LOCUS       BBa_K861101             2743 bp    DNA     linear       26-Dec-2024
DEFINITION  BcsA with RBS and terminator
ACCESSION   BBa_K861101
VERSION     BBa_K861101.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..2640
                     /label=BBa_K861100
     terminator      2649..2743
                     /label=BBa_B0024
ORIGIN
        1 attaaagagg agaaatacta gatgagtatc ctgacccggt ggttgcttat cccgccggtc
       61 aacgcgcggc ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc
      121 gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg
      181 cgctggcagc gtattcgcgc agaacataaa aacctgtatc cgcatatcaa cgcctcgcgt
      241 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca
      301 tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca aaatattcgt
      361 ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat
      421 ctggatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt
      481 atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc gtttaacccg
      541 ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt acggcggatg
      601 ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat
      661 atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg
      721 cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag
      781 gtagtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc gctgtggccg
      841 tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa aaataccatt
      901 tacgcctcgc tgggtatcga ctggccgaaa gataagctga atatctggat ccttgatgac
      961 ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgc
     1021 accactcatg aacatgcgaa agcaggcaac atcaacaatg cgctgaaata tgccaaaggc
     1081 gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttctt gcaaatgacc
     1141 atgggctggt tcctgaaaga aaaacagctg gcgatgatgc agacgccgca ccacttcttc
     1201 tcaccggacc cgtttgaacg caacctgggg cgtttccgta aaacgccgaa cgaaggcacg
     1261 ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt
     1321 tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg gcggcattgc tgtcgaaacc
     1381 gtgactgaag atgcgcatac ttctctgcgg ttgcaccgtc gtggctatac ctccgcgtat
     1441 atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag
     1501 cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc tcgataaccc gctcaccggt
     1561 aaagggctga agtttgctca gcggctatgt tacgtcaacg ccatgttcca cttcttgtcg
     1621 ggcattccac ggctgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac
     1681 atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc tgccgcatat gatccatgcc
     1741 agcctgacca actccaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac
     1801 gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac
     1861 aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg
     1921 gtgatctcgc ggccctacat cttccttgtc ctgctcaacc tggtgggcgt tgcggtaggc
     1981 atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg
     2041 tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa
     2101 caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat
     2161 ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc
     2221 aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag
     2281 caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa
     2341 ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg
     2401 gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg
     2461 gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg
     2521 gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg
     2581 cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga
     2641 tactagagaa ataataaaaa agccggatta ataatctggc tttttatatt ctctctctag
     2701 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tga
//