BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation1765 1 A range1765 1 492 492 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation1762 1 prefix range1762 1 1 2 BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2046 1 -35 range2046 1 20 25 annotation2045 1 LuxR/HSL range2045 1 1 20 annotation2048 1 start range2048 1 53 53 annotation2047 1 -10 range2047 1 42 47 annotation7070 1 BBa_R0062 range7070 1 1 55 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K855007 1 BBa_K855007 LuxR generator regulated by Lac Promoter followed plux promoter (R0062) and RBS (B0034) 2012-10-01T11:00:00Z 2015-05-08T01:13:34Z It is extracted from BBa_K137076. It is a ready to use AHL regulated system. After inserting a coding sequence after the RBS (B0034), the protein will be produced in response to detecting acylhomoserine lactones. false false _1115_ 0 13531 9 It's complicated false N/A false Ma Tsz Shan component2269339 1 BBa_B0010 component2269346 1 BBa_R0062 component2269332 1 BBa_B0034 component2269341 1 BBa_B0012 component2269351 1 BBa_B0034 component2269326 1 BBa_R0011 component2269335 1 BBa_C0062 annotation2269326 1 BBa_R0011 range2269326 1 1 54 annotation2269332 1 BBa_B0034 range2269332 1 64 75 annotation2269335 1 BBa_C0062 range2269335 1 82 837 annotation2269346 1 BBa_R0062 range2269346 1 1008 1062 annotation2269339 1 BBa_B0010 range2269339 1 871 950 annotation2269351 1 BBa_B0034 range2269351 1 1071 1082 annotation2269341 1 BBa_B0012 range2269341 1 959 999 BBa_K855005 1 BBa_K855005 pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site 2012-10-01T11:00:00Z 2015-05-08T01:13:34Z pseudomonas aeruginosa PAO1 pvdQ gene codes for an acylase that can degrade long-chain N-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL. A site-directed mutagenesis at 1491 bp is introduced to the leucine codon, CTG > CTC, to remove the internal illegal PstI site. After the mutagenesis, the biobrick will be compatible with the standard assembly and the pvdQ gene expressed will still be functional. false false _1115_ 0 13531 9 It's complicated false A his tag is added at the C-terminal of the gene sequence to help the detection of the expressed protein. false Ma Tsz Shan annotation2207619 1 his tag range2207619 1 2287 2304 annotation2207618 1 stop range2207618 1 2305 2307 annotation2207600 1 Signal peptide range2207600 1 1 69 annotation2207613 1 pvdQ gene range2207613 1 70 2286 annotation2207616 1 silent mutation "G > C" to remove PstI site range2207616 1 1491 1491 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2000 1 -35 range2000 1 20 25 annotation1999 1 lac O1 range1999 1 3 19 annotation2002 1 -10 range2002 1 43 48 annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 BBa_K855008 1 BBa_K855008 AHL-inducible Production of pvdQ (K855005) 2012-10-04T11:00:00Z 2015-05-08T01:13:34Z pseudomonas aeruginosa PAO1 This part requires the external presence acyl homoserine lactones to stimulate the production of pvdQ, but pvdQ produced will degrade the AHL molecules, it is a dynamic system that maintains the AHL concentration at certain level. false false _1115_ 0 13531 9 It's complicated false Internal PstI site of pvdQ is removed by site-directed mutagenesis. false Ma Tsz Shan component2290903 1 BBa_B0010 component2290905 1 BBa_B0012 component2290896 1 BBa_K855007 component2290902 1 BBa_K855005 annotation2290896 1 BBa_K855007 range2290896 1 1 1082 annotation2290905 1 BBa_B0012 range2290905 1 3492 3532 annotation2290902 1 BBa_K855005 range2290902 1 1089 3395 annotation2290903 1 BBa_B0010 range2290903 1 3404 3483 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K855007_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaa BBa_K855005_sequence 1 atggggatgcgtaccgtactgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccgcggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggctacgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggcagcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgctgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcctccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgcctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcggagaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcagcaacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaacggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgccggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcgcctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcgatcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtggtctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtactgcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccctgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccggaactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgcctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttcgtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctccagggcttctcgcccctggtcagccaggagaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcgaggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccagggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggcttcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcccctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcggcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgcatcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggtggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcccagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgttcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactga BBa_K855008_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggggatgcgtaccgtactgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccgcggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggctacgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggcagcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgctgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcctccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgcctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcggagaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcagcaacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaacggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgccggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcgcctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcgatcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtggtctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtactgcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccctgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccggaactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgcctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttcgtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctccagggcttctcgcccctggtcagccaggagaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcgaggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccagggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggcttcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcccctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcggcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgcatcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggtggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcccagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgttcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z