LOCUS       BBa_K861061              945 bp    DNA     linear       23-Jan-2026
DEFINITION  Efficacy testing RFP generator of BBa_K861060 (PfadR)
ACCESSION   BBa_K861061
VERSION     BBa_K861061.1  
FEATURES             Location/Qualifiers
     misc_feature    33..76
                     /label=FadR tandem binding site
     promoter        1..76
                     /label=BBa_K861060
     misc_feature    89..92
                     /label=conserved
     RBS             85..96
                     /label=BBa_B0034
     CDS             103..777
                     /label=mrfp1
     misc_feature    784..808
                     /label=Help:Barcodes
     CDS             103..783
                     /label=BBa_E1010
     stem_loop       828..871
                     /label=stem_loop
     misc_feature    817..896
                     /label=BBa_B0010
     terminator      817..896
                     /label=BBa_B0010
     stem_loop       912..931
                     /label=T7 TE
     misc_feature    938..938
                     /label=stop
     polyA_site      932..945
                     /label=polya
     misc_feature    905..945
                     /label=BBa_B0012
     terminator      905..945
                     /label=BBa_B0012
     misc_feature    85..945
                     /label=BBa_I13507
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctggtc cgacctatac tctcgccact
       61 ggtctgattt ctaagatact agagaaagag gagaaatact agatggcttc ctccgaagac
      121 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
      181 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
      241 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
      301 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
      361 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
      421 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
      481 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
      541 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
      601 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
      661 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
      721 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
      781 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//