LOCUS BBa_K861101 2743 bp DNA linear 25-Nov-2024 DEFINITION BcsA with RBS and terminator ACCESSION BBa_K861101 VERSION BBa_K861101.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 CDS 22..2640 /label=BBa_K861100 terminator 2649..2743 /label=BBa_B0024 ORIGIN 1 attaaagagg agaaatacta gatgagtatc ctgacccggt ggttgcttat cccgccggtc 61 aacgcgcggc ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc 121 gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg 181 cgctggcagc gtattcgcgc agaacataaa aacctgtatc cgcatatcaa cgcctcgcgt 241 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca 301 tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca aaatattcgt 361 ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat 421 ctggatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt 481 atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc gtttaacccg 541 ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt acggcggatg 601 ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat 661 atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg 721 cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag 781 gtagtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc gctgtggccg 841 tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa aaataccatt 901 tacgcctcgc tgggtatcga ctggccgaaa gataagctga atatctggat ccttgatgac 961 ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgc 1021 accactcatg aacatgcgaa agcaggcaac atcaacaatg cgctgaaata tgccaaaggc 1081 gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttctt gcaaatgacc 1141 atgggctggt tcctgaaaga aaaacagctg gcgatgatgc agacgccgca ccacttcttc 1201 tcaccggacc cgtttgaacg caacctgggg cgtttccgta aaacgccgaa cgaaggcacg 1261 ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt 1321 tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg gcggcattgc tgtcgaaacc 1381 gtgactgaag atgcgcatac ttctctgcgg ttgcaccgtc gtggctatac ctccgcgtat 1441 atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag 1501 cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc tcgataaccc gctcaccggt 1561 aaagggctga agtttgctca gcggctatgt tacgtcaacg ccatgttcca cttcttgtcg 1621 ggcattccac ggctgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac 1681 atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc tgccgcatat gatccatgcc 1741 agcctgacca actccaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac 1801 gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac 1861 aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg 1921 gtgatctcgc ggccctacat cttccttgtc ctgctcaacc tggtgggcgt tgcggtaggc 1981 atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg 2041 tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa 2101 caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat 2161 ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc 2221 aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag 2281 caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa 2341 ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg 2401 gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg 2461 gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg 2521 gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg 2581 cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga 2641 tactagagaa ataataaaaa agccggatta ataatctggc tttttatatt ctctctctag 2701 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tga //