BBa_K208000 1 BBa_K208000 GFP 2009-10-07T11:00:00Z 2015-05-08T01:11:24Z plasmid GFP false false _310_ 0 2425 303 It's complicated true Mutate PstI sites false Elisabeth Linton annotation2062217 1 GFP Coding Region range2062217 1 1 720 annotation2062216 1 Start range2062216 1 1 3 BBa_K844015 1 PatgF1GFP lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP 2012-10-01T11:00:00Z 2016-02-10T11:29:32Z Assembly standard #23 assembly of basic parts This part is a spider silk 1x "F" subunit with GFP fused to its C-terminus. This generator is lactose/ITPG inducible. false false _1104_ 4206 14072 9 It's complicated true none false Brian Smith component2211555 1 BBa_K844007 component2211558 1 BBa_K208000 component2211554 1 BBa_K208010 annotation2211555 1 BBa_K844007 range2211555 1 227 433 annotation2211554 1 BBa_K208010 range2211554 1 1 220 annotation2211558 1 BBa_K208000 range2211558 1 440 1159 BBa_K208010 1 BBa_K208010 Lac Promoter (BBa_R0010) and RBS (B0034) 2009-10-11T11:00:00Z 2015-05-08T01:11:24Z synthetic Released HQ 2013 lac + rbs false false _310_ 0 3473 9 In stock true none false Elisabeth Linton annotation2034625 1 BBa_B0034 range2034625 1 209 220 annotation2034626 1 BBa_R0010 range2034626 1 1 200 BBa_K844007 1 BBa_K844007 Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized) with Met (ATG) added 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844003 Released HQ 2013 Spider silk subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon. false false _1104_ 0 10131 9 In stock false none false Charles Barentine annotation2212472 1 Beta-spiral range2212472 1 72 87 annotation2212478 1 Beta-spiral range2212478 1 156 171 annotation2212467 1 Elasticity Domain range2212467 1 4 87 annotation2212476 1 Beta-spiral range2212476 1 117 132 annotation2212479 1 Linker Domain range2212479 1 172 188 annotation2212474 1 Beta-helix range2212474 1 88 97 annotation2212471 1 Beta-spiral range2212471 1 49 63 annotation2212470 1 Beta-spiral range2212470 1 34 48 annotation2212475 1 Beta-spiral range2212475 1 98 113 annotation2212469 1 Beta-spiral range2212469 1 13 27 annotation2212466 1 Start range2212466 1 1 3 annotation2212473 1 Elasticity Domain range2212473 1 88 171 annotation2212477 1 Beta-spiral range2212477 1 133 147 annotation2212480 1 Strength Domain range2212480 1 189 207 annotation2212468 1 Beta-helix range2212468 1 4 12 BBa_K844007_sequence 1 atgggtggttatggtcctggtgcaggtcaacaaggtcctggtagtcaaggtcctggtagtggtggtcaacaaggtcctggtggtcaaggtggttatggtcctggtgcaggtcaacaaggtcctggtagtcaaggtcctggtagtggtggtcaacaaggtcctggtggtcaaggtccttatggtcctagtgcagcagcagcagcagca BBa_K208000_sequence 1 atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa BBa_K208010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_K844015_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggtggttatggtcctggtgcaggtcaacaaggtcctggtagtcaaggtcctggtagtggtggtcaacaaggtcctggtggtcaaggtggttatggtcctggtgcaggtcaacaaggtcctggtagtcaaggtcctggtagtggtggtcaacaaggtcctggtggtcaaggtccttatggtcctagtgcagcagcagcagcagcatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z