BBa_K861101 1 BBa_K861101 BcsA with RBS and terminator 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsA, BcsB, BcsZ and BcsC are involved in biosynthesis of cellulose, a characteristic of the rdar morphotype. BcsA is considered to be the catalytic subunit for cellulose biosynthesis. The cellulose synthase is located in the cytoplasmic membrane with 8 to 10 predicted transmembrane domains.Besides the high homology to other processive glycosyltransferases, BcsA has been experimentally shown to bind the substrate UDP-glucose.This device contains BcsA with a RBS and a terminator. false false _1121_ 0 12357 9 It's complicated false None false Xian Xia component2181942 1 BBa_B0024 component2181939 1 BBa_B0030 component2181941 1 BBa_K861100 annotation2181942 1 BBa_B0024 range2181942 1 2649 2743 annotation2181941 1 BBa_K861100 range2181941 1 22 2640 annotation2181939 1 BBa_B0030 range2181939 1 1 15 BBa_K861102 1 BBa_K861102 BcsA controlled by promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsA is controlled by promoter PII which can be repressed by CRP-cAMP complex.So the expression of catalytic subunit of cellulose synthetase will be repressed at high concentration of CRP-cAMP complex.On the contrary,BcsA can be expressed at low concentration of CRP-cAMP complex. false false _1121_ 0 12357 9 Not in stock false None false Xian Xia component2181979 1 BBa_K861101 component2181973 1 BBa_K861171 annotation2181973 1 BBa_K861171 range2181973 1 1 36 annotation2181979 1 BBa_K861101 range2181979 1 45 2787 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K861171 1 Pcar Pcar, synthetic promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:36Z Escherichia coli str. K-12 substr. MG1655 This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183482 1 Consensus CRP binding site range2183482 1 15 36 BBa_K861100 1 BBa_K861100 BcsA,cellulose synthetase, catalytic subunit 2012-09-06T11:00:00Z 2015-06-17T01:21:19Z Escherichia coli str. K-12 substr. MG1655 BcsA, BcsB, BcsZ and BcsC are involved in biosynthesis of cellulose, a characteristic of the rdar morphotype. BcsA is considered to be the catalytic subunit for cellulose biosynthesis. The cellulose synthase is located in the cytoplasmic membrane with 8 to 10 predicted transmembrane domains.Besides the high homology to other processive glycosyltransferases, BcsA has been experimentally shown to bind the substrate UDP-glucose. false false _1121_ 0 4206 12357 9 In stock false None false Xian Xia BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861171_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattt BBa_K861101_sequence 1 attaaagaggagaaatactagatgagtatcctgacccggtggttgcttatcccgccggtcaacgcgcggcttatcgggcgttatcgcgattatcgtcgtcacggtgcgtcggctttcagcgcgacgctcggctgtttctggatgatcctggcctggatttttattccgctggagcacccgcgctggcagcgtattcgcgcagaacataaaaacctgtatccgcatatcaacgcctcgcgtccgcgtccgctggacccggtccgttatctcattcaaacatgctggttattgatcggtgcatcgcgcaaagaaacgccgaaaccgcgcaggcgggcattttcaggtctgcaaaatattcgtggacgttaccatcaatggatgaacgagctgcctgagcgcgttagccataaaacacagcatctggatgagaaaaaagagctcggtcatttgagtgccggggcgcggcggttgatcctcggtatcatcgtcaccttctcgctgattctggcgttaatctgcgttactcagccgtttaacccgctggcgcagtttatcttcctgatgctgctgtggggggtagcgctgatcgtacggcggatgccggggcgcttctcggcgctaatgttgattgtgctgtcgctgaccgtttcttgccgttatatctggtggcgttacacctctacgctgaactgggacgatccggtcagcctggtgtgcgggcttattctgctcttcgctgaaacgtacgcgtggattgtgctggtgctcggctacttccaggtagtatggccgctgaatcgtcagccggtgccattgccgaaagatatgtcgctgtggccgtcggtggatatctttgtcccgacttacaacgaagatctcaacgtggtgaaaaataccatttacgcctcgctgggtatcgactggccgaaagataagctgaatatctggatccttgatgacggcggcagggaagagtttcgccagtttgcgcaaaacgtgggggtgaaatatatcgcccgcaccactcatgaacatgcgaaagcaggcaacatcaacaatgcgctgaaatatgccaaaggcgagttcgtgtcgattttcgactgcgaccacgtaccaacgcgatcgttcttgcaaatgaccatgggctggttcctgaaagaaaaacagctggcgatgatgcagacgccgcaccacttcttctcaccggacccgtttgaacgcaacctggggcgtttccgtaaaacgccgaacgaaggcacgctgttctatggtctggtgcaggatggcaacgatatgtgggacgccactttcttctgcggttcctgtgcggtgattcgtcgtaagccgctggatgaaattggcggcattgctgtcgaaaccgtgactgaagatgcgcatacttctctgcggttgcaccgtcgtggctatacctccgcgtatatgcgtattccgcaggcggcggggctggcgaccgaaagtctgtcggcgcatatcggtcagcgtattcgctgggcgcgcgggatggtacaaatcttccgtctcgataacccgctcaccggtaaagggctgaagtttgctcagcggctatgttacgtcaacgccatgttccacttcttgtcgggcattccacggctgatcttcctgactgcgccgctggcgttcctgctgcttcatgcctacatcatctatgcgccagcgttgatgatcgccctattcgtgctgccgcatatgatccatgccagcctgaccaactccaagatccagggcaaatatcgccactctttctggagtgaaatctacgaaacggtgctggcgtggtatatcgcaccaccgacgctggtggcgctgattaacccgcacaaaggcaaatttaacgtcaccgccaaaggtggactggtggaagaagagtacgtcgactgggtgatctcgcggccctacatcttccttgtcctgctcaacctggtgggcgttgcggtaggcatctggcgctacttctatggcccgccaaccgagatgctcaccgtggtcgtcagtatggtgtgggtgttctacaacctgattgttcttggcggcgcagttgcggtatcggtagaaagcaaacaggtacgccgatcgcaccgcgtggagatgacgatgcccgcggcaattgcccgcgaagatggtcacctcttctcgtgtaccgttcaggatttctccgacggtggtttggggatcaagatcaacggtcaggcgcagattctggaagggcagaaagtgaatctgttgcttaaacgcggtcagcaggaatacgtcttcccgacccaggtggcgcgcgtgatgggtaatgaagttgggctgaaattaatgccgctcaccacccagcaacatatcgattttgtgcagtgtacgtttgcccgtgcggatacatgggcgctctggcaggacagctacccggaagataagccgctggaaagtctgctggatattctgaagctcggcttccgtggctaccgccatctggcggagtttgcgccttcttcggtgaagggcatattccgtgtgctgacttctctggtttcctgggttgtatcgtttattccgcgccgcccggagcggagcgaaacggcacaaccatcggatcaggctttggctcaacaatgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861102_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattttactagagattaaagaggagaaatactagatgagtatcctgacccggtggttgcttatcccgccggtcaacgcgcggcttatcgggcgttatcgcgattatcgtcgtcacggtgcgtcggctttcagcgcgacgctcggctgtttctggatgatcctggcctggatttttattccgctggagcacccgcgctggcagcgtattcgcgcagaacataaaaacctgtatccgcatatcaacgcctcgcgtccgcgtccgctggacccggtccgttatctcattcaaacatgctggttattgatcggtgcatcgcgcaaagaaacgccgaaaccgcgcaggcgggcattttcaggtctgcaaaatattcgtggacgttaccatcaatggatgaacgagctgcctgagcgcgttagccataaaacacagcatctggatgagaaaaaagagctcggtcatttgagtgccggggcgcggcggttgatcctcggtatcatcgtcaccttctcgctgattctggcgttaatctgcgttactcagccgtttaacccgctggcgcagtttatcttcctgatgctgctgtggggggtagcgctgatcgtacggcggatgccggggcgcttctcggcgctaatgttgattgtgctgtcgctgaccgtttcttgccgttatatctggtggcgttacacctctacgctgaactgggacgatccggtcagcctggtgtgcgggcttattctgctcttcgctgaaacgtacgcgtggattgtgctggtgctcggctacttccaggtagtatggccgctgaatcgtcagccggtgccattgccgaaagatatgtcgctgtggccgtcggtggatatctttgtcccgacttacaacgaagatctcaacgtggtgaaaaataccatttacgcctcgctgggtatcgactggccgaaagataagctgaatatctggatccttgatgacggcggcagggaagagtttcgccagtttgcgcaaaacgtgggggtgaaatatatcgcccgcaccactcatgaacatgcgaaagcaggcaacatcaacaatgcgctgaaatatgccaaaggcgagttcgtgtcgattttcgactgcgaccacgtaccaacgcgatcgttcttgcaaatgaccatgggctggttcctgaaagaaaaacagctggcgatgatgcagacgccgcaccacttcttctcaccggacccgtttgaacgcaacctggggcgtttccgtaaaacgccgaacgaaggcacgctgttctatggtctggtgcaggatggcaacgatatgtgggacgccactttcttctgcggttcctgtgcggtgattcgtcgtaagccgctggatgaaattggcggcattgctgtcgaaaccgtgactgaagatgcgcatacttctctgcggttgcaccgtcgtggctatacctccgcgtatatgcgtattccgcaggcggcggggctggcgaccgaaagtctgtcggcgcatatcggtcagcgtattcgctgggcgcgcgggatggtacaaatcttccgtctcgataacccgctcaccggtaaagggctgaagtttgctcagcggctatgttacgtcaacgccatgttccacttcttgtcgggcattccacggctgatcttcctgactgcgccgctggcgttcctgctgcttcatgcctacatcatctatgcgccagcgttgatgatcgccctattcgtgctgccgcatatgatccatgccagcctgaccaactccaagatccagggcaaatatcgccactctttctggagtgaaatctacgaaacggtgctggcgtggtatatcgcaccaccgacgctggtggcgctgattaacccgcacaaaggcaaatttaacgtcaccgccaaaggtggactggtggaagaagagtacgtcgactgggtgatctcgcggccctacatcttccttgtcctgctcaacctggtgggcgttgcggtaggcatctggcgctacttctatggcccgccaaccgagatgctcaccgtggtcgtcagtatggtgtgggtgttctacaacctgattgttcttggcggcgcagttgcggtatcggtagaaagcaaacaggtacgccgatcgcaccgcgtggagatgacgatgcccgcggcaattgcccgcgaagatggtcacctcttctcgtgtaccgttcaggatttctccgacggtggtttggggatcaagatcaacggtcaggcgcagattctggaagggcagaaagtgaatctgttgcttaaacgcggtcagcaggaatacgtcttcccgacccaggtggcgcgcgtgatgggtaatgaagttgggctgaaattaatgccgctcaccacccagcaacatatcgattttgtgcagtgtacgtttgcccgtgcggatacatgggcgctctggcaggacagctacccggaagataagccgctggaaagtctgctggatattctgaagctcggcttccgtggctaccgccatctggcggagtttgcgccttcttcggtgaagggcatattccgtgtgctgacttctctggtttcctgggttgtatcgtttattccgcgccgcccggagcggagcgaaacggcacaaccatcggatcaggctttggctcaacaatgatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861100_sequence 1 atgagtatcctgacccggtggttgcttatcccgccggtcaacgcgcggcttatcgggcgttatcgcgattatcgtcgtcacggtgcgtcggctttcagcgcgacgctcggctgtttctggatgatcctggcctggatttttattccgctggagcacccgcgctggcagcgtattcgcgcagaacataaaaacctgtatccgcatatcaacgcctcgcgtccgcgtccgctggacccggtccgttatctcattcaaacatgctggttattgatcggtgcatcgcgcaaagaaacgccgaaaccgcgcaggcgggcattttcaggtctgcaaaatattcgtggacgttaccatcaatggatgaacgagctgcctgagcgcgttagccataaaacacagcatctggatgagaaaaaagagctcggtcatttgagtgccggggcgcggcggttgatcctcggtatcatcgtcaccttctcgctgattctggcgttaatctgcgttactcagccgtttaacccgctggcgcagtttatcttcctgatgctgctgtggggggtagcgctgatcgtacggcggatgccggggcgcttctcggcgctaatgttgattgtgctgtcgctgaccgtttcttgccgttatatctggtggcgttacacctctacgctgaactgggacgatccggtcagcctggtgtgcgggcttattctgctcttcgctgaaacgtacgcgtggattgtgctggtgctcggctacttccaggtagtatggccgctgaatcgtcagccggtgccattgccgaaagatatgtcgctgtggccgtcggtggatatctttgtcccgacttacaacgaagatctcaacgtggtgaaaaataccatttacgcctcgctgggtatcgactggccgaaagataagctgaatatctggatccttgatgacggcggcagggaagagtttcgccagtttgcgcaaaacgtgggggtgaaatatatcgcccgcaccactcatgaacatgcgaaagcaggcaacatcaacaatgcgctgaaatatgccaaaggcgagttcgtgtcgattttcgactgcgaccacgtaccaacgcgatcgttcttgcaaatgaccatgggctggttcctgaaagaaaaacagctggcgatgatgcagacgccgcaccacttcttctcaccggacccgtttgaacgcaacctggggcgtttccgtaaaacgccgaacgaaggcacgctgttctatggtctggtgcaggatggcaacgatatgtgggacgccactttcttctgcggttcctgtgcggtgattcgtcgtaagccgctggatgaaattggcggcattgctgtcgaaaccgtgactgaagatgcgcatacttctctgcggttgcaccgtcgtggctatacctccgcgtatatgcgtattccgcaggcggcggggctggcgaccgaaagtctgtcggcgcatatcggtcagcgtattcgctgggcgcgcgggatggtacaaatcttccgtctcgataacccgctcaccggtaaagggctgaagtttgctcagcggctatgttacgtcaacgccatgttccacttcttgtcgggcattccacggctgatcttcctgactgcgccgctggcgttcctgctgcttcatgcctacatcatctatgcgccagcgttgatgatcgccctattcgtgctgccgcatatgatccatgccagcctgaccaactccaagatccagggcaaatatcgccactctttctggagtgaaatctacgaaacggtgctggcgtggtatatcgcaccaccgacgctggtggcgctgattaacccgcacaaaggcaaatttaacgtcaccgccaaaggtggactggtggaagaagagtacgtcgactgggtgatctcgcggccctacatcttccttgtcctgctcaacctggtgggcgttgcggtaggcatctggcgctacttctatggcccgccaaccgagatgctcaccgtggtcgtcagtatggtgtgggtgttctacaacctgattgttcttggcggcgcagttgcggtatcggtagaaagcaaacaggtacgccgatcgcaccgcgtggagatgacgatgcccgcggcaattgcccgcgaagatggtcacctcttctcgtgtaccgttcaggatttctccgacggtggtttggggatcaagatcaacggtcaggcgcagattctggaagggcagaaagtgaatctgttgcttaaacgcggtcagcaggaatacgtcttcccgacccaggtggcgcgcgtgatgggtaatgaagttgggctgaaattaatgccgctcaccacccagcaacatatcgattttgtgcagtgtacgtttgcccgtgcggatacatgggcgctctggcaggacagctacccggaagataagccgctggaaagtctgctggatattctgaagctcggcttccgtggctaccgccatctggcggagtttgcgccttcttcggtgaagggcatattccgtgtgctgacttctctggtttcctgggttgtatcgtttattccgcgccgcccggagcggagcgaaacggcacaaccatcggatcaggctttggctcaacaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z