##gff-version 3
##sequence-region BBa_K845009 1 3827
BBa_K845009	.	engineered_region	1	1242	.	+	0	ID=BBa_K845000;Name=BBa_K845000
BBa_K845009	.	promoter	1	499	.	+	0	ID=BBa_K845001;Name=BBa_K845001;Parent=BBa_K845000
BBa_K845009	.	non_covalent_binding_site	367	383	.	+	0	ID=annotation2199721;Name=AraC activator;Parent=BBa_K845001
BBa_K845009	.	non_covalent_binding_site	293	309	.	+	0	ID=annotation2199732;Name=AraC inhibitor;Parent=BBa_K845001
BBa_K845009	.	non_covalent_binding_site	314	335	.	+	0	ID=annotation2199725;Name=CRP activator;Parent=BBa_K845001
BBa_K845009	.	non_covalent_binding_site	272	288	.	+	0	ID=annotation2199737;Name=AraC inhibitor;Parent=BBa_K845001
BBa_K845009	.	promoter	1	499	.	+	0	ID=annotation2199720;Name=paraBAD;Parent=BBa_K845001
BBa_K845009	.	non_covalent_binding_site	346	362	.	+	0	ID=annotation2199723;Name=AraC dual activity (inhibitor/activator);Parent=BBa_K845001
BBa_K845009	.	non_covalent_binding_site	135	151	.	+	0	ID=annotation2199738;Name=AraC inhibitor;Parent=BBa_K845001
BBa_K845009	.	sequence_feature	418	499	.	+	0	ID=annotation2203948;Name=AraB 82 first bp;Parent=BBa_K845001
BBa_K845009	.	engineered_region	500	1242	.	+	0	ID=BBa_K845002;Name=BBa_K845002;Parent=BBa_K845000
BBa_K845009	.	ribosome_entry_site	500	525	.	+	0	ID=annotation2203924;Name=Strong RBS ;Parent=BBa_K845002
BBa_K845009	.	CDS	525	1242	.	+	0	ID=annotation2203925;Name=GFPmut2;Parent=BBa_K845002
BBa_K845009	.	CDS	1251	3827	.	+	0	ID=BBa_K845008;Name=BBa_K845008
BBa_K845009	.	CDS	1280	3827	.	+	0	ID=annotation2203950;Name=cyaA;Parent=BBa_K845008
BBa_K845009	.	ribosome_entry_site	1251	1280	.	+	0	ID=annotation2203949;Name=WT RBS;Parent=BBa_K845008
>BBa_K845009
caatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccat
acttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttac
tggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaa
aaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgc
catagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccg
tttttttggatggagtgaaacgatggcgattgcaattggcctcgattttggcagtgattctgtgcgagctttggcggtg
gactgcgctaccggtgaagctcgagtctagatttaagaaggagatatacatatgtctaaaggtgaagaattattcactg
gtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtga
tgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcact
actttcgcgtatggtcttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgc
cagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttga
aggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattg
gaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaatta
gacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtctt
gttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttg
ttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataatactagagcatgacgggtagca
aatcaggcgatacgtcttgtacctctatattgagactctgaaacagagactggatgccataaatcaattgcgtgtggat
cgcgcgcttgctgctatggggcctgcattccaacaggtctacagtctactgccgacattgttgcactatcaccatccgc
taatgccgggttaccttgatggtaacgttcccaaaggcatttgcctttacacgcctgatgaaactcaacgccactacct
gaacgagcttgaactgtatcgtggaatgtcagtacaggatccgccgaaaggtgagcttccaattactggtgtatacacc
atgggcagcacctcgtccgtagggcaaagttgttcctctgacctggatatctgggtctgtcatcaatcctggctcgata
gcgaagagcgccaattgctacaacgtaaatgtagcctgctggaaaactgggccgcctcgctgggtgtggaagtcagctt
cttcctgattgatgaaaaccgcttccgtcataatgaaagcggcagcctggggggcgaagattgtggctccacccagcat
atactgctgcttgacgaattttatcgtaccgccgtgcgtctcgccggtaagcgtattctgtggaatatggtgccgtgcg
acgaagaagagcattacgacgactatgtgatgacgctttacgcgcagggcgtgctgacgccaaatgaatggctggatct
cggtggcttaagctcgctttctgctgaagagtactttggtgccagcctttggcagctctacaagagtatcgattcccca
tacaaagcggtactgaaaacactgctgctggaagcctattcctgggaatacccgaacccacgtctgctggcgaaagata
tcaaacagcgtttgcacgacggcgagattgtatcgtttggtctcgatccatactgcatgatgctggagcgtgttactga
atacctgacggcgattgaagattttacccgtctggatttagtacgtcgctgcttctatttaaaagtgtgcgaaaagctc
agccgtgaacgcgcctgcgtaggctggcgtcgcgcagtgttgagccagttagtgagcgagtggggttgggacgaagctc
gtctggcaatgctcgataaccgcgctaactggaagattgatcaggtgcgtgaggcgcacaacgagttgctcgacgcgat
gatgcagagctaccgtaatctgatccgctttgcgcgtcgcaataaccttagcgtctccgccagtccgcaggatatcggc
gtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccg
atctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccg
cgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggca
tggtttaacggcctgctgacctcgcgcacccgtttgtatattaaaggtaacggcattgtcgatttgcctaagttgcagg
agatggtcgccgacgtgtcgcaccatttcccgctgcgcttacctgcaccgacaccgaaggcgctctacagcccgtgtga
gatccgccatctggcgattatcgttaacctggaatatgacccgacagcggcgttccgcaatcaggtggtgcatttcgat
ttccgtaagctggatgtcttcagctttggcgagaatcaaaattgcctggtaggtagcgttgacctgctgtaccgcaact
cgtggaacgaagtgcgtacgctgcacttcaacggcgagcaatcgatgatcgaagccctgaaaactattctcggcaaaat
gcatcaggacgccgcaccgccagatagcgtggaagtcttctgttatagccagcatctgcgcggcttaattcgtactcgc
gtgcagcaactggtttctgagtgtattgaattgcgtctttccagcacccgccaggaaaccgggcgtttcaaggcgctgc
gcgtttctggtcaaacctgggggttgttcttcgaacgcctgaatgtatcggtacagaaactggaaaacgccatcgagtt
ttatggcgcgatttcgcataacaaactgcacggcctgtcagtgcaggttgaaaccaatcacgtcaaattaccggcggtg
gtggacggctttgccagcgaagggatcatccagttctttttcgaagaaacgcaagacgagaatggctttaatatctaca
ttctcgacgaaagcaaccgggttgaggtatatcaccactgcgaaggcagcaaagaggagctggtacgtgacgtcagtcg
cttctactcgtcatcgcatgaccgttttacctacggctcaagcttcatcaacttcaacctgccgcagttctatcagatt
gtgaaggttgatggtcgtgaacaggtgattccgttccgcacaaaatctatcggtaacatgccgcctgccaatcaggatc
acgatacgccgctattacagcaatatttttcgtga