BBa_K861111 1 BBa_K861111 BcsB with RBS and terminator 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsB is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix observed during multicellular morphotype (rdar) growth. The BcsB protein that was indirectly inferred to bind c-di-GMP.This device contains BcsB with a RBS and a terminator. false false _1121_ 0 12357 9 It's complicated false None. false Xian Xia component2181944 1 BBa_B0030 component2181946 1 BBa_K861110 component2181947 1 BBa_B0024 annotation2181946 1 BBa_K861110 range2181946 1 22 2361 annotation2181944 1 BBa_B0030 range2181944 1 1 15 annotation2181947 1 BBa_B0024 range2181947 1 2370 2464 BBa_K861112 1 BBa_K861112 BcsB controlled by promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsB is controlled by promoter PII which can be repressed by CRP-cAMP complex.So the expression of BcsB will be repressed at high concentration of CRP-cAMP complex.On the contrary,BcsB can be expressed at low concentration of CRP-cAMP complex. false false _1121_ 0 12357 9 Not in stock false None false Xian Xia component2181986 1 BBa_K861111 component2181980 1 BBa_K861171 annotation2181986 1 BBa_K861111 range2181986 1 45 2508 annotation2181980 1 BBa_K861171 range2181980 1 1 36 BBa_K861110 1 BBa_K861110 BcsB,regulator of cellulose synthetase 2012-09-06T11:00:00Z 2015-06-17T01:21:30Z Escherichia coli str. K-12 substr. MG1655 Released HQ 2013 BcsB is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix observed during multicellular morphotype (rdar) growth. The BcsB protein that was indirectly inferred to bind c-di-GMP. false false _1121_ 0 4206 12357 9 In stock false None false Xian Xia BBa_K861171 1 Pcar Pcar, synthetic promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:36Z Escherichia coli str. K-12 substr. MG1655 This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183482 1 Consensus CRP binding site range2183482 1 15 36 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861171_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattt BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861111_sequence 1 attaaagaggagaaatactagatgaaaagaaaactattctggatttgtgcagtggctatggggatgagtgcgttcccctctttcatgacgcaggcgacgccagcaacgcaaccactgatcaatgctgagccagctgtagccgcccagacggaacaaaatccgcaggtggggcaagtgatgccgggcgtgcagggcgctgatgcgccagtcgtggcgcagaacggtccttcgcgtgatgtgaagctgacctttgcgcaaattgcaccgccgccgggcagcatggtgctacgtggcattaacccgaacggcagcattgagtttggtatgcgcagcgatgaagtggtgacgaaggcgatgctcaacctcgaatacaccccatcgccatcgttactgcctgtccagtcgcagttaaaggtttatctcaatgatgaactgatgggcgtgctgccagtgaccaaagaacagttgggtaaaaaaacgctggcgcaaatgcccattaacccactgtttattagcgacttcaaccgtgtacggctggagtttgtcggccattatcaggacgtgtgcgaaaaaccggccagcaccacgctttggctggatgttgggcggagcagtggactggatctgacctatcagaccctgaatgtgaagaatgacctgtcacacttcccggtgccattctttgacccgagcgataaccgcaccaacaccttgccgatggtctttgcgggtgcgccggatgttgggctgcaacaagcctctgccattgtcgcctcgtggtttggttcgcgttctggctggcgtgggcagaacttcccggtactctataaccaactgccggatcgcaatgccattgtctttgcaaccaacgacaaacggccggacttcctgcgcgatcatccggcggtaaaagccccggtgattgagatgattaaccatccgcagaatccttacgtcaaactgctggtggtgtttggtcgtgacgacaaagacctgttgcaggcagcgaaaggtatcgctcagggtaacattctgttccgtggtgaaagcgtggtagtgaatgaagtgaaaccgctgctaccgcgtaagccgtacgatgcgccgaactgggtacgtaccgatcgtccggtcacatttggcgaactgaaaacctatgaagaacagttacaatccagcggtcttgagccagcagcgattaacgtttcgctaaacctgccgccggatctctacctgatgcgcagtaccggcattgatatggatattaattaccgctacaccatgccgccggtgaaagacagttcgcggatggatatcagcctgaataaccagttcctgcaatccttcaacctgagcagcaaacaggaggcgaaccgcctgctgctgcggattccggtattacaaggtttgctggatggcaaaacagatgtctctattccggcgctgaaactgggcgcgaccaaccagctgcgcttcgactttgagtatatgaacccgatgccgggcggttcggtggataactgtattaccttccagccggtgcagaatcatgtggtgattggtgacgactccaccatcgacttctcgaagtattaccacttcatcccgatgccggatctacgcgcctttgctaacgcgggcttcccattcagccggatggcggatctgtcgcaaaccatcaccgtgatgccgaaagcgcctaacgaagcacagatggaaacgttgctgaatactgttggttttatcggcgcacagacgggcttcccggcgattaatctgacggtgaccgatgatggcagcaccattcagggcaaagatgccgacatcatgatcatcggtggtatcccggacaaactgaaagacgataagcagatcgacctattggtgcaggcgaccgaaagctgggtgaaaacaccgatgcgccagaccccgttccccggcattgtgccggacgagagcgatcgcgcggcagaaacccggtcaacgctgacctcttccggtgcgatggcggcggtgattggcttccagtcgccgtataacgaccagcgcagcgtgattgcgctgttggcagatagcccacgcggttatgaaatgcttaacgatgcggtgaacgatagcggcaaacgcgccaccatgttcggttcggtcgcggtgatccgcgagtccggtatcaacagcctacgtgttggcgacgtttattacgtaggtcatctgccgtggttcgagcgcgtgtggtatgcgctggcaaaccatccgattctgctggcggtgctggcggctatcagtgtgatattgctggcatgggtactgtggcgtctgctgcgaattattagtcgtcgtcgtcttaacccggataacgagtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861110_sequence 1 atgaaaagaaaactattctggatttgtgcagtggctatggggatgagtgcgttcccctctttcatgacgcaggcgacgccagcaacgcaaccactgatcaatgctgagccagctgtagccgcccagacggaacaaaatccgcaggtggggcaagtgatgccgggcgtgcagggcgctgatgcgccagtcgtggcgcagaacggtccttcgcgtgatgtgaagctgacctttgcgcaaattgcaccgccgccgggcagcatggtgctacgtggcattaacccgaacggcagcattgagtttggtatgcgcagcgatgaagtggtgacgaaggcgatgctcaacctcgaatacaccccatcgccatcgttactgcctgtccagtcgcagttaaaggtttatctcaatgatgaactgatgggcgtgctgccagtgaccaaagaacagttgggtaaaaaaacgctggcgcaaatgcccattaacccactgtttattagcgacttcaaccgtgtacggctggagtttgtcggccattatcaggacgtgtgcgaaaaaccggccagcaccacgctttggctggatgttgggcggagcagtggactggatctgacctatcagaccctgaatgtgaagaatgacctgtcacacttcccggtgccattctttgacccgagcgataaccgcaccaacaccttgccgatggtctttgcgggtgcgccggatgttgggctgcaacaagcctctgccattgtcgcctcgtggtttggttcgcgttctggctggcgtgggcagaacttcccggtactctataaccaactgccggatcgcaatgccattgtctttgcaaccaacgacaaacggccggacttcctgcgcgatcatccggcggtaaaagccccggtgattgagatgattaaccatccgcagaatccttacgtcaaactgctggtggtgtttggtcgtgacgacaaagacctgttgcaggcagcgaaaggtatcgctcagggtaacattctgttccgtggtgaaagcgtggtagtgaatgaagtgaaaccgctgctaccgcgtaagccgtacgatgcgccgaactgggtacgtaccgatcgtccggtcacatttggcgaactgaaaacctatgaagaacagttacaatccagcggtcttgagccagcagcgattaacgtttcgctaaacctgccgccggatctctacctgatgcgcagtaccggcattgatatggatattaattaccgctacaccatgccgccggtgaaagacagttcgcggatggatatcagcctgaataaccagttcctgcaatccttcaacctgagcagcaaacaggaggcgaaccgcctgctgctgcggattccggtattacaaggtttgctggatggcaaaacagatgtctctattccggcgctgaaactgggcgcgaccaaccagctgcgcttcgactttgagtatatgaacccgatgccgggcggttcggtggataactgtattaccttccagccggtgcagaatcatgtggtgattggtgacgactccaccatcgacttctcgaagtattaccacttcatcccgatgccggatctacgcgcctttgctaacgcgggcttcccattcagccggatggcggatctgtcgcaaaccatcaccgtgatgccgaaagcgcctaacgaagcacagatggaaacgttgctgaatactgttggttttatcggcgcacagacgggcttcccggcgattaatctgacggtgaccgatgatggcagcaccattcagggcaaagatgccgacatcatgatcatcggtggtatcccggacaaactgaaagacgataagcagatcgacctattggtgcaggcgaccgaaagctgggtgaaaacaccgatgcgccagaccccgttccccggcattgtgccggacgagagcgatcgcgcggcagaaacccggtcaacgctgacctcttccggtgcgatggcggcggtgattggcttccagtcgccgtataacgaccagcgcagcgtgattgcgctgttggcagatagcccacgcggttatgaaatgcttaacgatgcggtgaacgatagcggcaaacgcgccaccatgttcggttcggtcgcggtgatccgcgagtccggtatcaacagcctacgtgttggcgacgtttattacgtaggtcatctgccgtggttcgagcgcgtgtggtatgcgctggcaaaccatccgattctgctggcggtgctggcggctatcagtgtgatattgctggcatgggtactgtggcgtctgctgcgaattattagtcgtcgtcgtcttaacccggataacgagtaa BBa_K861112_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattttactagagattaaagaggagaaatactagatgaaaagaaaactattctggatttgtgcagtggctatggggatgagtgcgttcccctctttcatgacgcaggcgacgccagcaacgcaaccactgatcaatgctgagccagctgtagccgcccagacggaacaaaatccgcaggtggggcaagtgatgccgggcgtgcagggcgctgatgcgccagtcgtggcgcagaacggtccttcgcgtgatgtgaagctgacctttgcgcaaattgcaccgccgccgggcagcatggtgctacgtggcattaacccgaacggcagcattgagtttggtatgcgcagcgatgaagtggtgacgaaggcgatgctcaacctcgaatacaccccatcgccatcgttactgcctgtccagtcgcagttaaaggtttatctcaatgatgaactgatgggcgtgctgccagtgaccaaagaacagttgggtaaaaaaacgctggcgcaaatgcccattaacccactgtttattagcgacttcaaccgtgtacggctggagtttgtcggccattatcaggacgtgtgcgaaaaaccggccagcaccacgctttggctggatgttgggcggagcagtggactggatctgacctatcagaccctgaatgtgaagaatgacctgtcacacttcccggtgccattctttgacccgagcgataaccgcaccaacaccttgccgatggtctttgcgggtgcgccggatgttgggctgcaacaagcctctgccattgtcgcctcgtggtttggttcgcgttctggctggcgtgggcagaacttcccggtactctataaccaactgccggatcgcaatgccattgtctttgcaaccaacgacaaacggccggacttcctgcgcgatcatccggcggtaaaagccccggtgattgagatgattaaccatccgcagaatccttacgtcaaactgctggtggtgtttggtcgtgacgacaaagacctgttgcaggcagcgaaaggtatcgctcagggtaacattctgttccgtggtgaaagcgtggtagtgaatgaagtgaaaccgctgctaccgcgtaagccgtacgatgcgccgaactgggtacgtaccgatcgtccggtcacatttggcgaactgaaaacctatgaagaacagttacaatccagcggtcttgagccagcagcgattaacgtttcgctaaacctgccgccggatctctacctgatgcgcagtaccggcattgatatggatattaattaccgctacaccatgccgccggtgaaagacagttcgcggatggatatcagcctgaataaccagttcctgcaatccttcaacctgagcagcaaacaggaggcgaaccgcctgctgctgcggattccggtattacaaggtttgctggatggcaaaacagatgtctctattccggcgctgaaactgggcgcgaccaaccagctgcgcttcgactttgagtatatgaacccgatgccgggcggttcggtggataactgtattaccttccagccggtgcagaatcatgtggtgattggtgacgactccaccatcgacttctcgaagtattaccacttcatcccgatgccggatctacgcgcctttgctaacgcgggcttcccattcagccggatggcggatctgtcgcaaaccatcaccgtgatgccgaaagcgcctaacgaagcacagatggaaacgttgctgaatactgttggttttatcggcgcacagacgggcttcccggcgattaatctgacggtgaccgatgatggcagcaccattcagggcaaagatgccgacatcatgatcatcggtggtatcccggacaaactgaaagacgataagcagatcgacctattggtgcaggcgaccgaaagctgggtgaaaacaccgatgcgccagaccccgttccccggcattgtgccggacgagagcgatcgcgcggcagaaacccggtcaacgctgacctcttccggtgcgatggcggcggtgattggcttccagtcgccgtataacgaccagcgcagcgtgattgcgctgttggcagatagcccacgcggttatgaaatgcttaacgatgcggtgaacgatagcggcaaacgcgccaccatgttcggttcggtcgcggtgatccgcgagtccggtatcaacagcctacgtgttggcgacgtttattacgtaggtcatctgccgtggttcgagcgcgtgtggtatgcgctggcaaaccatccgattctgctggcggtgctggcggctatcagtgtgatattgctggcatgggtactgtggcgtctgctgcgaattattagtcgtcgtcgtcttaacccggataacgagtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z