BBa_K861062
type: ComponentDefinition
persistent identity: BBa K861062
display id: BBa_K861062
version: 1
was generated by: iGEM to SBOL conversion
description: PfadR with FadR overexpressed
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K861052
component: BBa_K861061
sequence annotation: BBa_K861061
sequence annotation: BBa_K861052
sequence: https://synbiohub.org/public/igem/BBa_K861062_sequence/1

BBa_K861052
type: Component
persistent identity: component2183444
display id: component2183444
version: 1
top level: BBa_K861062
definition: BBa_K861052
access: public

BBa_K861061
type: Component
persistent identity: component2183437
display id: component2183437
version: 1
top level: BBa_K861062
definition: BBa_K861061
access: public

BBa_K861061
type: SequenceAnnotation
persistent identity: annotation2183437
display id: annotation2183437
version: 1
top level: BBa_K861062
location: https://synbiohub.org/public/igem/BBa_K861062/annotation2183437/range2183437/1
component: BBa_K861061

https://synbiohub.org/public/igem/BBa_K861062/annotation2183437/range2183437/1
type: Range
persistent identity: range2183437
display id: range2183437
version: 1
top level: BBa_K861062
direction: direction/reverse
start: 1
end: 945
orientation: inline

BBa_K861052
type: SequenceAnnotation
persistent identity: annotation2183444
display id: annotation2183444
version: 1
top level: BBa_K861062
location: https://synbiohub.org/public/igem/BBa_K861062/annotation2183444/range2183444/1
component: BBa_K861052

https://synbiohub.org/public/igem/BBa_K861062/annotation2183444/range2183444/1
type: Range
persistent identity: range2183444
display id: range2183444
version: 1
top level: BBa_K861062
direction: direction/reverse
start: 954
end: 1840
orientation: inline

BBa_K861052
type: ComponentDefinition
persistent identity: BBa K861052
display id: BBa_K861052
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive expressed FadR
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0024
component: BBa_K861050
component: BBa_B0030
component: BBa_J23116
sequence annotation: BBa_J23116
sequence annotation: BBa_B0030
sequence annotation: BBa_K861050
sequence annotation: BBa_B0024
sequence: https://synbiohub.org/public/igem/BBa_K861052_sequence/1

BBa_B0024
type: Component
persistent identity: component2182254
display id: component2182254
version: 1
top level: BBa_K861052
definition: BBa_B0024
access: public

BBa_K861050
type: Component
persistent identity: component2182253
display id: component2182253
version: 1
top level: BBa_K861052
definition: BBa_K861050
access: public

BBa_B0030
type: Component
persistent identity: component2182251
display id: component2182251
version: 1
top level: BBa_K861052
definition: BBa_B0030
access: public

BBa_J23116
type: Component
persistent identity: component2182249
display id: component2182249
version: 1
top level: BBa_K861052
definition: BBa_J23116
access: public

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2182249
display id: annotation2182249
version: 1
top level: BBa_K861052
location: https://synbiohub.org/public/igem/BBa_K861052/annotation2182249/range2182249/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K861052/annotation2182249/range2182249/1
type: Range
persistent identity: range2182249
display id: range2182249
version: 1
top level: BBa_K861052
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2182251
display id: annotation2182251
version: 1
top level: BBa_K861052
location: https://synbiohub.org/public/igem/BBa_K861052/annotation2182251/range2182251/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K861052/annotation2182251/range2182251/1
type: Range
persistent identity: range2182251
display id: range2182251
version: 1
top level: BBa_K861052
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K861050
type: SequenceAnnotation
persistent identity: annotation2182253
display id: annotation2182253
version: 1
top level: BBa_K861052
location: https://synbiohub.org/public/igem/BBa_K861052/annotation2182253/range2182253/1
component: BBa_K861050

https://synbiohub.org/public/igem/BBa_K861052/annotation2182253/range2182253/1
type: Range
persistent identity: range2182253
display id: range2182253
version: 1
top level: BBa_K861052
direction: direction/reverse
start: 65
end: 784
orientation: inline

BBa_B0024
type: SequenceAnnotation
persistent identity: annotation2182254
display id: annotation2182254
version: 1
top level: BBa_K861052
location: https://synbiohub.org/public/igem/BBa_K861052/annotation2182254/range2182254/1
component: BBa_B0024

https://synbiohub.org/public/igem/BBa_K861052/annotation2182254/range2182254/1
type: Range
persistent identity: range2182254
display id: range2182254
version: 1
top level: BBa_K861052
direction: direction/reverse
start: 793
end: 887
orientation: inline

BBa_K861061
type: ComponentDefinition
persistent identity: BBa K861061
display id: BBa_K861061
version: 1
was generated by: iGEM to SBOL conversion
description: Efficacy testing RFP generator of BBa_K861060 (PfadR)
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: Promoter + BBa_I13507 in high copy number plasmid. Used to amplify plasmid
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K861060
sequence annotation: BBa_K861060
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K861061_sequence/1

BBa_I13507
type: Component
persistent identity: component2182268
display id: component2182268
version: 1
top level: BBa_K861061
definition: BBa_I13507
access: public

BBa_K861060
type: Component
persistent identity: component2182256
display id: component2182256
version: 1
top level: BBa_K861061
definition: PfadR
access: public

BBa_K861060
type: SequenceAnnotation
persistent identity: annotation2182256
display id: annotation2182256
version: 1
top level: BBa_K861061
location: https://synbiohub.org/public/igem/BBa_K861061/annotation2182256/range2182256/1
component: BBa_K861060

https://synbiohub.org/public/igem/BBa_K861061/annotation2182256/range2182256/1
type: Range
persistent identity: range2182256
display id: range2182256
version: 1
top level: BBa_K861061
direction: direction/reverse
start: 1
end: 76
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2182268
display id: annotation2182268
version: 1
top level: BBa_K861061
location: https://synbiohub.org/public/igem/BBa_K861061/annotation2182268/range2182268/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K861061/annotation2182268/range2182268/1
type: Range
persistent identity: range2182268
display id: range2182268
version: 1
top level: BBa_K861061
direction: direction/reverse
start: 85
end: 945
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011), reversed
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_K861050
type: ComponentDefinition
persistent identity: BBa K861050
display id: BBa_K861050
version: 1
was generated by: iGEM to SBOL conversion
description: FadR, fatty acid sensor from E.coli str. K12
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: E.coli str.K12 lab mutant DH5α
owned by: james
owned by: myers
mutable description: FadR is a transcriptional regulator,which, when binding to acyl-CoA can either serve as activator for fatty acid synthetase gene like FabA, FabB and etc. or repressor for fatty acid degradation gene like FadA, FadB, FadD and etc. After long chain fatty acid is converted to fatty acyl- CoA by FadD, it can bind to FadR. This binding will alter the conformation of FadR and FadR can no longer bind to the DNA sequence it recognizes to fulfill its function. To our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors.
discontinued: false
dominant: true
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K861050_sequence/1

PfadR
type: ComponentDefinition
persistent identity: BBa K861060
display id: BBa_K861060
version: 1
was generated by: iGEM to SBOL conversion
description: PfadR, synthetic promoter with tandem FadR binding site
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:35Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap. We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1121_
m user id: 0
owner id: 12357
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: false
creator: Kuanwei Sheng
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FadR tandem binding site
sequence: https://synbiohub.org/public/igem/BBa_K861060_sequence/1

FadR tandem binding site
type: SequenceAnnotation
persistent identity: annotation2183423
display id: annotation2183423
version: 1
top level: PfadR
location: https://synbiohub.org/public/igem/BBa_K861060/annotation2183423/range2183423/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K861060/annotation2183423/range2183423/1
type: Range
persistent identity: range2183423
display id: range2183423
version: 1
top level: PfadR
direction: direction/forward
start: 33
end: 76
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K861062_sequence/1
type: Sequence
persistent identity: BBa K861062 sequence
display id: BBa_K861062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagagattaaagaggagaaatactagatggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861052_sequence/1
type: Sequence
persistent identity: BBa K861052 sequence
display id: BBa_K861052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagctactagagattaaagaggagaaatactagatggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861061_sequence/1
type: Sequence
persistent identity: BBa K861061 sequence
display id: BBa_K861061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0024_sequence/1
type: Sequence
persistent identity: BBa B0024 sequence
display id: BBa_B0024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861050_sequence/1
type: Sequence
persistent identity: BBa K861050 sequence
display id: BBa_K861050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K861060_sequence/1
type: Sequence
persistent identity: BBa K861060 sequence
display id: BBa_K861060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z