BBa_K861038
type: ComponentDefinition
persistent identity: BBa K861038
display id: BBa_K861038
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG induced S-FadA and S-FadB
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_R0011
component: BBa_K861035
component: BBa_K861045
sequence annotation: BBa_R0011
sequence annotation: BBa_K861045
sequence annotation: BBa_K861035
sequence: https://synbiohub.org/public/igem/BBa_K861038_sequence/1

BBa_R0011
type: Component
persistent identity: component2190681
display id: component2190681
version: 1
top level: BBa_K861038
definition: lacI+pL
access: public

BBa_K861035
type: Component
persistent identity: component2190697
display id: component2190697
version: 1
top level: BBa_K861038
definition: BBa_K861035
access: public

BBa_K861045
type: Component
persistent identity: component2190691
display id: component2190691
version: 1
top level: BBa_K861038
definition: BBa_K861045
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2190681
display id: annotation2190681
version: 1
top level: BBa_K861038
location: https://synbiohub.org/public/igem/BBa_K861038/annotation2190681/range2190681/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K861038/annotation2190681/range2190681/1
type: Range
persistent identity: range2190681
display id: range2190681
version: 1
top level: BBa_K861038
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K861045
type: SequenceAnnotation
persistent identity: annotation2190691
display id: annotation2190691
version: 1
top level: BBa_K861038
location: https://synbiohub.org/public/igem/BBa_K861038/annotation2190691/range2190691/1
component: BBa_K861045

https://synbiohub.org/public/igem/BBa_K861038/annotation2190691/range2190691/1
type: Range
persistent identity: range2190691
display id: range2190691
version: 1
top level: BBa_K861038
direction: direction/reverse
start: 64
end: 1351
orientation: inline

BBa_K861035
type: SequenceAnnotation
persistent identity: annotation2190697
display id: annotation2190697
version: 1
top level: BBa_K861038
location: https://synbiohub.org/public/igem/BBa_K861038/annotation2190697/range2190697/1
component: BBa_K861035

https://synbiohub.org/public/igem/BBa_K861038/annotation2190697/range2190697/1
type: Range
persistent identity: range2190697
display id: range2190697
version: 1
top level: BBa_K861038
direction: direction/reverse
start: 1360
end: 3673
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K861035
type: ComponentDefinition
persistent identity: BBa K861035
display id: BBa_K861035
version: 1
was generated by: iGEM to SBOL conversion
description: S-FadB with a RBS and a terminator
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
mutable description: S-FadB gene from Salmonella enterica LT2 functions as FadB gene in E.coli.This device contains S-FadB with a RBS and a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0030
component: BBa_B0024
component: BBa_K861032
sequence annotation: BBa_B0030
sequence annotation: BBa_K861032
sequence annotation: BBa_B0024
sequence: https://synbiohub.org/public/igem/BBa_K861035_sequence/1

BBa_B0030
type: Component
persistent identity: component2181866
display id: component2181866
version: 1
top level: BBa_K861035
definition: BBa_B0030
access: public

BBa_B0024
type: Component
persistent identity: component2181869
display id: component2181869
version: 1
top level: BBa_K861035
definition: BBa_B0024
access: public

BBa_K861032
type: Component
persistent identity: component2181868
display id: component2181868
version: 1
top level: BBa_K861035
definition: BBa_K861032
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2181866
display id: annotation2181866
version: 1
top level: BBa_K861035
location: https://synbiohub.org/public/igem/BBa_K861035/annotation2181866/range2181866/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K861035/annotation2181866/range2181866/1
type: Range
persistent identity: range2181866
display id: range2181866
version: 1
top level: BBa_K861035
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K861032
type: SequenceAnnotation
persistent identity: annotation2181868
display id: annotation2181868
version: 1
top level: BBa_K861035
location: https://synbiohub.org/public/igem/BBa_K861035/annotation2181868/range2181868/1
component: BBa_K861032

https://synbiohub.org/public/igem/BBa_K861035/annotation2181868/range2181868/1
type: Range
persistent identity: range2181868
display id: range2181868
version: 1
top level: BBa_K861035
direction: direction/reverse
start: 22
end: 2211
orientation: inline

BBa_B0024
type: SequenceAnnotation
persistent identity: annotation2181869
display id: annotation2181869
version: 1
top level: BBa_K861035
location: https://synbiohub.org/public/igem/BBa_K861035/annotation2181869/range2181869/1
component: BBa_B0024

https://synbiohub.org/public/igem/BBa_K861035/annotation2181869/range2181869/1
type: Range
persistent identity: range2181869
display id: range2181869
version: 1
top level: BBa_K861035
direction: direction/reverse
start: 2220
end: 2314
orientation: inline

BBa_K861045
type: ComponentDefinition
persistent identity: BBa K861045
display id: BBa_K861045
version: 1
was generated by: iGEM to SBOL conversion
description: S-FadA with a RBS and a terminator
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
mutable description: In the process of LCFA degradation,the cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.S-FadA from S. enterica functions as FadA in E.coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K861042
component: BBa_B0030
component: BBa_B0024
sequence annotation: BBa_B0030
sequence annotation: BBa_K861042
sequence annotation: BBa_B0024
sequence: https://synbiohub.org/public/igem/BBa_K861045_sequence/1

BBa_K861042
type: Component
persistent identity: component2181890
display id: component2181890
version: 1
top level: BBa_K861045
definition: BBa_K861042
access: public

BBa_B0030
type: Component
persistent identity: component2181888
display id: component2181888
version: 1
top level: BBa_K861045
definition: BBa_B0030
access: public

BBa_B0024
type: Component
persistent identity: component2181891
display id: component2181891
version: 1
top level: BBa_K861045
definition: BBa_B0024
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2181888
display id: annotation2181888
version: 1
top level: BBa_K861045
location: https://synbiohub.org/public/igem/BBa_K861045/annotation2181888/range2181888/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K861045/annotation2181888/range2181888/1
type: Range
persistent identity: range2181888
display id: range2181888
version: 1
top level: BBa_K861045
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K861042
type: SequenceAnnotation
persistent identity: annotation2181890
display id: annotation2181890
version: 1
top level: BBa_K861045
location: https://synbiohub.org/public/igem/BBa_K861045/annotation2181890/range2181890/1
component: BBa_K861042

https://synbiohub.org/public/igem/BBa_K861045/annotation2181890/range2181890/1
type: Range
persistent identity: range2181890
display id: range2181890
version: 1
top level: BBa_K861045
direction: direction/reverse
start: 22
end: 1185
orientation: inline

BBa_B0024
type: SequenceAnnotation
persistent identity: annotation2181891
display id: annotation2181891
version: 1
top level: BBa_K861045
location: https://synbiohub.org/public/igem/BBa_K861045/annotation2181891/range2181891/1
component: BBa_B0024

https://synbiohub.org/public/igem/BBa_K861045/annotation2181891/range2181891/1
type: Range
persistent identity: range2181891
display id: range2181891
version: 1
top level: BBa_K861045
direction: direction/reverse
start: 1194
end: 1288
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_K861032
type: ComponentDefinition
persistent identity: BBa K861032
display id: BBa_K861032
version: 1
was generated by: iGEM to SBOL conversion
description: S-FadB gene for fatty acid degradation from Salmonella enterica LT2
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
mutable description: FadB gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadB have far better efficiency compared to FadB in E.coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K861032_sequence/1

BBa_K861042
type: ComponentDefinition
persistent identity: BBa K861042
display id: BBa_K861042
version: 1
was generated by: iGEM to SBOL conversion
description: FadA, gene for fatty acid degradation from Salmonella enterica LT2
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FadA gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.(???S-??? is used to distinguish FadA gene in E.coli )
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K861042_sequence/1

https://synbiohub.org/public/igem/BBa_K861038_sequence/1
type: Sequence
persistent identity: BBa K861038 sequence
display id: BBa_K861038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatggaacaggttgtcattgtcgatgctattcgcaccccgatgggccgttcgaagggcggcgcgtttcgcaacgtgcgagcagaagatctctccgcccacttaatgcgtagcctgctggcgcgtaatccgtcgcttacagcggcgaccctcgatgatatttactggggctgcgtacaacaaacgctggagcaaggcttcaacattgcccgtaacgccgcgctgctggcagaaattccccattcggtaccggcggtcaccgtcaaccgtctgtgtggttcctcgatgcaggcgttacacgatgcagcgcgaatgatcatgaccggcgatgcgcaggtttgtctggttggcggcgtggagcatatggggcacgtgccgatgagccacggcgtggattttcacccgggtctgagtcgcaacgtcgccaaagcggcagggatgatggggctaacggcggaaatgctctcccgcctgcacggcattagccgggaaatgcaggaccagttcgccgcgcgttctcacgctcgcgcctgggccgccacccagtctggcgcattcaaaacggagattatcccgactggcggtcatgatgcagacggcgtgttgaagcagtttaactacgatgaagtgatccgcccggaaaccacggtcgaagcgctatcaacgctgcgtccggcatttgatccggttagtggcacggtcacggcgggcacctcatccgcgctttccgatggcgcagccgccatgctggtaatgagcgaaagtcgtgcccgtgagctgggtctgaaacctcgcgcccgtattcgctcaatggcagtggtgggttgtgatccgtcaattatgggttacgggccggttccggcgtcaaaactggcgttgaaaaaagcgggactgtcagccagcgatatcgatgtgtttgagatgaacgaagcgtttgccgcacagatcctgccatgcattaaggatctgggattgatggagcagatagacgagaagatcaacctcaacggcggtgcgatcgcgcttggtcatccgctcggctgctccggagcacgtatcagcaccacgcttatcaacctgatggagcgcaaagacgcgcagtttggtctggcgacgatgtgtattggtctgggtcagggcatcgccacggtgtttgagcgggtttaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgatactagagattaaagaggagaaatactagatgctttataaaggcgacaccctgtacctcgactggctggaagatggcatcgccgaactggtgttcgatgctccaggctcggtcaataaactcgacaccgcaaccgtcgccagcctcggccaggcgcttgaggtgctggaaaagcaacacgatttaaaagggctgctgctgcgctctaataaagcggcctttattgtcggcgcggacatcaccgaatttctttcactgttcctcgtccccgaagagcagttaagccaatggctgcatttcgctaatagcgtctttaaccgtctggaagatttacccgtaccgacccttgccgccgtaaacggctatgcgctgggcggcgggtgcgaatgcgtgctggccaccgattaccgtctggcgacgccagacctccgtatcggtctgccggaaactaaactgggcatcatgccgggtttcggcggctccgttcgtctgccgcgtatgctgggcgcagacagcgcgctggagattatcgcggcaggtaaagatgtcggtgctgagcacgcgctgaaaatcggccttgtggacggcgtggtcaaacaagagaagctaattgaaggcgcgatagcggtgttacgccaggccattaccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861035_sequence/1
type: Sequence
persistent identity: BBa K861035 sequence
display id: BBa_K861035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgctttataaaggcgacaccctgtacctcgactggctggaagatggcatcgccgaactggtgttcgatgctccaggctcggtcaataaactcgacaccgcaaccgtcgccagcctcggccaggcgcttgaggtgctggaaaagcaacacgatttaaaagggctgctgctgcgctctaataaagcggcctttattgtcggcgcggacatcaccgaatttctttcactgttcctcgtccccgaagagcagttaagccaatggctgcatttcgctaatagcgtctttaaccgtctggaagatttacccgtaccgacccttgccgccgtaaacggctatgcgctgggcggcgggtgcgaatgcgtgctggccaccgattaccgtctggcgacgccagacctccgtatcggtctgccggaaactaaactgggcatcatgccgggtttcggcggctccgttcgtctgccgcgtatgctgggcgcagacagcgcgctggagattatcgcggcaggtaaagatgtcggtgctgagcacgcgctgaaaatcggccttgtggacggcgtggtcaaacaagagaagctaattgaaggcgcgatagcggtgttacgccaggccattaccggcgatctcgactggagagctaaacgccagccgaaactggaaccgttgaaactcagtaagattgaagcagcgatgagctttaccatcgctaaaggcatggtcgcacaaacggcgggtaaacattatccggcgccgatgaccgcagtgaaaaccattgaagccgccgcgcgttttggtcgtgaagaagcgctgaatctggaaaataaaagctttgttccgctggcgcataccaatgaggctcgtgcgctggtcggtatcttcctcaacgatcaatatgtaaaaggtaaagccaaaaagctgaccaaagatattgagacgccgaaacaggcagccgtactgggcgcgggcattatgggcggcggtatcgcttaccagtcggcctggaaaggcgtaccggtcatcatgaaagatatcaacgataaatcgttgaacctcggcatgaccgaagcggccaagctgctgaacaaacagctggagcgcggcaagatcgacggtctgaagctggcgggcgtcatttcgaccatccacccgactctcgattatgccggtttcgatcgcgtagatgttgttgtcgaagcggtagtcgaaaatccgaaagtcaaaaaagcggtactggcggaaaccgagcagaaagtgcgcccggaaaccgtgctggcctcaaatacctccaccatcccaattggcgaactggcaagcgccctggaacgtccggaaaacttttgcggaatgcacttcttcaacccggtacaccggatgccgctggttgaaatcattcgcggcgagaagagttcagacgaaaccattgccaaagtcgtcgcctgggccagcaaaatgggcaagacaccgattgtagtcaacgactgccccggcttctttgttaaccgcgtgctgttcccctatttcgccggtttcagtcaactgctgcgcgacggcgcggatttccgcaaagtcgataaagtgatggaaaaacagttcggctggccgatgggcccggcctatctgctcgacgtggtgggtattgataccgcccaccacgcgcaggcagtaatggcggcaggcttcccacagcgtatgcagaaagattaccgcgacgcgatcgacgcattgtttgacgccagccgttttgggcaaaaaaacggtctgggcttctggcgctataaagaagacagcaagggtaagccgaagaaagaagaagatgccgcggtggatgacctgctggcaagcgtcagccagccgaaacgcgacttcagcgacgacgagataatcgcccgtatgatgatcccgatgattaacgaagtggttcgctgtctggaagaaggcattatcgcaagcccggcggaagccgatatggcgctggtcta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861045_sequence/1
type: Sequence
persistent identity: BBa K861045 sequence
display id: BBa_K861045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggaacaggttgtcattgtcgatgctattcgcaccccgatgggccgttcgaagggcggcgcgtttcgcaacgtgcgagcagaagatctctccgcccacttaatgcgtagcctgctggcgcgtaatccgtcgcttacagcggcgaccctcgatgatatttactggggctgcgtacaacaaacgctggagcaaggcttcaacattgcccgtaacgccgcgctgctggcagaaattccccattcggtaccggcggtcaccgtcaaccgtctgtgtggttcctcgatgcaggcgttacacgatgcagcgcgaatgatcatgaccggcgatgcgcaggtttgtctggttggcggcgtggagcatatggggcacgtgccgatgagccacggcgtggattttcacccgggtctgagtcgcaacgtcgccaaagcggcagggatgatggggctaacggcggaaatgctctcccgcctgcacggcattagccgggaaatgcaggaccagttcgccgcgcgttctcacgctcgcgcctgggccgccacccagtctggcgcattcaaaacggagattatcccgactggcggtcatgatgcagacggcgtgttgaagcagtttaactacgatgaagtgatccgcccggaaaccacggtcgaagcgctatcaacgctgcgtccggcatttgatccggttagtggcacggtcacggcgggcacctcatccgcgctttccgatggcgcagccgccatgctggtaatgagcgaaagtcgtgcccgtgagctgggtctgaaacctcgcgcccgtattcgctcaatggcagtggtgggttgtgatccgtcaattatgggttacgggccggttccggcgtcaaaactggcgttgaaaaaagcgggactgtcagccagcgatatcgatgtgtttgagatgaacgaagcgtttgccgcacagatcctgccatgcattaaggatctgggattgatggagcagatagacgagaagatcaacctcaacggcggtgcgatcgcgcttggtcatccgctcggctgctccggagcacgtatcagcaccacgcttatcaacctgatggagcgcaaagacgcgcagtttggtctggcgacgatgtgtattggtctgggtcagggcatcgccacggtgtttgagcgggtttaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0024_sequence/1
type: Sequence
persistent identity: BBa B0024 sequence
display id: BBa_B0024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861032_sequence/1
type: Sequence
persistent identity: BBa K861032 sequence
display id: BBa_K861032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctttataaaggcgacaccctgtacctcgactggctggaagatggcatcgccgaactggtgttcgatgctccaggctcggtcaataaactcgacaccgcaaccgtcgccagcctcggccaggcgcttgaggtgctggaaaagcaacacgatttaaaagggctgctgctgcgctctaataaagcggcctttattgtcggcgcggacatcaccgaatttctttcactgttcctcgtccccgaagagcagttaagccaatggctgcatttcgctaatagcgtctttaaccgtctggaagatttacccgtaccgacccttgccgccgtaaacggctatgcgctgggcggcgggtgcgaatgcgtgctggccaccgattaccgtctggcgacgccagacctccgtatcggtctgccggaaactaaactgggcatcatgccgggtttcggcggctccgttcgtctgccgcgtatgctgggcgcagacagcgcgctggagattatcgcggcaggtaaagatgtcggtgctgagcacgcgctgaaaatcggccttgtggacggcgtggtcaaacaagagaagctaattgaaggcgcgatagcggtgttacgccaggccattaccggcgatctcgactggagagctaaacgccagccgaaactggaaccgttgaaactcagtaagattgaagcagcgatgagctttaccatcgctaaaggcatggtcgcacaaacggcgggtaaacattatccggcgccgatgaccgcagtgaaaaccattgaagccgccgcgcgttttggtcgtgaagaagcgctgaatctggaaaataaaagctttgttccgctggcgcataccaatgaggctcgtgcgctggtcggtatcttcctcaacgatcaatatgtaaaaggtaaagccaaaaagctgaccaaagatattgagacgccgaaacaggcagccgtactgggcgcgggcattatgggcggcggtatcgcttaccagtcggcctggaaaggcgtaccggtcatcatgaaagatatcaacgataaatcgttgaacctcggcatgaccgaagcggccaagctgctgaacaaacagctggagcgcggcaagatcgacggtctgaagctggcgggcgtcatttcgaccatccacccgactctcgattatgccggtttcgatcgcgtagatgttgttgtcgaagcggtagtcgaaaatccgaaagtcaaaaaagcggtactggcggaaaccgagcagaaagtgcgcccggaaaccgtgctggcctcaaatacctccaccatcccaattggcgaactggcaagcgccctggaacgtccggaaaacttttgcggaatgcacttcttcaacccggtacaccggatgccgctggttgaaatcattcgcggcgagaagagttcagacgaaaccattgccaaagtcgtcgcctgggccagcaaaatgggcaagacaccgattgtagtcaacgactgccccggcttctttgttaaccgcgtgctgttcccctatttcgccggtttcagtcaactgctgcgcgacggcgcggatttccgcaaagtcgataaagtgatggaaaaacagttcggctggccgatgggcccggcctatctgctcgacgtggtgggtattgataccgcccaccacgcgcaggcagtaatggcggcaggcttcccacagcgtatgcagaaagattaccgcgacgcgatcgacgcattgtttgacgccagccgttttgggcaaaaaaacggtctgggcttctggcgctataaagaagacagcaagggtaagccgaagaaagaagaagatgccgcggtggatgacctgctggcaagcgtcagccagccgaaacgcgacttcagcgacgacgagataatcgcccgtatgatgatcccgatgattaacgaagtggttcgctgtctggaagaaggcattatcgcaagcccggcggaagccgatatggcgctggtctacggcctgggcttccctccgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861042_sequence/1
type: Sequence
persistent identity: BBa K861042 sequence
display id: BBa_K861042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaacaggttgtcattgtcgatgctattcgcaccccgatgggccgttcgaagggcggcgcgtttcgcaacgtgcgagcagaagatctctccgcccacttaatgcgtagcctgctggcgcgtaatccgtcgcttacagcggcgaccctcgatgatatttactggggctgcgtacaacaaacgctggagcaaggcttcaacattgcccgtaacgccgcgctgctggcagaaattccccattcggtaccggcggtcaccgtcaaccgtctgtgtggttcctcgatgcaggcgttacacgatgcagcgcgaatgatcatgaccggcgatgcgcaggtttgtctggttggcggcgtggagcatatggggcacgtgccgatgagccacggcgtggattttcacccgggtctgagtcgcaacgtcgccaaagcggcagggatgatggggctaacggcggaaatgctctcccgcctgcacggcattagccgggaaatgcaggaccagttcgccgcgcgttctcacgctcgcgcctgggccgccacccagtctggcgcattcaaaacggagattatcccgactggcggtcatgatgcagacggcgtgttgaagcagtttaactacgatgaagtgatccgcccggaaaccacggtcgaagcgctatcaacgctgcgtccggcatttgatccggttagtggcacggtcacggcgggcacctcatccgcgctttccgatggcgcagccgccatgctggtaatgagcgaaagtcgtgcccgtgagctgggtctgaaacctcgcgcccgtattcgctcaatggcagtggtgggttgtgatccgtcaattatgggttacgggccggttccggcgtcaaaactggcgttgaaaaaagcgggactgtcagccagcgatatcgatgtgtttgagatgaacgaagcgtttgccgcacagatcctgccatgcattaaggatctgggattgatggagcagatagacgagaagatcaacctcaacggcggtgcgatcgcgcttggtcatccgctcggctgctccggagcacgtatcagcaccacgcttatcaacctgatggagcgcaaagacgcgcagtttggtctggcgacgatgtgtattggtctgggtcagggcatcgccacggtgtttgagcgggtttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z