LOCUS       BBa_K845000             1242 bp    DNA     linear       20-Apr-2024
DEFINITION  iGEM 2012 Grenoble Team proposes a new design and application to 
            the pBAD promoter(paraBAD).
ACCESSION   BBa_K845000
VERSION     BBa_K845000.1  
FEATURES             Location/Qualifiers
     promoter        1..499
                     /label=paraBAD
     misc_feature    367..383
                     /label=AraC activator
     misc_feature    346..362
                     /label=AraC dual activity (inhibitor/activator)
     misc_feature    314..335
                     /label=CRP activator
     misc_feature    293..309
                     /label=AraC inhibitor
     misc_feature    272..288
                     /label=AraC inhibitor
     misc_feature    135..151
                     /label=AraC inhibitor
     misc_feature    418..499
                     /label=AraB 82 first bp
     promoter        1..499
                     /label=BBa_K845001
     RBS             500..525
                     /label=Strong RBS 
     CDS             525..1242
                     /label=GFPmut2
     misc_feature    500..1242
                     /label=BBa_K845002
ORIGIN
        1 caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc agcaggggat
       61 cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa accaattgtc
      121 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac
      181 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
      241 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
      301 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
      361 ttttatcgca actctctact gtttctccat acccgttttt ttggatggag tgaaacgatg
      421 gcgattgcaa ttggcctcga ttttggcagt gattctgtgc gagctttggc ggtggactgc
      481 gctaccggtg aagctcgagt ctagatttaa gaaggagata tacatatgtc taaaggtgaa
      541 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac
      601 aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa
      661 tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcgcg
      721 tatggtcttc aatgttttgc tagataccca gatcatatga aacaacatga ctttttcaag
      781 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac
      841 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta
      901 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat
      961 aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc
     1021 aaaattagac acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat
     1081 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct
     1141 gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact
     1201 gctgctggta ttacccatgg tatggatgaa ttgtacaaat aa
//