BBa_K848004 1 BBa_K848004 luxI-lacI-mCherry under LsrA/cI/tetO hybrid promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:34Z BBa_S03816 The LsrA promoter is repressed by LsrR protein (BBa_K848000). Repression is inhibited by the presence of AI-2 signal molecule phosphorilated by LsrK protein (BBa_K848001) . The cI promoter is repressed by the cI repressor (BBa_C0051) The TetO promoter is repressed by TetR. TetR repression is inhibited by the addition of tetracycline. This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting a pLuxR promoter activator when bound to LuxR protein (BBa_K329007) LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription. false false _1108_ 0 13691 9 No part sequence false ... false Antoine Ribeiro igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z