LOCUS BBa_K845009 3827 bp DNA linear 26-Dec-2024 DEFINITION paraBAD_gfp_cyaA ACCESSION BBa_K845009 VERSION BBa_K845009.1 FEATURES Location/Qualifiers promoter 1..499 /label=paraBAD misc_feature 367..383 /label=AraC activator misc_feature 346..362 /label=AraC dual activity (inhibitor/activator) misc_feature 314..335 /label=CRP activator misc_feature 293..309 /label=AraC inhibitor misc_feature 272..288 /label=AraC inhibitor misc_feature 135..151 /label=AraC inhibitor misc_feature 418..499 /label=AraB 82 first bp promoter 1..499 /label=BBa_K845001 RBS 500..525 /label=Strong RBS CDS 525..1242 /label=GFPmut2 misc_feature 500..1242 /label=BBa_K845002 misc_feature 1..1242 /label=BBa_K845000 RBS 1251..1280 /label=WT RBS CDS 1280..3827 /label=cyaA CDS 1251..3827 /label=BBa_K845008 ORIGIN 1 caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc agcaggggat 61 cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa accaattgtc 121 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac 181 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa 241 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg 301 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct 361 ttttatcgca actctctact gtttctccat acccgttttt ttggatggag tgaaacgatg 421 gcgattgcaa ttggcctcga ttttggcagt gattctgtgc gagctttggc ggtggactgc 481 gctaccggtg aagctcgagt ctagatttaa gaaggagata tacatatgtc taaaggtgaa 541 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac 601 aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa 661 tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcgcg 721 tatggtcttc aatgttttgc tagataccca gatcatatga aacaacatga ctttttcaag 781 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac 841 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta 901 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat 961 aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc 1021 aaaattagac acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat 1081 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct 1141 gccttatcca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact 1201 gctgctggta ttacccatgg tatggatgaa ttgtacaaat aatactagag catgacgggt 1261 agcaaatcag gcgatacgtc ttgtacctct atattgagac tctgaaacag agactggatg 1321 ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg 1381 tctacagtct actgccgaca ttgttgcact atcaccatcc gctaatgccg ggttaccttg 1441 atggtaacgt tcccaaaggc atttgccttt acacgcctga tgaaactcaa cgccactacc 1501 tgaacgagct tgaactgtat cgtggaatgt cagtacagga tccgccgaaa ggtgagcttc 1561 caattactgg tgtatacacc atgggcagca cctcgtccgt agggcaaagt tgttcctctg 1621 acctggatat ctgggtctgt catcaatcct ggctcgatag cgaagagcgc caattgctac 1681 aacgtaaatg tagcctgctg gaaaactggg ccgcctcgct gggtgtggaa gtcagcttct 1741 tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt 1801 gtggctccac ccagcatata ctgctgcttg acgaatttta tcgtaccgcc gtgcgtctcg 1861 ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga agaagagcat tacgacgact 1921 atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg 1981 gcttaagctc gctttctgct gaagagtact ttggtgccag cctttggcag ctctacaaga 2041 gtatcgattc cccatacaaa gcggtactga aaacactgct gctggaagcc tattcctggg 2101 aatacccgaa cccacgtctg ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga 2161 ttgtatcgtt tggtctcgat ccatactgca tgatgctgga gcgtgttact gaatacctga 2221 cggcgattga agattttacc cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt 2281 gcgaaaagct cagccgtgaa cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt 2341 tagtgagcga gtggggttgg gacgaagctc gtctggcaat gctcgataac cgcgctaact 2401 ggaagattga tcaggtgcgt gaggcgcaca acgagttgct cgacgcgatg atgcagagct 2461 accgtaatct gatccgcttt gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg 2521 atatcggcgt gctgacgcgt aagctgtatg ccgcgtttga agcattacca ggtaaagtga 2581 cgctggtaaa cccgcagatt tcacccgatc tctcggaacc gaatctgacc tttatttatg 2641 tgccgccggg ccgggctaac cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg 2701 agtcgatcat cagccatcag ccgctggaat ataaccgtta cctgaataaa ctggtggcgt 2761 gggcatggtt taacggcctg ctgacctcgc gcacccgttt gtatattaaa ggtaacggca 2821 ttgtcgattt gcctaagttg caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc 2881 gcttacctgc accgacaccg aaggcgctct acagcccgtg tgagatccgc catctggcga 2941 ttatcgttaa cctggaatat gacccgacag cggcgttccg caatcaggtg gtgcatttcg 3001 atttccgtaa gctggatgtc ttcagctttg gcgagaatca aaattgcctg gtaggtagcg 3061 ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac gctgcacttc aacggcgagc 3121 aatcgatgat cgaagccctg aaaactattc tcggcaaaat gcatcaggac gccgcaccgc 3181 cagatagcgt ggaagtcttc tgttatagcc agcatctgcg cggcttaatt cgtactcgcg 3241 tgcagcaact ggtttctgag tgtattgaat tgcgtctttc cagcacccgc caggaaaccg 3301 ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg gttgttcttc gaacgcctga 3361 atgtatcggt acagaaactg gaaaacgcca tcgagtttta tggcgcgatt tcgcataaca 3421 aactgcacgg cctgtcagtg caggttgaaa ccaatcacgt caaattaccg gcggtggtgg 3481 acggctttgc cagcgaaggg atcatccagt tctttttcga agaaacgcaa gacgagaatg 3541 gctttaatat ctacattctc gacgaaagca accgggttga ggtatatcac cactgcgaag 3601 gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta ctcgtcatcg catgaccgtt 3661 ttacctacgg ctcaagcttc atcaacttca acctgccgca gttctatcag attgtgaagg 3721 ttgatggtcg tgaacaggtg attccgttcc gcacaaaatc tatcggtaac atgccgcctg 3781 ccaatcagga tcacgatacg ccgctattac agcaatattt ttcgtga //