LOCUS BBa_K855009 3532 bp DNA linear 25-Nov-2024 DEFINITION AHL-inducible Production of pvdQ (K855006) ACCESSION BBa_K855009 VERSION BBa_K855009.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1..1082 /label=BBa_K855007 misc_feature 1089..1157 /label=signal peptide CDS 1158..3374 /label=pvdQ gene misc_feature 2582..2582 /label=silent mutation "G > A" to remove the PstI site misc_feature 3393..3395 /label=stop misc_feature 3375..3392 /label=his tag CDS 1089..3395 /label=BBa_K855006 stem_loop 3415..3458 /label=stem_loop misc_feature 3404..3483 /label=BBa_B0010 terminator 3404..3483 /label=BBa_B0010 stem_loop 3499..3518 /label=T7 TE misc_feature 3525..3525 /label=stop polyA_site 3519..3532 /label=polya misc_feature 3492..3532 /label=BBa_B0012 terminator 3492..3532 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat ggggatgcgt accgtactga ccggcctggc cggcatgctg ttgggttcga 1141 tgatgccggt ccaggccgat atgccgcggc cgaccgggct ggccgcggat atccgctgga 1201 ccgcctatgg cgtgccgcac atccgggcca aggatgagcg cggcctgggc tatggcatcg 1261 gctacgccta cgcgcgcgac aacgcctgcc tgctggccga ggagatcgtc accgcgcgcg 1321 gcgagcgggc gcgctatttc ggcagcgagg gcaagtcgtc ggccgagctg gacaacctgc 1381 cgtccgacat cttctacgcc tggctcaacc aacccgaggc gctgcaagcc ttctggcagg 1441 cgcagacgcc cgcggtacgc cagttgctcg aaggctacgc cgccggtttc aaccgcttcc 1501 tccgcgaggc cgacggcaag accaccagtt gccttggcca gccctggctg cgggccatcg 1561 cgaccgatga cctgctgcgc ctgacccggc gcctgctggt cgaaggcggg gtcggccagt 1621 tcgccgacgc gctggtggcc gccgcgccgc ccggagcgga gaaggtcgcc ttgagcggcg 1681 agcaggcgtt ccaggtcgcc gagcagcggc gccagcgctt ccgcctggag cgcggcagca 1741 acgccattgc cgttggcagc gaacgttcgg cggacggcaa gggcatgctc ctggccaacc 1801 cgcacttccc ctggaacggc gcgatgcgtt tctaccagat gcacctgacc attcccggcc 1861 ggctcgacgt gatgggggcc tcgctgcccg gcctgccggt ggtcaacatc ggcttcagcc 1921 gccacctggc ctggacccac acggtggata cctccagcca cttcaccctg tatcgcctgg 1981 cgctggaccc gaaggacccg cggcgctacc tggtcgacgg tcgttcgctg ccgctggagg 2041 agaagtccgt cgcgatcgag gtgcgcggcg ccgacggcaa gctgtcgcgc gtcgagcaca 2101 aggtctacca gtcgatctac ggcccgctgg tggtctggcc cggcaagctg gactggaacc 2161 gcagcgaggc ctatgcgctg cgtgacgcca acctggagaa cacccgggta ctgcaacagt 2221 ggtactcgat caaccaggcc agcgacgtcg ccgacctgcg ccggcgcgtc gaggcgctac 2281 aggggatccc ctgggtcaac accctggccg cggacgagca gggcaacgcc ctgtacatga 2341 accagtcggt ggtgccctac ctgaagccgg aactgattcc cgcctgcgcc attccgcaac 2401 tggtcgccga aggcctgccg gccctccagg ggcaggacag ccgctgtgcc tggagtcgcg 2461 acccggccgc ggcccaggct ggcatcaccc cggcggcgca actgccggtg ctgttgcgca 2521 gggacttcgt gcagaactcc aacgacagcg cctggctgac caacccggcg agcccgctgc 2581 aaggcttctc gcccctggtc agccaggaga agcccatcgg tccgcgggcg cgctacgccc 2641 tgagccggct acagggcaag cagccgctgg aggcgaagac gctcgaggag atggtcaccg 2701 ccaaccatgt cttcagcgcc gaccaggtgc tgccggacct gctccgcctg tgccgcgaca 2761 accagggcga gaagtccctt gcccgcgcct gcgcggccct ggcgcagtgg gaccgtggcg 2821 ccaacctcga cagcggcagc ggcttcgtct acttccagcg cttcatgcaa cgcttcgccg 2881 aactcgacgg cgcgtggaag gaaccgttcg atgcgcaacg tcccctggat acgccgcaag 2941 gcatcgccct cgaccggccg caggtggcga cccaggtgcg ccaggcgctg gcggacgcgg 3001 cggcggaggt ggagaagagc gggattcccg acggcgcgcg ctggggcgac ctgcaagtga 3061 gcacccgtgg ccaggaacgc atcgcgattc ccggcggcga tggccatttc ggggtctaca 3121 acgcgatcca gagcgtccgc aagggcgacc acctggaggt ggtcggcggc actagctaca 3181 tccagctggt gaccttcccc gaggaagggc ccaaggctcg cgggttgctg gctttctccc 3241 agtccagcga tccgcgctcg ccgcactacc gcgaccagac cgagctgttt tcccgccagc 3301 aatggcagac cttgccgttc agcgacaggc agatcgacgc cgacccgcaa ctgcaacggc 3361 taagcattcg cgaacatcat caccatcacc actgatacta gagccaggca tcaaataaaa 3421 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3481 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //