BBa_K861070 1 BBa_K861070 AdrA gene from E.coli K12 2012-09-17T11:00:00Z 2015-05-08T01:13:35Z E.coli K12 Dh5&#945; This is the coding sequence of gene AdrA, which contain a GGDEF domain to catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. false false _1121_ 0 11007 9 Not in stock true None false Kuanwei Sheng BBa_K861074 1 BBa_K861074 Pcar regulated AdrA 2012-09-20T11:00:00Z 2015-05-08T01:13:35Z E.coli K12 DH5&#945; AdrA contains a GGDEF domain that can catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activate bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. This part was designed to characterize the effect of the gene AdrA on cellulose synthesis. false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2191724 1 BBa_K861071 component2191718 1 BBa_K861171 annotation2191724 1 BBa_K861071 range2191724 1 45 1337 annotation2191718 1 BBa_K861171 range2191718 1 1 36 BBa_K861171 1 Pcar Pcar, synthetic promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:36Z Escherichia coli str. K-12 substr. MG1655 This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183482 1 Consensus CRP binding site range2183482 1 15 36 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_K861071 1 BBa_K861071 AdrA gene with a RBS and a terminator 2012-09-17T11:00:00Z 2015-05-08T01:13:35Z E.coli K12 DH5&#945; AdrA protein contain a GGDEF domain to catalyze GTP into c-di-GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. This part contain coding sequence of AdrA with a RBS and a terminator false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2191670 1 BBa_K861070 component2191671 1 BBa_B0024 component2191668 1 BBa_B0030 annotation2191671 1 BBa_B0024 range2191671 1 1199 1293 annotation2191668 1 BBa_B0030 range2191668 1 1 15 annotation2191670 1 BBa_K861070 range2191670 1 24 1190 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K861070_sequence 1 ggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctga BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861171_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattt BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861071_sequence 1 attaaagaggagaaatactagagggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861074_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattttactagagattaaagaggagaaatactagagggctccgtctctataatttgggaaaattgtttctgaatgttcccaaaaataatgttcccaaaaataatgaatgatgaaaactttttcaaaaaagcggcggcgcacggggaggaacctcctttaactcctcaaaacgaacatcagcggtccgggctgcgcttcgcccgtcgcgtcagactaccccgtgcggttggcctggctggcatgttcttaccgattgcttcaacgctggtttcacacccgccgccgggctggtggtggctggtgttggtcggctgggcgttcgtctggccgcatttagcctggcagatagcgagcagggccgtcgatccgcttagccgggaaatttacaacttaaaaaccgatgcagtattagcgggaatgtgggtaggcgtaatgggcgtaaacgtgctgccttccaccgcgatgttgatgattatgtgtctgaatttgatgggggcaggcggcccccgtctgtttgtcgcgggtctggtgttgatggtggtttcctgccttgtcaccctcgagctgacgggcattaccgtgtcgttcaatagtgcgccgctggaatggtggctctcccttcccattattgtcatttatcctctgctgtttggctgggtcagctaccagacggcaaccaaactggcggaacataaacgcaggttgcaggtcatgagtacccgcgacggcatgacgggcgtgtataaccgacgtcattgggaaactatgttacgcaatgaatttgataactgtcggcggcataatcgcgatgcaacgttactgattatcgatatcgaccatttcaagagcatcaacgatacctggggccatgatgtgggcgatgaagcgattgtggcgcttacccgacagttacaaattaccctgcgcggtagcgatgtgattggtcggtttggcggcgatgagtttgcagtaatcatgtccggtacgccagctgagagcgccattaccgccatgttacgggtgcatgaagggctaaatacattacgtttgccgaatacgccacaggtaactttacggattagtgtgggggttgcgccgctgaacccacaaatgagtcactatcgtgagtggttgaaatcggcagatttggcgctttacaaagcaaagaaagccggacgtaaccgcaccgaagtggcggcctgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z