg tggtcaaggt ccttatggtc ctagtgcagc
      421 agcagcagca gcatactaga tggctagcaa aggagaagaa cttttcactg gagttgtccc
      481 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      541 tgaaggtgat gctacatacg gaaagcttac ccttaaattt atttgcacta ctggaaaact
      601 acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcccg
      661 ttatccggat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
      721 acaggaacgc actatatctt tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa
      781 gtttgaaggt gatacccttg ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga
      841 tggaaacatt ctcggacaca aactcgagta caactataac tcacacaatg tatacatcac
      901 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acattgaaga
      961 tggatccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1021 ccttttacca gacaaccatt acctgtcgac acaatctgcc ctttcgaaag atcccaacga
     1081 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat
     1141 ggatgagctc tacaaataa
//