BBa_K848003 1 BBa_K848003 luxI-lacI-mCherry under lasB /tetO hybrid promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:34Z BBa_S03816 lasB promoter region is activated by LasR and PAI. The TetO promoter is constitutively ON and repressed by TetR. TetR repression is stopped by the addition of tetracycline. This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting as a pLuxR promoter activator when bound to LuxR protein (BBa_K329007) LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription. false false _1108_ 0 13691 9 No part sequence true ... false Antoine Ribeiro igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z