LOCUS       BBa_K855003             2426 bp    DNA     linear       22-Nov-2024
DEFINITION  coding sequence for pvdQ with histag added to the C-terminal 
            followed by stop codon
ACCESSION   BBa_K855003
VERSION     BBa_K855003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..69
                     /label=Signal peptide
     CDS             70..579
                     /label=Alpha Chain
     misc_feature    580..648
                     /label=Spacer Chain
     CDS             649..2286
                     /label=Beta Chain
     misc_feature    2287..2304
                     /label=His Tag
     misc_feature    2305..2307
                     /label=Stop condon
     CDS             1..2289
                     /label=BBa_K855004
     stem_loop       2309..2352
                     /label=stem_loop
     misc_feature    2298..2377
                     /label=BBa_B0010
     terminator      2298..2377
                     /label=BBa_B0010
     stem_loop       2393..2412
                     /label=T7 TE
     misc_feature    2419..2419
                     /label=stop
     polyA_site      2413..2426
                     /label=polya
     misc_feature    2386..2426
                     /label=BBa_B0012
     terminator      2386..2426
                     /label=BBa_B0012
     terminator      2298..2426
                     /label=BBa_B0015
ORIGIN
        1 atggggatgc gtaccgtact gaccggcctg gccggcatgc tgttgggttc gatgatgccg
       61 gtccaggccg atatgccgcg gccgaccggg ctggccgcgg atatccgctg gaccgcctat
      121 ggcgtgccgc acatccgggc caaggatgag cgcggcctgg gctatggcat cggctacgcc
      181 tacgcgcgcg acaacgcctg cctgctggcc gaggagatcg tcaccgcgcg cggcgagcgg
      241 gcgcgctatt tcggcagcga gggcaagtcg tcggccgagc tggacaacct gccgtccgac
      301 atcttctacg cctggctcaa ccaacccgag gcgctgcaag ccttctggca ggcgcagacg
      361 cccgcggtac gccagttgct cgaaggctac gccgccggtt tcaaccgctt cctccgcgag
      421 gccgacggca agaccaccag ttgccttggc cagccctggc tgcgggccat cgcgaccgat
      481 gacctgctgc gcctgacccg gcgcctgctg gtcgaaggcg gggtcggcca gttcgccgac
      541 gcgctggtgg ccgccgcgcc gcccggagcg gagaaggtcg ccttgagcgg cgagcaggcg
      601 ttccaggtcg ccgagcagcg gcgccagcgc ttccgcctgg agcgcggcag caacgccatt
      661 gccgttggca gcgaacgttc ggcggacggc aagggcatgc tcctggccaa cccgcacttc
      721 ccctggaacg gcgcgatgcg tttctaccag atgcacctga ccattcccgg ccggctcgac
      781 gtgatggggg cctcgctgcc cggcctgccg gtggtcaaca tcggcttcag ccgccacctg
      841 gcctggaccc acacggtgga tacctccagc cacttcaccc tgtatcgcct ggcgctggac
      901 ccgaaggacc cgcggcgcta cctggtcgac ggtcgttcgc tgccgctgga ggagaagtcc
      961 gtcgcgatcg aggtgcgcgg cgccgacggc aagctgtcgc gcgtcgagca caaggtctac
     1021 cagtcgatct acggcccgct ggtggtctgg cccggcaagc tggactggaa ccgcagcgag
     1081 gcctatgcgc tgcgtgacgc caacctggag aacacccggg tactgcaaca gtggtactcg
     1141 atcaaccagg ccagcgacgt cgccgacctg cgccggcgcg tcgaggcgct acaggggatc
     1201 ccctgggtca acaccctggc cgcggacgag cagggcaacg ccctgtacat gaaccagtcg
     1261 gtggtgccct acctgaagcc ggaactgatt cccgcctgcg ccattccgca actggtcgcc
     1321 gaaggcctgc cggccctcca ggggcaggac agccgctgtg cctggagtcg cgacccggcc
     1381 gcggcccagg ctggcatcac cccggcggcg caactgccgg tgctgttgcg cagggacttc
     1441 gtgcagaact ccaacgacag cgcctggctg accaacccgg cgagcccgct gcagggcttc
     1501 tcgcccctgg tcagccagga gaagcccatc ggtccgcggg cgcgctacgc cctgagccgg
     1561 ctacagggca agcagccgct ggaggcgaag acgctcgagg agatggtcac cgccaaccat
     1621 gtcttcagcg ccgaccaggt gctgccggac ctgctccgcc tgtgccgcga caaccagggc
     1681 gagaagtccc ttgcccgcgc ctgcgcggcc ctggcgcagt gggaccgtgg cgccaacctc
     1741 gacagcggca gcggcttcgt ctacttccag cgcttcatgc aacgcttcgc cgaactcgac
     1801 ggcgcgtgga aggaaccgtt cgatgcgcaa cgtcccctgg atacgccgca aggcatcgcc
     1861 ctcgaccggc cgcaggtggc gacccaggtg cgccaggcgc tggcggacgc ggcggcggag
     1921 gtggagaaga gcgggattcc cgacggcgcg cgctggggcg acctgcaagt gagcacccgt
     1981 ggccaggaac gcatcgcgat tcccggcggc gatggccatt tcggggtcta caacgcgatc
     2041 cagagcgtcc gcaagggcga ccacctggag gtggtcggcg gcactagcta catccagctg
     2101 gtgaccttcc ccgaggaagg gcccaaggct cgcgggttgc tggctttctc ccagtccagc
     2161 gatccgcgct cgccgcacta ccgcgaccag accgagctgt tttcccgcca gcaatggcag
     2221 accttgccgt tcagcgacag gcagatcgac gccgacccgc aactgcaacg gctaagcatt
     2281 cgcgaatgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2341 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2401 ttcgggtggg cctttctgcg tttata
//