BBa_J23107
1
BBa_J23107
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
false
_52_
0
483
95
It's complicated
true
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K861070
1
BBa_K861070
AdrA gene from E.coli K12
2012-09-17T11:00:00Z
2015-05-08T01:13:35Z
E.coli K12 Dh5α
This is the coding sequence of gene AdrA, which contain a GGDEF domain to catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria.
false
false
_1121_
0
11007
9
Not in stock
true
None
false
Kuanwei Sheng
BBa_B0024
1
BBa_B0024
double terminator (B0012-B0011), reversed
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy
BBa_K861071
1
BBa_K861071
AdrA gene with a RBS and a terminator
2012-09-17T11:00:00Z
2015-05-08T01:13:35Z
E.coli K12 DH5α
AdrA protein contain a GGDEF domain to catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. This part contain coding sequence of AdrA with a RBS and a terminator
false
false
_1121_
0
11007
9
It's complicated
false
None
false
Kuanwei Sheng
component2191668
1
BBa_B0030
component2191671
1
BBa_B0024
component2191670
1
BBa_K861070
annotation2191670
1
BBa_K861070
range2191670
1
24
1190
annotation2191671
1
BBa_B0024
range2191671
1
1199
1293
annotation2191668
1
BBa_B0030
range2191668
1
1
15
BBa_K861073
1
BBa_K861073
Constitutively expressed AdrA
2012-09-20T11:00:00Z
2015-05-08T01:13:35Z
E.coli K12 DH5α
AdrA contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. This part was designed to characterize the effect of the gene AdrA on cell colonization.
false
false
_1121_
0
11007
9
It's complicated
false
None
false
Kuanwei Sheng
component2191700
1
BBa_K861071
component2191694
1
BBa_J23107
annotation2191694
1
BBa_J23107
range2191694
1
1
35
annotation2191700
1
BBa_K861071
range2191700
1
44
1336
BBa_K861070_sequence
1
ggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctga
BBa_B0024_sequence
1
aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_K861073_sequence
1
tttacggctagctcagccctaggtattatgctagctactagagattaaagaggagaaatactagagggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_J23107_sequence
1
tttacggctagctcagccctaggtattatgctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K861071_sequence
1
attaaagaggagaaatactagagggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z