LOCUS       BBa_K861062             1840 bp    DNA     linear       20-May-2024
DEFINITION  PfadR with FadR overexpressed
ACCESSION   BBa_K861062
VERSION     BBa_K861062.1  
FEATURES             Location/Qualifiers
     misc_feature    33..76
                     /label=FadR tandem binding site
     promoter        1..76
                     /label=BBa_K861060
     misc_feature    89..92
                     /label=conserved
     RBS             85..96
                     /label=BBa_B0034
     CDS             103..777
                     /label=mrfp1
     misc_feature    784..808
                     /label=Help:Barcodes
     CDS             103..783
                     /label=BBa_E1010
     stem_loop       828..871
                     /label=stem_loop
     misc_feature    817..896
                     /label=BBa_B0010
     terminator      817..896
                     /label=BBa_B0010
     stem_loop       912..931
                     /label=T7 TE
     misc_feature    938..938
                     /label=stop
     polyA_site      932..945
                     /label=polya
     misc_feature    905..945
                     /label=BBa_B0012
     terminator      905..945
                     /label=BBa_B0012
     misc_feature    85..945
                     /label=BBa_I13507
     misc_feature    1..945
                     /label=BBa_K861061
     promoter        954..988
                     /label=BBa_J23116
     RBS             997..1011
                     /label=RBS-1\Strong
     RBS             1004..1008
                     /label=RBS
     misc_feature    997..1011
                     /label=BBa_B0030
     RBS             997..1011
                     /label=BBa_B0030
     CDS             1018..1737
                     /label=BBa_K861050
     terminator      1746..1840
                     /label=BBa_B0024
     misc_feature    954..1840
                     /label=BBa_K861052
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctggtc cgacctatac tctcgccact
       61 ggtctgattt ctaagatact agagaaagag gagaaatact agatggcttc ctccgaagac
      121 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
      181 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
      241 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
      301 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
      361 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
      421 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
      481 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
      541 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
      601 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
      661 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
      721 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
      781 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag
      961 ctagctcagt cctagggact atgctagcta ctagagatta aagaggagaa atactagatg
     1021 gtcattaagg cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg
     1081 aataaccgct tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt
     1141 ggcgtaacgc gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg
     1201 accattcaac atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat
     1261 atccttgaaa cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg
     1321 ctgtcggtgc gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc
     1381 gataaagcgc aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt
     1441 gccgagctgg attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac
     1501 ggtctgattc ttaacgggat gaaagggctg tatacgcgta ttggtcgtca ctatttcgcc
     1561 aatccggaag cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt
     1621 gaaggcgcgc acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag
     1681 atttggcacc ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatac
     1741 tagagaaata ataaaaaagc cggattaata atctggcttt ttatattctc tctctagtat
     1801 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
//