LOCUS       BBa_K861173             1000 bp    DNA     linear       08-May-2024
DEFINITION  mRFP generator controlled by  PcstA (glucose-repressible promoter)
ACCESSION   BBa_K861173
VERSION     BBa_K861173.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     CDS             158..832
                     /label=mrfp1
     misc_feature    839..863
                     /label=Help:Barcodes
     CDS             158..838
                     /label=BBa_E1010
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
     misc_feature    140..1000
                     /label=BBa_I13507
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
      181 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      241 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      301 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      361 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      421 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      481 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      541 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      601 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
      661 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
      721 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
      781 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      841 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//