LOCUS BBa_K855006 2307 bp DNA linear 31-Oct-2024 DEFINITION pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site ACCESSION BBa_K855006 VERSION BBa_K855006.1 FEATURES Location/Qualifiers misc_feature 1..69 /label=signal peptide CDS 70..2286 /label=pvdQ gene misc_feature 1494..1494 /label=silent mutation "G > A" to remove the PstI site misc_feature 2305..2307 /label=stop misc_feature 2287..2304 /label=his tag ORIGIN 1 atggggatgc gtaccgtact gaccggcctg gccggcatgc tgttgggttc gatgatgccg 61 gtccaggccg atatgccgcg gccgaccggg ctggccgcgg atatccgctg gaccgcctat 121 ggcgtgccgc acatccgggc caaggatgag cgcggcctgg gctatggcat cggctacgcc 181 tacgcgcgcg acaacgcctg cctgctggcc gaggagatcg tcaccgcgcg cggcgagcgg 241 gcgcgctatt tcggcagcga gggcaagtcg tcggccgagc tggacaacct gccgtccgac 301 atcttctacg cctggctcaa ccaacccgag gcgctgcaag ccttctggca ggcgcagacg 361 cccgcggtac gccagttgct cgaaggctac gccgccggtt tcaaccgctt cctccgcgag 421 gccgacggca agaccaccag ttgccttggc cagccctggc tgcgggccat cgcgaccgat 481 gacctgctgc gcctgacccg gcgcctgctg gtcgaaggcg gggtcggcca gttcgccgac 541 gcgctggtgg ccgccgcgcc gcccggagcg gagaaggtcg ccttgagcgg cgagcaggcg 601 ttccaggtcg ccgagcagcg gcgccagcgc ttccgcctgg agcgcggcag caacgccatt 661 gccgttggca gcgaacgttc ggcggacggc aagggcatgc tcctggccaa cccgcacttc 721 ccctggaacg gcgcgatgcg tttctaccag atgcacctga ccattcccgg ccggctcgac 781 gtgatggggg cctcgctgcc cggcctgccg gtggtcaaca tcggcttcag ccgccacctg 841 gcctggaccc acacggtgga tacctccagc cacttcaccc tgtatcgcct ggcgctggac 901 ccgaaggacc cgcggcgcta cctggtcgac ggtcgttcgc tgccgctgga ggagaagtcc 961 gtcgcgatcg aggtgcgcgg cgccgacggc aagctgtcgc gcgtcgagca caaggtctac 1021 cagtcgatct acggcccgct ggtggtctgg cccggcaagc tggactggaa ccgcagcgag 1081 gcctatgcgc tgcgtgacgc caacctggag aacacccggg tactgcaaca gtggtactcg 1141 atcaaccagg ccagcgacgt cgccgacctg cgccggcgcg tcgaggcgct acaggggatc 1201 ccctgggtca acaccctggc cgcggacgag cagggcaacg ccctgtacat gaaccagtcg 1261 gtggtgccct acctgaagcc ggaactgatt cccgcctgcg ccattccgca actggtcgcc 1321 gaaggcctgc cggccctcca ggggcaggac agccgctgtg cctggagtcg cgacccggcc 1381 gcggcccagg ctggcatcac cccggcggcg caactgccgg tgctgttgcg cagggacttc 1441 gtgcagaact ccaacgacag cgcctggctg accaacccgg cgagcccgct gcaaggcttc 1501 tcgcccctgg tcagccagga gaagcccatc ggtccgcggg cgcgctacgc cctgagccgg 1561 ctacagggca agcagccgct ggaggcgaag acgctcgagg agatggtcac cgccaaccat 1621 gtcttcagcg ccgaccaggt gctgccggac ctgctccgcc tgtgccgcga caaccagggc 1681 gagaagtccc ttgcccgcgc ctgcgcggcc ctggcgcagt gggaccgtgg cgccaacctc 1741 gacagcggca gcggcttcgt ctacttccag cgcttcatgc aacgcttcgc cgaactcgac 1801 ggcgcgtgga aggaaccgtt cgatgcgcaa cgtcccctgg atacgccgca aggcatcgcc 1861 ctcgaccggc cgcaggtggc gacccaggtg cgccaggcgc tggcggacgc ggcggcggag 1921 gtggagaaga gcgggattcc cgacggcgcg cgctggggcg acctgcaagt gagcacccgt 1981 ggccaggaac gcatcgcgat tcccggcggc gatggccatt tcggggtcta caacgcgatc 2041 cagagcgtcc gcaagggcga ccacctggag gtggtcggcg gcactagcta catccagctg 2101 gtgaccttcc ccgaggaagg gcccaaggct cgcgggttgc tggctttctc ccagtccagc 2161 gatccgcgct cgccgcacta ccgcgaccag accgagctgt tttcccgcca gcaatggcag 2221 accttgccgt tcagcgacag gcagatcgac gccgacccgc aactgcaacg gctaagcatt 2281 cgcgaacatc atcaccatca ccactga //