BBa_K861171 1 Pcar Pcar, synthetic promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:36Z Escherichia coli str. K-12 substr. MG1655 This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183482 1 Consensus CRP binding site range2183482 1 15 36 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_K861130 1 BBa_K861130 BcsC,cellulose synthetase subunit 2012-09-06T11:00:00Z 2015-06-17T01:21:39Z Escherichia coli str. K-12 substr. MG1655 BcsA, BcsB, BcsZ and BcsC are involved in biosynthesis of cellulose, a characteristic of the rdar morphotype.Although bcsC is transcribed constitutively, cellulose synthesis occurs only when AdrA is express. false false _1121_ 0 4206 12357 9 Not in stock false None false Xian Xia BBa_K861132 1 BBa_K861132 BcsC controlled by promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsC is controlled by promoter PII which can be repressed by CRP-cAMP complex.So the expression of BcsC will be repressed at high concentration of CRP-cAMP complex.On the contrary,BcsC can be expressed at low concentration of CRP-cAMP complex. false false _1121_ 0 12357 9 Not in stock false None false Xian Xia component2182000 1 BBa_K861131 component2181994 1 BBa_K861171 annotation2181994 1 BBa_K861171 range2181994 1 1 36 annotation2182000 1 BBa_K861131 range2182000 1 45 3642 BBa_K861131 1 BBa_K861131 BcsC with RBS and terminator 2012-09-06T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 BcsA, BcsB, BcsZ and BcsC are involved in biosynthesis of cellulose, a characteristic of the rdar morphotype.Although bcsC is transcribed constitutively, cellulose synthesis occurs only when AdrA is express. false false _1121_ 0 12357 9 Not in stock false None false Xian Xia component2181956 1 BBa_K861130 component2181957 1 BBa_B0024 component2181954 1 BBa_B0030 annotation2181957 1 BBa_B0024 range2181957 1 3504 3598 annotation2181956 1 BBa_K861130 range2181956 1 22 3495 annotation2181954 1 BBa_B0030 range2181954 1 1 15 BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861131_sequence 1 attaaagaggagaaatactagatgcgcaaattcacactaaacatattcacgctttccctcggtctggccgtcatgccgatggtcgaggcagcaccaaccgctcagcaacagttgctggagcaagttcggttaggcgaagcgacccatcgtgaagatctggtgcaacagtcgttatatcggctggaacttattgatccgaataacccggacgtcgttgccgcccgtttccgttctttgttacgtcagggcgatattgatggcgcgcaaaaacagctcgatcggctgtcgcagttagcgccgagttcaaatgcgtataaatcgtcgcggactacgatgctactttccacgccggatggtcgtcaggcactgcaacaggcacgattgcaggcgacgaccggtcatgcagaagaagctgtggcgagttacaacaaactgttcaacggtgcgccgccggaaggtgacattgctgtcgagtactggagtacggtggcgaaaattccggctcgccgtggcgaagcgattaatcagttaaaacgcatcaatgcggatgcaccgggcaatacgggcctgcaaaacaatctggcgctattgctgtttagtagcgatcgccgtgacgaaggttttgccgtcctggaacagatggcaaaatcgaacgccgggcgcgaaggggcctctaaaatctggtacgggcagattaaagacatgcccgtcagtgatgccagtgtgtcggcgctgaaaaaatatctctcgatctttagtgatggcgatagcgtggcggctgcgcaatcgcaactggcagaacagcaaaaacagctggccgatcctgctttccgcgctcgtgcgcaaggtttagcggcggtggactctggtatggcgggtaaagccattcccgaactacaacaggcggtgcgggcgaacccgaaagacagtgaagctctgggggcgctgggccaggcgtattctcagaaaggcgatcgcgccaatgcagtggcgaatctggaaaaagccctcgcactggacccgcacagcagcaacaacgacaaatggaacagtctgctgaaagtaaaccgctactggctggcgatccagcagggcgatgctgcgctgaaagccaataatcctgaccgggcagaacgcctgttccagcaggcgcgtaatgtcgataacaccgacagttatgcagtgctggggctgggcgatgtggcgatggcgcgaaaagattatcccgccgccgaacgttattatcagcagaccttgcgtatggacagcggcaacactaacgccgtgcgcgggctggcaaatatttaccgccagcaatcgccagaaaaagctgaagcgtttatcgcctcgctctctgccagtcagcggcgtagcattgatgatatcgaacgcagcctgcaaaacgaccgtctggcacagcaggcagaggcactggaaaaccagggcaaatgggcgcaggcggcagcacttcagcggcaacgactggcgctggaccccggcagcgtatggattacttaccgactttcgcaggatctctggcaggccggacaacgcagccaggccgatacgttaatgcgcaatctggcgcagcagaagtcgaacgacccggagcaggtttacgcttacgggctgtacctctctggtcatgaccaggacagagcggcgctggcgcatatcaatagcctgccgcgtgcgcagtggaacagcaatattcaggagctggttaatcgactgcaaagcgatcaggtgctggaaaccgctaaccgcctgcgagaaagcggcaaagaggcagaagcggaagcgatgctgcgccagcaaccaccttccacgcgtattgacctcacgctggctgactgggcgcaacaacgacgtgattacaccgccgcccgcgctgcatatcagaatgtcctgacgcgggagccagctaacgccgacgccattcttggtctgacggaagtggatattgctgccggtgacaaagcggcggcacgtagccagctggcgaaactgcccgctaccgataacgcctcgctgaacacacagcggcgcgtggcgctggcacaggcgcagcttggcgataccgcagcagcgcagcggacgtttaataagttgatcccgcaggcaaaatctcagccaccgtcgatggaaagcgcgatggtgctgcgtgatggtgcgaagtttgaagcgcaggcgggcgatccaacgcaggcgctggaaacctacaaagacgccatggtcgcatccggtgtgactacgacgcgtccgcaggataacgacacctttacccgactgacccgtaacgacgagaaagatgactggctgaaacgtggcgtgcgcagcgatgcggcggacctctatcgccagcaggatcttaacgtcacccttgagcacgattactggggttcgagcggcaccggtggttactccgatctgaaagcgcacactaccatgttgcaggtggatgcgccgtattctgacgggcggatgttctttcgcagtgatttcgtcaatatgaacgtcggcagtttctccactaatgccgatggcaaatgggatgacaactggggcacctgtacattacaggactgtagcggcaaccgcagccagtcggattccggtgccagcgtggcggtcggctggcgaaatgacgtctggagctgggatatcggtaccacgccgatgggcttcaacgtggtggatgtggtcggcggcatcagttacagcgatgatatcgggccgctgggttacaccgttaacgcccaccgtcggcccatctccagttctttgctggcctttggtgggcaaaaagactccccgagcaataccgggaaaaaatggggtggcgtacgtgccgacggtgtggggctaagtctgagctacgataaaggtgaagcaaacggcgtctgggcatcgcttagtggcgaccagttaaccggtaaaaatgtcgaagataactggcgcgtgcgctggatgacgggctattactataaggtcattaaccagaacaatcgccgcgtcacaatcggcctgaacaacatgatctggcattacgacaaagatctgagtggctactcactcggtcagggcggttactacagtccgcaggaatacctgtcgtttgccataccggtgatgtggcgggagcgcacggaaaactggtcgtgggagctgggtgcgtctggctcgtggtcgcattcacgcaccaaaaccatgccgcgttatccgctgatgaatctgatcccgaccgactggcaggaagaagctgcgcggcaatccaacgatggcggcagcagtcagggcttcggctacacggcgcgggcattacttgaacgacgtgttacttccaactggtttgttggcacggcaattgatatccagcaggcgaaagattacgcacccagccatttcctgctctacgtacgttattccgccgccggatggcagggtgacatggatttaccgccgcagccgctgataccttacgccgactggtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861171_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattt BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861130_sequence 1 atgcgcaaattcacactaaacatattcacgctttccctcggtctggccgtcatgccgatggtcgaggcagcaccaaccgctcagcaacagttgctggagcaagttcggttaggcgaagcgacccatcgtgaagatctggtgcaacagtcgttatatcggctggaacttattgatccgaataacccggacgtcgttgccgcccgtttccgttctttgttacgtcagggcgatattgatggcgcgcaaaaacagctcgatcggctgtcgcagttagcgccgagttcaaatgcgtataaatcgtcgcggactacgatgctactttccacgccggatggtcgtcaggcactgcaacaggcacgattgcaggcgacgaccggtcatgcagaagaagctgtggcgagttacaacaaactgttcaacggtgcgccgccggaaggtgacattgctgtcgagtactggagtacggtggcgaaaattccggctcgccgtggcgaagcgattaatcagttaaaacgcatcaatgcggatgcaccgggcaatacgggcctgcaaaacaatctggcgctattgctgtttagtagcgatcgccgtgacgaaggttttgccgtcctggaacagatggcaaaatcgaacgccgggcgcgaaggggcctctaaaatctggtacgggcagattaaagacatgcccgtcagtgatgccagtgtgtcggcgctgaaaaaatatctctcgatctttagtgatggcgatagcgtggcggctgcgcaatcgcaactggcagaacagcaaaaacagctggccgatcctgctttccgcgctcgtgcgcaaggtttagcggcggtggactctggtatggcgggtaaagccattcccgaactacaacaggcggtgcgggcgaacccgaaagacagtgaagctctgggggcgctgggccaggcgtattctcagaaaggcgatcgcgccaatgcagtggcgaatctggaaaaagccctcgcactggacccgcacagcagcaacaacgacaaatggaacagtctgctgaaagtaaaccgctactggctggcgatccagcagggcgatgctgcgctgaaagccaataatcctgaccgggcagaacgcctgttccagcaggcgcgtaatgtcgataacaccgacagttatgcagtgctggggctgggcgatgtggcgatggcgcgaaaagattatcccgccgccgaacgttattatcagcagaccttgcgtatggacagcggcaacactaacgccgtgcgcgggctggcaaatatttaccgccagcaatcgccagaaaaagctgaagcgtttatcgcctcgctctctgccagtcagcggcgtagcattgatgatatcgaacgcagcctgcaaaacgaccgtctggcacagcaggcagaggcactggaaaaccagggcaaatgggcgcaggcggcagcacttcagcggcaacgactggcgctggaccccggcagcgtatggattacttaccgactttcgcaggatctctggcaggccggacaacgcagccaggccgatacgttaatgcgcaatctggcgcagcagaagtcgaacgacccggagcaggtttacgcttacgggctgtacctctctggtcatgaccaggacagagcggcgctggcgcatatcaatagcctgccgcgtgcgcagtggaacagcaatattcaggagctggttaatcgactgcaaagcgatcaggtgctggaaaccgctaaccgcctgcgagaaagcggcaaagaggcagaagcggaagcgatgctgcgccagcaaccaccttccacgcgtattgacctcacgctggctgactgggcgcaacaacgacgtgattacaccgccgcccgcgctgcatatcagaatgtcctgacgcgggagccagctaacgccgacgccattcttggtctgacggaagtggatattgctgccggtgacaaagcggcggcacgtagccagctggcgaaactgcccgctaccgataacgcctcgctgaacacacagcggcgcgtggcgctggcacaggcgcagcttggcgataccgcagcagcgcagcggacgtttaataagttgatcccgcaggcaaaatctcagccaccgtcgatggaaagcgcgatggtgctgcgtgatggtgcgaagtttgaagcgcaggcgggcgatccaacgcaggcgctggaaacctacaaagacgccatggtcgcatccggtgtgactacgacgcgtccgcaggataacgacacctttacccgactgacccgtaacgacgagaaagatgactggctgaaacgtggcgtgcgcagcgatgcggcggacctctatcgccagcaggatcttaacgtcacccttgagcacgattactggggttcgagcggcaccggtggttactccgatctgaaagcgcacactaccatgttgcaggtggatgcgccgtattctgacgggcggatgttctttcgcagtgatttcgtcaatatgaacgtcggcagtttctccactaatgccgatggcaaatgggatgacaactggggcacctgtacattacaggactgtagcggcaaccgcagccagtcggattccggtgccagcgtggcggtcggctggcgaaatgacgtctggagctgggatatcggtaccacgccgatgggcttcaacgtggtggatgtggtcggcggcatcagttacagcgatgatatcgggccgctgggttacaccgttaacgcccaccgtcggcccatctccagttctttgctggcctttggtgggcaaaaagactccccgagcaataccgggaaaaaatggggtggcgtacgtgccgacggtgtggggctaagtctgagctacgataaaggtgaagcaaacggcgtctgggcatcgcttagtggcgaccagttaaccggtaaaaatgtcgaagataactggcgcgtgcgctggatgacgggctattactataaggtcattaaccagaacaatcgccgcgtcacaatcggcctgaacaacatgatctggcattacgacaaagatctgagtggctactcactcggtcagggcggttactacagtccgcaggaatacctgtcgtttgccataccggtgatgtggcgggagcgcacggaaaactggtcgtgggagctgggtgcgtctggctcgtggtcgcattcacgcaccaaaaccatgccgcgttatccgctgatgaatctgatcccgaccgactggcaggaagaagctgcgcggcaatccaacgatggcggcagcagtcagggcttcggctacacggcgcgggcattacttgaacgacgtgttacttccaactggtttgttggcacggcaattgatatccagcaggcgaaagattacgcacccagccatttcctgctctacgtacgttattccgccgccggatggcagggtgacatggatttaccgccgcagccgctgataccttacgccgactggtaa BBa_K861132_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattttactagagattaaagaggagaaatactagatgcgcaaattcacactaaacatattcacgctttccctcggtctggccgtcatgccgatggtcgaggcagcaccaaccgctcagcaacagttgctggagcaagttcggttaggcgaagcgacccatcgtgaagatctggtgcaacagtcgttatatcggctggaacttattgatccgaataacccggacgtcgttgccgcccgtttccgttctttgttacgtcagggcgatattgatggcgcgcaaaaacagctcgatcggctgtcgcagttagcgccgagttcaaatgcgtataaatcgtcgcggactacgatgctactttccacgccggatggtcgtcaggcactgcaacaggcacgattgcaggcgacgaccggtcatgcagaagaagctgtggcgagttacaacaaactgttcaacggtgcgccgccggaaggtgacattgctgtcgagtactggagtacggtggcgaaaattccggctcgccgtggcgaagcgattaatcagttaaaacgcatcaatgcggatgcaccgggcaatacgggcctgcaaaacaatctggcgctattgctgtttagtagcgatcgccgtgacgaaggttttgccgtcctggaacagatggcaaaatcgaacgccgggcgcgaaggggcctctaaaatctggtacgggcagattaaagacatgcccgtcagtgatgccagtgtgtcggcgctgaaaaaatatctctcgatctttagtgatggcgatagcgtggcggctgcgcaatcgcaactggcagaacagcaaaaacagctggccgatcctgctttccgcgctcgtgcgcaaggtttagcggcggtggactctggtatggcgggtaaagccattcccgaactacaacaggcggtgcgggcgaacccgaaagacagtgaagctctgggggcgctgggccaggcgtattctcagaaaggcgatcgcgccaatgcagtggcgaatctggaaaaagccctcgcactggacccgcacagcagcaacaacgacaaatggaacagtctgctgaaagtaaaccgctactggctggcgatccagcagggcgatgctgcgctgaaagccaataatcctgaccgggcagaacgcctgttccagcaggcgcgtaatgtcgataacaccgacagttatgcagtgctggggctgggcgatgtggcgatggcgcgaaaagattatcccgccgccgaacgttattatcagcagaccttgcgtatggacagcggcaacactaacgccgtgcgcgggctggcaaatatttaccgccagcaatcgccagaaaaagctgaagcgtttatcgcctcgctctctgccagtcagcggcgtagcattgatgatatcgaacgcagcctgcaaaacgaccgtctggcacagcaggcagaggcactggaaaaccagggcaaatgggcgcaggcggcagcacttcagcggcaacgactggcgctggaccccggcagcgtatggattacttaccgactttcgcaggatctctggcaggccggacaacgcagccaggccgatacgttaatgcgcaatctggcgcagcagaagtcgaacgacccggagcaggtttacgcttacgggctgtacctctctggtcatgaccaggacagagcggcgctggcgcatatcaatagcctgccgcgtgcgcagtggaacagcaatattcaggagctggttaatcgactgcaaagcgatcaggtgctggaaaccgctaaccgcctgcgagaaagcggcaaagaggcagaagcggaagcgatgctgcgccagcaaccaccttccacgcgtattgacctcacgctggctgactgggcgcaacaacgacgtgattacaccgccgcccgcgctgcatatcagaatgtcctgacgcgggagccagctaacgccgacgccattcttggtctgacggaagtggatattgctgccggtgacaaagcggcggcacgtagccagctggcgaaactgcccgctaccgataacgcctcgctgaacacacagcggcgcgtggcgctggcacaggcgcagcttggcgataccgcagcagcgcagcggacgtttaataagttgatcccgcaggcaaaatctcagccaccgtcgatggaaagcgcgatggtgctgcgtgatggtgcgaagtttgaagcgcaggcgggcgatccaacgcaggcgctggaaacctacaaagacgccatggtcgcatccggtgtgactacgacgcgtccgcaggataacgacacctttacccgactgacccgtaacgacgagaaagatgactggctgaaacgtggcgtgcgcagcgatgcggcggacctctatcgccagcaggatcttaacgtcacccttgagcacgattactggggttcgagcggcaccggtggttactccgatctgaaagcgcacactaccatgttgcaggtggatgcgccgtattctgacgggcggatgttctttcgcagtgatttcgtcaatatgaacgtcggcagtttctccactaatgccgatggcaaatgggatgacaactggggcacctgtacattacaggactgtagcggcaaccgcagccagtcggattccggtgccagcgtggcggtcggctggcgaaatgacgtctggagctgggatatcggtaccacgccgatgggcttcaacgtggtggatgtggtcggcggcatcagttacagcgatgatatcgggccgctgggttacaccgttaacgcccaccgtcggcccatctccagttctttgctggcctttggtgggcaaaaagactccccgagcaataccgggaaaaaatggggtggcgtacgtgccgacggtgtggggctaagtctgagctacgataaaggtgaagcaaacggcgtctgggcatcgcttagtggcgaccagttaaccggtaaaaatgtcgaagataactggcgcgtgcgctggatgacgggctattactataaggtcattaaccagaacaatcgccgcgtcacaatcggcctgaacaacatgatctggcattacgacaaagatctgagtggctactcactcggtcagggcggttactacagtccgcaggaatacctgtcgtttgccataccggtgatgtggcgggagcgcacggaaaactggtcgtgggagctgggtgcgtctggctcgtggtcgcattcacgcaccaaaaccatgccgcgttatccgctgatgaatctgatcccgaccgactggcaggaagaagctgcgcggcaatccaacgatggcggcagcagtcagggcttcggctacacggcgcgggcattacttgaacgacgtgttacttccaactggtttgttggcacggcaattgatatccagcaggcgaaagattacgcacccagccatttcctgctctacgtacgttattccgccgccggatggcagggtgacatggatttaccgccgcagccgctgataccttacgccgactggtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z