LOCUS       BBa_K847101             1104 bp    DNA     linear       22-Dec-2024
DEFINITION  Engineered FliC with insertable expression region
ACCESSION   BBa_K847101
VERSION     BBa_K847101.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    65..592
                     /label=Conserved FliC N terminus
     misc_feature    593..671
                     /label=Multiple Cloning Site
     misc_feature    672..967
                     /label=Conserved FliC C terminus
     CDS             65..967
                     /label=BBa_K847102
     stem_loop       987..1030
                     /label=stem_loop
     misc_feature    976..1055
                     /label=BBa_B0010
     terminator      976..1055
                     /label=BBa_B0010
     stem_loop       1071..1090
                     /label=T7 TE
     misc_feature    1097..1097
                     /label=stop
     polyA_site      1091..1104
                     /label=polya
     misc_feature    1064..1104
                     /label=BBa_B0012
     terminator      1064..1104
                     /label=BBa_B0012
     terminator      976..1104
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggca caagtcatta ataccaacag cctctcgctg atcactcaaa ataatatcaa
      121 caagaaccag tctgcgctgt cgagttctat cgagcgtctg tcctctggct tgcgtattaa
      181 cagcgcgaag gatgacgcag cgggtcaggc gattgctaac cgtttcacct ctaacattaa
      241 aggcctgact caggcggccc gtaacgccaa cgacggtatc tccgttgcgc agaccaccga
      301 aggcgcgctg tccgaaatca acaacaactt acagcgtgtg cgtgaactga cggtacaggc
      361 cactaccggt actaactctg agtctgatct gtcctctatc caggacgaaa ttaaatcccg
      421 tctggatgaa attgaccgcg tatctggtca gacccagttc aacggcgtga acgtgctggc
      481 aaaaaatggc tccatgaaaa tccaggttgg cgcaaatgat aaccagacta tcactatcga
      541 tctgaagcag attgatgcta aaactcttgg ccttgatggt tttagcgtta aaggatccgg
      601 cgggaccggc agatctggca agcttggcca tatgggccga tcgggcgcat gcggccccgg
      661 gggggtcgac gctgttgcaa atggtaaaac caccgatccg ctgaaagcgc tggacgatgc
      721 tatcgcatct gtagacaaat tccgttcttc cctcggtgcg gtgcaaaacc gtctggattc
      781 cgcggttacc aacctgaaca acaccactac caacctgtct gaagcgcagt cccgtattca
      841 ggacgccgac tatgcgaccg aagtgtccaa tatgtcgaaa gcgcagatca tccagcaggc
      901 cggtaactcc gtgttggcaa aagctaacca ggtaccgcag caggttctgt ctctgctcca
      961 gggttaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1021 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1081 cgggtgggcc tttctgcgtt tata
//