##gff-version 3
##sequence-region BBa_K855009 1 3532
BBa_K855009	.	engineered_region	1	1082	.	+	0	ID=BBa_K855007;Name=BBa_K855007
BBa_K855009	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K855007
BBa_K855009	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K855009	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K855009	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K855009	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K855009	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K855009	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K855007
BBa_K855009	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K855009	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K855009	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K855009	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K855009	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K855007
BBa_K855009	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K855009	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K855009	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K855009	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K855009	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K855009	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K855009	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K855007
BBa_K855009	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K855009	.	ribosome_entry_site	1071	1082	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K855007
BBa_K855009	.	conserved_region	1075	1078	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K855009	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K855007
BBa_K855009	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K855009	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K855009	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K855007
BBa_K855009	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K855009	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K855009	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K855009	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K855009	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K855009	.	terminator	3404	3483	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K855009	.	engineered_region	3404	3483	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K855009	.	stem_loop	3415	3458	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K855009	.	CDS	1089	3395	.	+	0	ID=BBa_K855006;Name=BBa_K855006
BBa_K855009	.	tag	3375	3392	.	+	0	ID=annotation2207647;Name=his tag;Parent=BBa_K855006
BBa_K855009	.	CDS	1158	3374	.	+	0	ID=annotation2207631;Name=pvdQ gene;Parent=BBa_K855006
BBa_K855009	.	stop_codon	3393	3395	.	+	0	ID=annotation2207638;Name=stop;Parent=BBa_K855006
BBa_K855009	.	sequence_alteration	2582	2582	.	+	0	ID=annotation2207634;Name=silent mutation "G > A" to remove the PstI site;Parent=BBa_K855006
BBa_K855009	.	sequence_feature	1089	1157	.	+	0	ID=annotation2207627;Name=signal peptide;Parent=BBa_K855006
BBa_K855009	.	terminator	3492	3532	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K855009	.	stop_codon	3525	3525	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K855009	.	polyA_site	3519	3532	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K855009	.	stem_loop	3499	3518	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K855009	.	engineered_region	3492	3532	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K855009
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggggatgcgtaccgta
ctgaccggcctggccggcatgctgttgggttcgatgatgccggtccaggccgatatgccgcggccgaccgggctggccg
cggatatccgctggaccgcctatggcgtgccgcacatccgggccaaggatgagcgcggcctgggctatggcatcggcta
cgcctacgcgcgcgacaacgcctgcctgctggccgaggagatcgtcaccgcgcgcggcgagcgggcgcgctatttcggc
agcgagggcaagtcgtcggccgagctggacaacctgccgtccgacatcttctacgcctggctcaaccaacccgaggcgc
tgcaagccttctggcaggcgcagacgcccgcggtacgccagttgctcgaaggctacgccgccggtttcaaccgcttcct
ccgcgaggccgacggcaagaccaccagttgccttggccagccctggctgcgggccatcgcgaccgatgacctgctgcgc
ctgacccggcgcctgctggtcgaaggcggggtcggccagttcgccgacgcgctggtggccgccgcgccgcccggagcgg
agaaggtcgccttgagcggcgagcaggcgttccaggtcgccgagcagcggcgccagcgcttccgcctggagcgcggcag
caacgccattgccgttggcagcgaacgttcggcggacggcaagggcatgctcctggccaacccgcacttcccctggaac
ggcgcgatgcgtttctaccagatgcacctgaccattcccggccggctcgacgtgatgggggcctcgctgcccggcctgc
cggtggtcaacatcggcttcagccgccacctggcctggacccacacggtggatacctccagccacttcaccctgtatcg
cctggcgctggacccgaaggacccgcggcgctacctggtcgacggtcgttcgctgccgctggaggagaagtccgtcgcg
atcgaggtgcgcggcgccgacggcaagctgtcgcgcgtcgagcacaaggtctaccagtcgatctacggcccgctggtgg
tctggcccggcaagctggactggaaccgcagcgaggcctatgcgctgcgtgacgccaacctggagaacacccgggtact
gcaacagtggtactcgatcaaccaggccagcgacgtcgccgacctgcgccggcgcgtcgaggcgctacaggggatcccc
tgggtcaacaccctggccgcggacgagcagggcaacgccctgtacatgaaccagtcggtggtgccctacctgaagccgg
aactgattcccgcctgcgccattccgcaactggtcgccgaaggcctgccggccctccaggggcaggacagccgctgtgc
ctggagtcgcgacccggccgcggcccaggctggcatcaccccggcggcgcaactgccggtgctgttgcgcagggacttc
gtgcagaactccaacgacagcgcctggctgaccaacccggcgagcccgctgcaaggcttctcgcccctggtcagccagg
agaagcccatcggtccgcgggcgcgctacgccctgagccggctacagggcaagcagccgctggaggcgaagacgctcga
ggagatggtcaccgccaaccatgtcttcagcgccgaccaggtgctgccggacctgctccgcctgtgccgcgacaaccag
ggcgagaagtcccttgcccgcgcctgcgcggccctggcgcagtgggaccgtggcgccaacctcgacagcggcagcggct
tcgtctacttccagcgcttcatgcaacgcttcgccgaactcgacggcgcgtggaaggaaccgttcgatgcgcaacgtcc
cctggatacgccgcaaggcatcgccctcgaccggccgcaggtggcgacccaggtgcgccaggcgctggcggacgcggcg
gcggaggtggagaagagcgggattcccgacggcgcgcgctggggcgacctgcaagtgagcacccgtggccaggaacgca
tcgcgattcccggcggcgatggccatttcggggtctacaacgcgatccagagcgtccgcaagggcgaccacctggaggt
ggtcggcggcactagctacatccagctggtgaccttccccgaggaagggcccaaggctcgcgggttgctggctttctcc
cagtccagcgatccgcgctcgccgcactaccgcgaccagaccgagctgttttcccgccagcaatggcagaccttgccgt
tcagcgacaggcagatcgacgccgacccgcaactgcaacggctaagcattcgcgaacatcatcaccatcaccactgata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata