BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K855004 1 BBa_K855004 pvdQ gene form pseudomonas aeruginosa PAO1 with his tag added before stop condon 2012-09-25T11:00:00Z 2015-05-08T01:13:34Z pseudomonas aeruginosa PAO1 pvdQ gene codes for an acylase that can degrade long-chain Nacylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL. false false _1115_ 0 13531 9 Not in stock false pvdQ gene contains a PstI site. false Ma Tsz Shan annotation2200235 1 His Tag range2200235 1 2287 2304 annotation2200185 1 Alpha Chain range2200185 1 70 579 annotation2200186 1 Spacer Chain range2200186 1 580 648 annotation2200188 1 Beta Chain range2200188 1 649 2286 annotation2200243 1 Stop condon range2200243 1 2305 2307 annotation2200184 1 Signal peptide range2200184 1 1 69 BBa_K855000 1 BBa_K855000 pvdQ-B0015 2012-09-19T11:00:00Z 2015-05-08T01:13:34Z Pseudomonas aeruginosa PAO1 This part consists of the gene pvdQ isolated from Pseudomonas areuginosa. pvdQ is an acylase that is capable of degrading long-chain acyl homoserine lactone (AHL) molecules with 6 to 12 carbons. false false _1115_ 0 13414 9 It's complicated false The pvdQ gene consists of a EcoRI-like site. This means that although the exact EcoRI sequence is not present , the EcoRI restriction enzyme is still capable of cutting pvdQ once. Thereby, a short digestion time is needed to lessen this star activity. false Akansha Mahavir Shah, Ma Tsz Shan, Nelson Chu component2200278 1 BBa_B0015 component2200271 1 BBa_K855004 annotation2200271 1 BBa_K855004 range2200271 1 1 2307 annotation2200278 1 BBa_B0015 range2200278 1 2316 2444 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K855004_sequence 1 atggggatgcgtaccgtactgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccgcggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggctacgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggcagcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgctgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcctccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgcctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcggagaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcagcaacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaacggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgccggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcgcctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcgatcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtggtctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtactgcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccctgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccggaactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgcctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttcgtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctgcagggcttctcgcccctggtcagccaggagaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcgaggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccagggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggcttcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcccctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcggcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgcatcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggtggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcccagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgttcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K855000_sequence 1 atggggatgcgtaccgtactgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccgcggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggctacgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggcagcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgctgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcctccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgcctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcggagaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcagcaacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaacggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgccggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcgcctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcgatcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtggtctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtactgcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccctgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccggaactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgcctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttcgtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctgcagggcttctcgcccctggtcagccaggagaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcgaggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccagggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggcttcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcccctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcggcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgcatcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggtggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcccagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgttcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z