BBa_K855005 1 BBa_K855005 pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site 2012-10-01T11:00:00Z 2015-05-08T01:13:34Z pseudomonas aeruginosa PAO1 pvdQ gene codes for an acylase that can degrade long-chain N-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL. A site-directed mutagenesis at 1491 bp is introduced to the leucine codon, CTG > CTC, to remove the internal illegal PstI site. After the mutagenesis, the biobrick will be compatible with the standard assembly and the pvdQ gene expressed will still be functional. false false _1115_ 0 13531 9 It's complicated false A his tag is added at the C-terminal of the gene sequence to help the detection of the expressed protein. false Ma Tsz Shan annotation2207619 1 his tag range2207619 1 2287 2304 annotation2207600 1 Signal peptide range2207600 1 1 69 annotation2207618 1 stop range2207618 1 2305 2307 annotation2207613 1 pvdQ gene range2207613 1 70 2286 annotation2207616 1 silent mutation "G > C" to remove PstI site range2207616 1 1491 1491 BBa_K855005_sequence 1 atggggatgcgtaccgtactgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccgcggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggctacgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggcagcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgctgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcctccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgcctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcggagaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcagcaacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaacggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgccggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcgcctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcgatcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtggtctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtactgcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccctgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccggaactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgcctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttcgtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctccagggcttctcgcccctggtcagccaggagaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcgaggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccagggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggcttcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcccctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcggcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgcatcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggtggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcccagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgttcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z