BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_K861021 1 BBa_K861021 FadE gene for fatty acid degradation from <i>Salmonella enterica</i> LT2 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE have far better efficiency compared to FadE in E.coli. The original S-FadE gene contains PstI. This parts has already been point mutated. false false _1121_ 0 12357 9 Not in stock false None false Kuanwei Sheng BBa_K861023 1 BBa_K861023 S-FadE with a RBS and a terminator 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE have far better efficiency compared to FadE in E.coli. The original S-FadE gene contains PstI. This parts has already been point mutated. false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng component2182584 1 BBa_B0024 component2182583 1 BBa_K861021 component2182581 1 BBa_B0030 annotation2182581 1 BBa_B0030 range2182581 1 1 15 annotation2182584 1 BBa_B0024 range2182584 1 2622 2716 annotation2182583 1 BBa_K861021 range2182583 1 22 2613 BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861021_sequence 1 atgatgattttgagtattatcgctacggttgttctgctcggcgcattgttctatcaccgcgtgagtttatttctcagcagcctgattctgctcgcctggaccgccgcacttggcgtcgctggcctgtggtccatctggctgttggttatgatgattttgagtattatcgctacggttgttctgctcggcgcattgttctatcaccgcgtgagtttatttctcagcagcctgattctgctcgcctggaccgccgcacttggcgtcgctggcctgtggtccatctggctgttggttcctctggccattatcctcgtgccgtttaacctcacaccgatgcgaaaatcaatgatttccgcaccggtgttccgcggcttccgtaaggtcatgccgccgatgtcgcgcactgagaaagaagcgattgatgccggtacaacctggtgggaaggcgatctgttccagggcaagcctgactggaaaaaactgcacaactatccgcaaccgcaactcactgccgaagagcaagcgtttctcgacggtccggtagaagaagcgtgccggatggcgaacgatttccagattacccatgaactggcggatctgccgccggaactgtgggcttatctgaaagagcatcgtttttttgcgatgatcattaaaaaagagtacggcggactggagttctccgcttatgcgcagtcccgtgtactgcaaaaactgtccggcgtctccgggattctggctatcaccgtcggcgttccaaactcactcggccctggcgagttgttgcagcactatggtacggaagagcagaagaatcactatctgccgcgactggcgcgcggccaggagatcccctgctttgcgctgaccagcccggaagcaggttctgatgcaggcgccattccggataccggcgtggtttgcatgggcgaatggcagggccagcaggtgctgggtatgcgcctgacctggaacaagcgttacattacgctggcaccgatagctaccgtgttgggcctggcgtttaagctttccgatccggatagattgttaggcggagaagaagagctgggtattacctgtgccctgatccccacctcaacgccgggtgtggaaattggtcgtcgtcacttcccgcttaacgttccgttccagaatggtccaacccgcggtaacgatattttcgtgcctattgattatattatcggcggccccaaaatggccggacaaggctggcgtatgctggtggaatgtttgtcggtaggacgcggtattaccctgccgtccaactccactggcggggtgaaatccgtcgcgctggcaaccggcgcctatgctcacattcgtcgtcagttcaaaatatctatcggcaagatggaaggtattgaggaaccgctggcgcgtattgcaggtaacgcttatgtgatggatgccgccgcatcgttaatcacctatggcattatgctgggtgaaaaaccggcggtactgtcggctatcgtgaagtaccactgcacccaccgtggccagcagtccattatcgacgcgatggatatcactgggggtaaaggcattatgctgggcgagagcaacttcctggcgcgtgcttaccagggcgcgcctatcgcgatcaccgtggaaggcgcgaatatcctgacccgtagcatgatgatctttggtcagggcgccattcgctgccatccttatgtactggaagagatggccgcagcgcagaataatgatgtgaatgcctttgataagctgttgtttaaacatatcggccatgtcggcagcaataccgtccgcagtttctggcttggcctgacccgcggattaacgagccatacgccaaccggcgacgccacgaagcgttattaccagcatctgaaccgactgagcgcaaacctggcgctgctgtctgacgtgtcaatggcggtactgggcggcagcctgaaacgccgcgagcgtatctccgcgcgtctgggcgatgtgttgagtcagctttatctggcctcagccgtactgaagcgttacgacgacgaaggtcgccacgaagccgacctgccgctggtgcactggggcgtacaggatgcgctgtatcgtgctgaacaagcgatggacgatctgctgcaaaacttcccgaaccgcgtcgtggccggattgctcaccgcgatgatcttcccgactggccgtcattatctggcgccgtctgataagctggatcacgccgtggcgaagatcctacaggtgccgaacgccacacgttcacgcattgggcgcggtcagtatctgacgcccgccgaacataatccggtcggtttactggaagaagcgctgcgtgatgtcatcgctgccgaccctatccaccagcgcatctgcaaagaactgggtaaaaatctgccgtttactcgtctggatgaactggcgcgcaatgcgctggcgaaagggcttatagataaagatgaagccgccatcctggcgaaagcggaagagagccgcctgcgcagtatcaacgttgatgattttgaacctgaagcgctggcgaccaaaccggtaaagctgccggagaaagtgcgtaaagtcgaagccgcataa BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861023_sequence 1 attaaagaggagaaatactagatgatgattttgagtattatcgctacggttgttctgctcggcgcattgttctatcaccgcgtgagtttatttctcagcagcctgattctgctcgcctggaccgccgcacttggcgtcgctggcctgtggtccatctggctgttggttatgatgattttgagtattatcgctacggttgttctgctcggcgcattgttctatcaccgcgtgagtttatttctcagcagcctgattctgctcgcctggaccgccgcacttggcgtcgctggcctgtggtccatctggctgttggttcctctggccattatcctcgtgccgtttaacctcacaccgatgcgaaaatcaatgatttccgcaccggtgttccgcggcttccgtaaggtcatgccgccgatgtcgcgcactgagaaagaagcgattgatgccggtacaacctggtgggaaggcgatctgttccagggcaagcctgactggaaaaaactgcacaactatccgcaaccgcaactcactgccgaagagcaagcgtttctcgacggtccggtagaagaagcgtgccggatggcgaacgatttccagattacccatgaactggcggatctgccgccggaactgtgggcttatctgaaagagcatcgtttttttgcgatgatcattaaaaaagagtacggcggactggagttctccgcttatgcgcagtcccgtgtactgcaaaaactgtccggcgtctccgggattctggctatcaccgtcggcgttccaaactcactcggccctggcgagttgttgcagcactatggtacggaagagcagaagaatcactatctgccgcgactggcgcgcggccaggagatcccctgctttgcgctgaccagcccggaagcaggttctgatgcaggcgccattccggataccggcgtggtttgcatgggcgaatggcagggccagcaggtgctgggtatgcgcctgacctggaacaagcgttacattacgctggcaccgatagctaccgtgttgggcctggcgtttaagctttccgatccggatagattgttaggcggagaagaagagctgggtattacctgtgccctgatccccacctcaacgccgggtgtggaaattggtcgtcgtcacttcccgcttaacgttccgttccagaatggtccaacccgcggtaacgatattttcgtgcctattgattatattatcggcggccccaaaatggccggacaaggctggcgtatgctggtggaatgtttgtcggtaggacgcggtattaccctgccgtccaactccactggcggggtgaaatccgtcgcgctggcaaccggcgcctatgctcacattcgtcgtcagttcaaaatatctatcggcaagatggaaggtattgaggaaccgctggcgcgtattgcaggtaacgcttatgtgatggatgccgccgcatcgttaatcacctatggcattatgctgggtgaaaaaccggcggtactgtcggctatcgtgaagtaccactgcacccaccgtggccagcagtccattatcgacgcgatggatatcactgggggtaaaggcattatgctgggcgagagcaacttcctggcgcgtgcttaccagggcgcgcctatcgcgatcaccgtggaaggcgcgaatatcctgacccgtagcatgatgatctttggtcagggcgccattcgctgccatccttatgtactggaagagatggccgcagcgcagaataatgatgtgaatgcctttgataagctgttgtttaaacatatcggccatgtcggcagcaataccgtccgcagtttctggcttggcctgacccgcggattaacgagccatacgccaaccggcgacgccacgaagcgttattaccagcatctgaaccgactgagcgcaaacctggcgctgctgtctgacgtgtcaatggcggtactgggcggcagcctgaaacgccgcgagcgtatctccgcgcgtctgggcgatgtgttgagtcagctttatctggcctcagccgtactgaagcgttacgacgacgaaggtcgccacgaagccgacctgccgctggtgcactggggcgtacaggatgcgctgtatcgtgctgaacaagcgatggacgatctgctgcaaaacttcccgaaccgcgtcgtggccggattgctcaccgcgatgatcttcccgactggccgtcattatctggcgccgtctgataagctggatcacgccgtggcgaagatcctacaggtgccgaacgccacacgttcacgcattgggcgcggtcagtatctgacgcccgccgaacataatccggtcggtttactggaagaagcgctgcgtgatgtcatcgctgccgaccctatccaccagcgcatctgcaaagaactgggtaaaaatctgccgtttactcgtctggatgaactggcgcgcaatgcgctggcgaaagggcttatagataaagatgaagccgccatcctggcgaaagcggaagagagccgcctgcgcagtatcaacgttgatgattttgaacctgaagcgctggcgaccaaaccggtaaagctgccggagaaagtgcgtaaagtcgaagccgcataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z