##gff-version 3
##sequence-region BBa_K861062 1 1840
BBa_K861062	.	engineered_region	1	945	.	+	0	ID=BBa_K861061;Name=BBa_K861061
BBa_K861062	.	engineered_region	85	945	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K861061
BBa_K861062	.	CDS	103	783	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K861062	.	engineered_tag	784	808	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K861062	.	CDS	103	777	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K861062	.	terminator	817	896	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K861062	.	engineered_region	817	896	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K861062	.	stem_loop	828	871	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K861062	.	ribosome_entry_site	85	96	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K861062	.	conserved_region	89	92	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K861062	.	terminator	905	945	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K861062	.	stop_codon	938	938	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K861062	.	polyA_site	932	945	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K861062	.	stem_loop	912	931	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K861062	.	engineered_region	905	945	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K861062	.	promoter	1	76	.	+	0	ID=BBa_K861060;Name=BBa_K861060;Parent=BBa_K861061
BBa_K861062	.	non_covalent_binding_site	33	76	.	+	0	ID=annotation2183423;Name=FadR tandem binding site;Parent=BBa_K861060
BBa_K861062	.	engineered_region	954	1840	.	+	0	ID=BBa_K861052;Name=BBa_K861052
BBa_K861062	.	ribosome_entry_site	997	1011	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K861052
BBa_K861062	.	ribosome_entry_site	1004	1008	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K861062	.	engineered_region	997	1011	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K861062	.	ribosome_entry_site	997	1011	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K861062	.	CDS	1018	1737	.	+	0	ID=BBa_K861050;Name=BBa_K861050;Parent=BBa_K861052
BBa_K861062	.	promoter	954	988	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K861052
BBa_K861062	.	terminator	1746	1840	.	+	0	ID=BBa_B0024;Name=BBa_B0024;Parent=BBa_K861052
>BBa_K861062
tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatac
tagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatgga
aggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaa
ctgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagctt
acgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaa
cttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactg
cgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgt
acccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagt
taaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcc
cacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgat
agtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagttgacagctagctcagtcctagggactatgctagctactagagattaaagaggagaaatactagatggtcatta
aggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactat
tttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggca
cgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatcc
ttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttc
cactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggcc
gatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacg
gtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtct
ggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgc
tatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataat
actagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcc
cacccgaaggtgagccagtgtga