LOCUS BBa_K861124 2632 bp DNA linear 29-Nov-2024 DEFINITION lacI regulated FadE ACCESSION BBa_K861124 VERSION BBa_K861124.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 CDS 85..2529 /label=BBa_K861020 terminator 2538..2632 /label=BBa_B0024 misc_feature 64..2632 /label=BBa_K861022 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaaata ctagatgatg attttgagta ttctcgctac ggttgtcctg 121 ctcggcgcgt tgttctatca ccgcgtgagc ttatttatca gcagtctgat tttgctcgcc 181 tggacagcct ccctcggcgt tgctggtctg tggtcggcgt gggtactggt gcctctggcc 241 attatcctcg tgccatttaa ctttgcgcct atgcgtaagt cgatgatttc cgcgccggta 301 tttcgcggtt tccgtaaggt gatgccgccg atgtcgcgca ctgagaaaga agcgattgat 361 gcgggcacca cctggtggga gggcgacttg ttccagggca agccggactg gaaaaagctg 421 cataactatc cgcagccgcg cctgaccgcc gaagagcaag cgtttctcga cggcccggta 481 gaagaagcct gccggatggc gaatgatttc cagatcaccc atgagctggc ggatctgccg 541 ccggagttgt gggcgtacct taaagagcat cgtttcttcg cgatgatcat caaaaaagag 601 tacggcgggc tggagttctc ggcttatgcc cagtctcgcg tgctgcaaaa actctccggc 661 gtgagcggga tcctggcgat taccgtcggc gtgccaaact cattaggccc gggcgaactg 721 ttgcaacatt acggcactga cgagcagaaa gatcactatc tgccgcgtct ggcgcgtggt 781 caggagatcc cctgctttgc actgaccagc ccggaagcgg gttccgatgc gggcgcgatt 841 ccggacaccg ggattgtctg catgggcgaa tggcagggcc agcaggtgct ggggatgcgt 901 ctgacctgga acaaacgcta cattacgctg gcaccgattg cgactgtgct tgggctggcg 961 tttaaactct ccgacccgga aaagctgctg ggtggcgcag aagatttagg tattacctgt 1021 gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt cccgctgaac 1081 gtaccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat cgattacatc 1141 atcggtgggc cgaaaatggc cgggcaaggc tggcggatgc tggtggagtg cctctcggta 1201 ggccgcggca tcaccctgcc ttccaactca accggcggcg tgaaatcggt agcgctggca 1261 acgggcgcgt atgctcacat tcgccgtcag ttcaaaatct ctatcggtaa gatggaaggg 1321 attgaagagc cgctggcgcg tattgccggt aatgcctacg tgatggatgc tgcggcatcg 1381 ctgattacct acggcattat gctcggcgaa aaacctgccg tgctgtcggc tatcgttaag 1441 tatcactgta cccaccgcgg gcagcagtcg attattgatg cgatggatat cactggtggc 1501 aaaggcatta tgctcgggca gagcaacttc ctggcgcgtg cttaccaggg cgcaccgatt 1561 gccatcaccg ttgaaggggc caacattctg acccgcagca tgatgatctt cggacaagga 1621 gcgattcgtt gccatccgta cgtgctggaa gagatggaag cagcgaagaa caatgacgtc 1681 aacgcgttcg ataaactgtt gttcaaacat atcggtcacg tcggtagcaa caaagttcgc 1741 agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg cgatgccact 1801 aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc 1861 tcgatggctg tgctgggcgg cagcctgaaa cgtcgtgagc gcatctcggc acgtctgggg 1921 gatattttaa gccagcttta cctcgcctct gccgttctga agcgttatga cgacgaaggc 1981 cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac aagatgcgct gtatcaggct 2041 gaacaggcga tggatgattt actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg 2101 aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa gctggatcat 2161 aaagtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg tcgcggtcag 2221 tacctgacgc cgagcgagca taatccggtt ggcttgctgg aagaggcgct ggtggatgtg 2281 attgccgccg acccaattca tcagcggatc tgtaaagagc tgggtaaaaa cctgccgttt 2341 acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga taaagatgaa 2401 gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt tgatgacttt 2461 gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg gaaagttgaa 2521 gccgcgtaat actagagaaa taataaaaaa gccggattaa taatctggct ttttatattc 2581 tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagtgt ga //