Spider Silk Generator - 10x "F" Silk Construct with His Tag
Created with standard assembly (standard #23)
Lac Promoter (BBa_R0010) and RBS (B0034)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
Constructed through oligonucleotide annealing
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized)
Artificially designed based on BBa_K844002 and DNA was synthesized.
Spider silk (1x) 2E subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain.
Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized) with Met (ATG) added
Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844003
Spider silk subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
Conversion of the iGEM parts registry to SBOL2.1